We narrowed to 16,751 results for: EMB

1. pccGFP-MalE-pos-1F02

   Plasmid

   #239087

   Purpose

   Positive control for liquid MalE complementation assay

   Depositor

   Alessandro Senes

   Article

   Anderson et al bioRxiv 2025.04.22.650048

   Insert

   transmembrane domain of AMPE
   

   Use

   Tags

   MBP and ToxR

   Expression

   Bacterial

   Mutation

   deleted amino acids 19-22, and changed Leucine 35…

   Promoter

   Available since

   June 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pccGFP-MalE-pos-2H07

   Plasmid

   #239088

   Purpose

   Positive control for liquid MalE complementation assay

   Depositor

   Alessandro Senes

   Article

   Anderson et al bioRxiv 2025.04.22.650048

   Insert

   transmembrane domain of FND10
   

   Use

   Tags

   MBP and ToxR

   Expression

   Bacterial

   Mutation

   Changed Valine 184 to Isoleucine

   Promoter

   Available since

   June 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pccGFP-MalE-neg-2E11

   Plasmid

   #239084

   Purpose

   Negative control for liquid MalE complementation assay

   Depositor

   Alessandro Senes

   Article

   Anderson et al bioRxiv 2025.04.22.650048

   Insert

   transmembrane domain of LRIG3
   

   Use

   Tags

   MBP and ToxR

   Expression

   Bacterial

   Mutation

   Changed Valine 827 to Isoleucine

   Promoter

   Available since

   June 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pccGFP-MalE-neg-2F12

   Plasmid

   #239085

   Purpose

   Negative control for liquid MalE complementation assay

   Depositor

   Alessandro Senes

   Article

   Anderson et al bioRxiv 2025.04.22.650048

   Insert

   transmembrane domain of LRIG3
   

   Use

   Tags

   MBP and ToxR

   Expression

   Bacterial

   Mutation

   Changed Valine 815 to Isoleucine

   Promoter

   Available since

   June 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PB-Blast-Dest-LifeAct-mRuby

   Plasmid

   #194907

   Purpose

   To generate LifeAct-mRuby expressing stable cell lines with piggybac transposase co-expression and blastocidin selections.

   Depositor

   Clare Buckley

   Article

   Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

   Insert

   LifeAct
   

   Use

   Mouse Targeting

   Tags

   mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGL4.26_4xUAS_DSCP_GFP

   Plasmid

   #111931

   Purpose

   Vector to express GFP (under the control of the DSCP and an array of 4 UAS sites)

   Depositor

   Alexander Stark

   Article

   Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

   Insert

   4xUAS GFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. C1-MPAct-mCherry

   Plasmid

   #155222

   Purpose

   Monitor changes in the density of membrane-proximal F-actin (MPA)

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. C1-MPAct-mCitrine

   Plasmid

   #155220

   Purpose

   Monitor changes in the density of membrane-proximal F-actin (MPA)

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. C1-MPAct-mRuby3

   Plasmid

   #155221

   Purpose

   Monitor changes in the density of membrane-proximal F-actin (MPA)

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
   

   Use

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-cMSP1D1

    Plasmid

    #199768

    Purpose

    Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSP1D1
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    external His-Tags flanking the C and N termini

    Promoter

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-cMSPdH5

    Plasmid

    #199771

    Purpose

    Expresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH5
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    external His-Tags flanking the C and N termini

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p-mCherry2-sgRNA (empty)

    Plasmid

    #198330

    Purpose

    Customisable sgRNA sequence with optimised sgRNA scaffold for expression under U6 promoter, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    U6-sgRNA(F+E) empty
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-cMSPdH45

    Plasmid

    #199767

    Purpose

    Expresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH45
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    internal His-Tag

    Promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO.1 Blast NT shRNA

    Plasmid

    #123306

    Purpose

    Expression of a non-targeting shRNA

    Depositor

    Giovanni Tonon

    Article

    Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

    Insert

    Non-targeting shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TRAPa-3xFLAG-pCDNA

    Plasmid

    #201963

    Purpose

    Mammalian expression of Translocon-associated protein subunit alpha

    Depositor

    Ville Paavilainen

    Article

    Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

    Insert

    SSR1 (SSR1 Human)
    

    Use

    Tags

    3xFLAG-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-cMSP26

    Plasmid

    #199769

    Purpose

    Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSP26
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    internal His-Tag

    Promoter

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-cMSPdH4-6

    Plasmid

    #199766

    Purpose

    Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH46
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    internal His-Tag

    Promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pML2(−)WF

    Plasmid

    #108337

    Purpose

    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pML2(+)BR

    Plasmid

    #108336

    Purpose

    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pML2(+)WF

    Plasmid

    #108333

    Purpose

    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pML2(+)BF

    Plasmid

    #108334

    Purpose

    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pML2(+)WR

    Plasmid

    #108335

    Purpose

    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pML2(−)BF

    Plasmid

    #108338

    Purpose

    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pML2(−)WR

    Plasmid

    #108339

    Purpose

    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pML2(−)BR

    Plasmid

    #108340

    Purpose

    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEF-DEST51 LacZ

    Plasmid

    #123292

    Purpose

    Mammalian expression of control truncated LacZ

    Depositor

    Giovanni Tonon

    Article

    Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

    Insert

    LacZ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pccGFP-MalE-neg-1E01

    Plasmid

    #239013

    Purpose

    Negative control for liquid MalE complementation assay

    Depositor

    Alessandro Senes

    Article

    Anderson et al bioRxiv 2025.04.22.650048

    Insert

    transmembrane domain of GBGT1
    

    Use

    Tags

    MBP and ToxR

    Expression

    Bacterial

    Mutation

    Changed Leucine 6 to phenylalanine, changed Leuci…

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pccGFP-MalE-neg-1E11

    Plasmid

    #239083

    Purpose

    Negative control for liquid MalE complementation assay

    Depositor

    Alessandro Senes

    Article

    Anderson et al bioRxiv 2025.04.22.650048

    Insert

    Transmembrane domain of CEA20
    

    Use

    Tags

    MBP and ToxR

    Expression

    Bacterial

    Mutation

    Changed Alanine 457 to Phenylalanine, and deleted…

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TRAPa(R150A)-3xFLAG-pCDNA

    Plasmid

    #201966

    Purpose

    Mammalian expression of mutated Translocon-associated protein subunit alpha

    Depositor

    Ville Paavilainen

    Article

    Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

    Insert

    SSR1 (SSR1 Human)
    

    Use

    Tags

    3xFLAG-tag

    Expression

    Mammalian

    Mutation

    R150A

    Promoter

    Available since

    Jan. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP VAMP3

    Plasmid

    #42310

    Depositor

    Thierry Galli

    Article

    Galli et al Mol Biol Cell. 1998 Jun;9(6):1437-48.

    Insert

    VAMP 3 (VAMP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. EGFP-hPMCA4b

    Plasmid

    #47589

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA4x/b (ATP2B4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K442R and P673L relative to NP_001675.3

    Promoter

    CMV

    Available since

    Aug. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLenti c-MYC-DDK-Puro GFP

    Plasmid

    #123299

    Purpose

    Mammalian expression of GFP C-MYC-FLAG

    Depositor

    Giovanni Tonon

    Article

    Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    MYC-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 14x PP7 with loxP-kanMX-loxP

    Plasmid

    #189939

    Purpose

    Integration of 14xPP7 loops in Saccharomyces cerevisiae. Selection by KanMX (flanked by LoxP sites for marker removal)

    Depositor

    Tineke Lenstra

    Article

    Donovan et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

    Insert

    14x PP7 with loxP-kanMX-loxP
    

    Use

    Tags

    kanMX

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMM2-hPMCA2w/b

    Plasmid

    #47583

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2w/b (ATP2B2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMM2-hPMCA2z/b

    Plasmid

    #47581

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2z/b (ATP2B2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEF-DEST51 HA-PNRC1

    Plasmid

    #123294

    Purpose

    Mammalian expression of PNRC1 N-HA

    Depositor

    Giovanni Tonon

    Article

    Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

    Insert

    PNRC1 (PNRC1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p-mCherry2-sgC9cen

    Plasmid

    #198326

    Purpose

    Expresses a guide RNA against a repetitive locus found in the pericentromere of human chromosome 9, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    Chr9-CEN guide RNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMM2-hPMCA2x/b

    Plasmid

    #47582

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2x/b (ATP2B2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFR092

    Plasmid

    #134148

    Purpose

    Expression of CENP-T

    Depositor

    Iain Cheeseman

    Article

    Nishino et al EMBO J. 2013 Feb 6;32(3):424-36. doi: 10

    Insert

    CENP-T (CENPT Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 1-375

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. EGFP-hPMCA4b delta6

    Plasmid

    #47590

    Depositor

    Emanuel Strehler

    Article

    Padanyi et al Biochim Biophys Acta. 2009 Jun;1793(6):1

    Insert

    PMCA4bdelta6 (ATP2B4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    6 C-terminal AA deleted (needed for PDZ binding)

    Promoter

    CMV

    Available since

    Aug. 26, 2013

    Availability

    Academic Institutions and Nonprofits only