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We narrowed to 1,499 results for: U6 promoter

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Showing: 241 - 280 of 1499 results

  1. KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid
    #121814
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121814-plasmid-map-sequence-id-237361

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  2. KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter

    Plasmid
    #121813
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    March 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    121813-plasmid-map-sequence-id-239026

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  3. KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid
    #121819
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121819-plasmid-map-sequence-id-237368

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  4. KB701: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; CMV promoter

    Plasmid
    #121818
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121818-plasmid-map-sequence-id-237367

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  6. pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori

    Plasmid
    #234724
    Purpose
    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.
    Depositor
    Kirill Martemyanov
    Article
    Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    T2A-GFP
    Expression
    Mammalian
    Promoter
    CAG promoter
    Available Since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    234724-plasmid-map-sequence-id-466565

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  16. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82706
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82706-plasmid-map-sequence-id-158046

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  17. pJEP11-AAV-U6/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82705
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    82705-plasmid-map-sequence-id-170450

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  22. pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159501
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-2_PolyU(1)_HairpinDistance(10)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159501-plasmid-map-sequence-id-311777

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  23. pAV-U6+27-Linear-2_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159502
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-2_PolyU(4)_HairpinDistance(10)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159502-plasmid-map-sequence-id-311727

    Enlarge

  24. pAV-U6+27-Linear-3_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159504
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(1)_HairpinDistance(10)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159504-plasmid-map-sequence-id-311728

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  25. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159506
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(10)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159506-plasmid-map-sequence-id-311732

    Enlarge

  26. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid
    #159508
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(0)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159508-plasmid-map-sequence-id-311756

    Enlarge

  27. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(16)nts_Tornado-Corn

    Plasmid
    #159511
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 16 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(16)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159511-plasmid-map-sequence-id-311774

    Enlarge

  28. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(19)nts_Tornado-Corn

    Plasmid
    #159514
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 19 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(19)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159514-plasmid-map-sequence-id-311759

    Enlarge

  29. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(20)nts_Tornado-Corn

    Plasmid
    #159515
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 20 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(20)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159515-plasmid-map-sequence-id-311758

    Enlarge

  30. pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid
    #159499
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-2_PolyU(1)_HairpinDistance(0)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159499-plasmid-map-sequence-id-311745

    Enlarge

  31. pAV-U6+27-Linear-1_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159498
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-1_PolyU(4)_HairpinDistance(10)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159498-plasmid-map-sequence-id-311780

    Enlarge

  32. pAV-U6+27-Linear-1_PolyU(4) _HairpinDistance(0)_Tornado-Corn

    Plasmid
    #159496
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-1_PolyU(4) _HairpinDistance(0)
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159496-plasmid-map-sequence-id-311762

    Enlarge

  33. pAV-U6+27-Linear-1_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid
    #159495
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-1_PolyU(1)_HairpinDistance(0)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159495-plasmid-map-sequence-id-311767

    Enlarge

  34. pDGB3 alpha1 U6-26:gRNA 4 (Pnos):2.1 aptamer (GB1724)

    Plasmid
    #160621
    Purpose
    Target for the Nopaline Synthetase Promoter with the MS2 recognition 2.1 loop in position3' in the scaffold
    Depositor
    Diego Orzaez
    Article
    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-
    Insert
    U6-26:gRNA 4 (pNos): 2.1 aptamer
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    U6-26
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160621-plasmid-map-sequence-id-322006

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  36. PB-2G-CNCB_sgRBBP6-68

    Plasmid
    #237555
    Purpose
    A piggybac-based vector containing mouse U6 promoter-driven RBBP6 sgRNA #6, human U6 promoter-driven RBBP6 sgRNA #8 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.
    Depositor
    Kazutoshi Takahashi
    Article
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Insert
    RBBP6 (RBBP6 Human)
    Use
    Piggybac
    Expression
    Mammalian
    Promoter
    mouse U6 promoter and mouse U6 promoter, human U6…
    Available Since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    237555-plasmid-map-sequence-id-473543

    Enlarge

  37. pEJS-HZ79 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26_V2

    Plasmid
    #199261
    Purpose
    Optimized single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene. This optimized construct showed improved in vivo editing efficiency.
    Depositor
    Erik Sontheimer
    Article
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    U1a promoter
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199261-plasmid-map-sequence-id-389819

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  39. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #191797
    Purpose
    vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized dCas13d
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    R239A, H244A, R858A, H863A
    Promoter
    CBh, SV40, hU6
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191797-plasmid-map-sequence-id-375579

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Showing: 241 - 280 of 1499 results