Addgene

We narrowed to 470 results for: Chuk

Showing: 241 - 280 of 470 results

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pDON207 NSP3 K977Q

Plasmid

#180057

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 NSP3 K977Q (ORF1ab SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

NSP3 K977Q

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON207 NSP3 A890D

Plasmid

#180069

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 NSP3 A890D (ORF1ab SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

NSP3 A890D

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON221 ORF3a Q57H

Plasmid

#180072

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 ORF3a Q57H (ORF3a SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

ORF3a Q57H

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 N S235F

Plasmid

#180071

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 N S235F (N SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

N S235F

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 NSP5 K90R

Plasmid

#180073

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 NSP5 K90R (ORF1ab SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

NSP5 K90R

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-TMPRSS11B

Plasmid

#172421

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Transmembrane protease serine 11B (TMPRSS11B Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA4/TO-ORF24 1-201-2xStrep

Plasmid

#138442

Purpose

Expresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-201

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-ORF24 1-226-2xStrep

Plasmid

#138443

Purpose

Expresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-226

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-BcRF1 1-168-2xStrep

Plasmid

#138447

Purpose

Expresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1 (BcRF1 EBV (HHV-4))

Use

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-168

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-BcRF1 1-178-2xStrep

Plasmid

#138448

Purpose

Expresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1 (BcRF1 EBV (HHV-4))

Use

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-178

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-BcRF1 1-203-2xStrep

Plasmid

#138449

Purpose

Expresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1 (BcRF1 EBV (HHV-4))

Use

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-203

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-UL87 1-238-2xStrep

Plasmid

#138450

Purpose

Expresses truncated UL87 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

UL87 (UL87 HCMV (HHV-5))

Use

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-238

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-UL87 1-248-2xStrep

Plasmid

#138451

Purpose

Expresses truncated UL87 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

UL87 (UL87 HCMV (HHV-5))

Use

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-248

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-UL87 1-273-2xStrep

Plasmid

#138452

Purpose

Expresses truncated UL87 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

UL87 (UL87 HCMV (HHV-5))

Use

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-273

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-mu24 3L_A-2xStrep

Plasmid

#138438

Purpose

Expresses full-length mu24 3L_A with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

mu24

Use

Tags

Strep

Expression

Mammalian

Mutation

Amino acids L64-L66 mutated to alanine

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-ORF24 1-191-2xStrep

Plasmid

#138441

Purpose

Expresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-191

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-ORF24 1-201-3xFLAG

Plasmid

#138420

Purpose

Expresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 1-201 (ORF24 KSHV (HHV-8))

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids 1-201

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-ORF24 1-271-3xFLAG

Plasmid

#138421

Purpose

Expresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 1-271 (ORF24 KSHV (HHV-8))

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids 1-271

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-ORF24 1-133-3xFLAG

Plasmid

#138422

Purpose

Expresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 1-133 (ORF24 KSHV (HHV-8))

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids 1-133

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDK616

Plasmid

#134202

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD) mutant

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

K223A K226A

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pIW040

Plasmid

#134127

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

aa 132-255 R236A

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pIW039

Plasmid

#134126

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

aa 132-255 and K203A K206A

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIW028

Plasmid

#134125

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

aa 132-255 and K183A K184A

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIW070

Plasmid

#134138

Purpose

Retroviral expression of GFP-Ska1

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

GFP-human Ska1 delta MTBD CRISPR/Cas9 hardened (SKA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

residues 1-131, CRISPR resistant

Promoter

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC-TFRC

Plasmid

#172420

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Transferrin receptor protein 1 (TfR) (CD antigen CD71) (TFRC Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLV-Bsd-CMV-hUCK

Plasmid

#195141

Purpose

UCK2 overexpression vector with blasticidin resistance

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

UCK2 (UCK2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAnc80L65

Plasmid

#68837

Purpose

Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details

Depositor

Luk Vandenberghe

Article

Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

Insert

Ancestral AAV Capsid Anc80L65

Use

AAV and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Rep2

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Chr1q_Centromere-Targeting_gRNA

Plasmid

#195125

Purpose

gRNA targeting centromere-proximal location on Chromosome 1q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Chr7_Centromere-Targeting_gRNA

Plasmid

#195129

Purpose

dual gRNA vector targeting centromere-proximal locations on Chromosome 7p and 7q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr7 gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1_gRNA

Plasmid

#196139

Purpose

gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

AAVS1 gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQLink-GST-10xCTD

Plasmid

#138472

Purpose

Expresses GST-tagged 10xCTD heptapeptide repeat in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

Rpb1 (POLR2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

10x CTD repeat

Promoter

T5

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Chr8q_Centromere-Targeting_gRNA

Plasmid

#195128

Purpose

gRNA targeting centromere-proximal location on Chromosome 8q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr8q gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Rosa26_gRNA

Plasmid

#196138

Purpose

gRNA targeting the Rosa26 locus in a thid generation Cas9 backbone with TagBFP2

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Rosa26 gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR6K6_Deletion_gRNA2

Plasmid

#195196

Purpose

dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

OR6K6 dual gRNA (OR6K6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR6K6_Deletion_gRNA1

Plasmid

#195195

Purpose

dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

OR6K6 dual gRNA (OR6K6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR6K6_Deletion_gRNA3

Plasmid

#195197

Purpose

dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

OR6K6 dual gRNA (OR6K6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC-CTSL

Plasmid

#172414

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Procathepsin L (Cathepsin L1) (CTSL Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MAC-TMPRSS4

Plasmid

#172422

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Transmembrane protease serine 4 (TMPRSS4 Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mito-yHS1-M7A

Plasmid

#159164

Purpose

Mitochondrial labile heme reporter

Depositor

Amit Reddi

Article

Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

Insert

mito-yHS1-M7A

Use

Tags

COX4 pre-sequence

Expression

Yeast

Mutation

Mutated Met 7 of the cyt b562 module of mito-yHS1…

Promoter

GPD

Available since

Sept. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGEX4T1-Rpb4/7

Plasmid

#138484

Purpose

Expresses GST-tagged Rpb4 and His-tagged Rpb7 in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Inserts

Rpb4 (POLR2D Human)
Rpb7 (POLR2G Human)

Use

Tags

6xHis and GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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