We narrowed to 1,651 results for: Tef
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Plasmid#166449PurposeExpresses fusion of CRY2PHR with mCherry and TEAD4-IDRDepositorAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only
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PHBA02
Plasmid#83536PurposeConstitutive TEF1 promoter mediated expression of the UBiC and ARO4* genesDepositorInsertpTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t
ExpressionYeastPromoterpTEF1Available SinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJT1
Plasmid#98299PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
ExpressionYeastAvailable SinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJT2
Plasmid#98306PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
ExpressionYeastMutationERG20 N127WAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRHD-TetR-GAL80-Rho1-GAL4
Plasmid#218274PurposeIntroduction of low-temperature-inducible GAL system, LowTempGAL v2.2DepositorInsertgal80Arm1-pAgTEF1>KanMX4>tAgTEF1-pHAC1>UBI4>>DHFRP66L-TetR>NLS>TUP1>tABF1-pTEF2>Rho1C17R>>GAL4>tGAL4-pTEF1(TetO)>GAL80Arm2
ExpressionYeastMutationDHFR (P66L), Rho1 (C17R)Available SinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2692
Plasmid#142358PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
yeast_DualReporter
Plasmid#127546PurposeA dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).DepositorInsertsUseSynthetic BiologyExpressionYeastPromoterTEF, TEF2, URA3, bla (E. coli), and pTpA+MCSAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF2688
Plasmid#143374PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2689
Plasmid#144148PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2690
Plasmid#144149PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2691
Plasmid#142357PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTXB3-Flag PLK1
Plasmid#68271Purposebacterial expression of Plk1DepositorInsertPLK1 (PLK1 Human)
TagsFlag and Mxe intein/chitin binding domainExpressionBacterialPromoterT7Available SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTXB3-Flag PLK1 T210D
Plasmid#68272Purposebacterial expression of Plk1 T210D mutantDepositorInsertPLK1 (PLK1 Human)
TagsFlag and Mxe intein/chitin binding domainExpressionBacterialMutationT210DPromoterT7Available SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS426GFP-2×PH(PLCδ)
Plasmid#36092DepositorInsert2xPH(PLCdelta) fusion
TagsGFPExpressionYeastMutationcontains aa11-140 of PLCδ (including the PH domai…PromoterPRC1Available SinceJune 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS424GFP-2xPH(Osh2)
Plasmid#36095DepositorInsert2xPH(Osh2) fusion
TagsGFPExpressionYeastPromoterPRC1Available SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-nGFPMNase
Plasmid#187826Purposeexpress nGFPMNase protein in E coliDepositorInsertnGFPMNASE
Tagshis tagExpressionBacterialPromoterT7 PromotorAvailable SinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP1F5
Plasmid#165064PurposeCharacterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminatorDepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1> (BamHI)yEGFP>T-PGK1-T-URA3
ExpressionYeastMutationWTAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP1H5
Plasmid#165065PurposeCharacterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-T-URA3
ExpressionYeastMutationWTAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGY245
Plasmid#239812PurposeExpress H3-NLS-EGFP in filamentous fungiDepositorInsertNLS of H3
UseFungal expressionTagsEGFPPromoterTef1INAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY214
Plasmid#239805PurposeExpress EGFP and mRFP1 simultaneously in filamentous fungiDepositorInsertsEGFP
mRFP1
UseFungal expressionPromoterTef1INAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY199
Plasmid#239801PurposeExpress EGFP in filamentous fungiDepositorInsertEGFP
UseFungal expressionPromoterTef1INAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY208
Plasmid#239803PurposeExpress mCherry in filamentous fungiDepositorInsertmCherry
UseFungal expressionPromoterTef1INAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY240
Plasmid#239811PurposeExpress H3-EGFP and H2B-mRFP1 simultaneously in filamentous fungiDepositorInsertsH3
H2B
UseFungal expressionTagsEGFP and mRFP1PromoterTef1INAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY251
Plasmid#239813PurposeExpress H2B-NLS-EGFP in filamentous fungiDepositorInsertNLS of H2B
UseFungal expressionTagsEGFPPromoterTef1INAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY203
Plasmid#239802PurposeExpress GUS in filamentous fungiDepositorInsertGUS
UseFungal expressionPromoterTef1INAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY209
Plasmid#239804PurposeExpress mRFP1 in filamentous fungiDepositorInsertmRFP1
UseFungal expressionPromoterTef1INAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY219
Plasmid#239806PurposeExpress H2B-EGFP in filamentous fungiDepositorInsertH2B
UseFungal expressionTagsEGFPPromoterTef1INAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY220
Plasmid#239807PurposeExpress H3-EGFP in filamentous fungiDepositorInsertH3
UseFungal expressionTagsEGFPPromoterTef1INAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY224
Plasmid#239808PurposeExpress H2B-mRFP1 in filamentous fungiDepositorInsertH2B
UseFungal expressionTagsmRFP1PromoterTef1INAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY225
Plasmid#239809PurposeExpress H3-mRFP1 in filamentous fungiDepositorInsertH3
UseFungal expressionTagsmRFP1PromoterTef1INAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY232
Plasmid#239810PurposeExpress EGFP-P2A-mRFP1 in filamentous fungiDepositorInsertsEGFP
mRFP1
UseFungal expressionTags2APromoterTef1INAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMST-1313
Plasmid#118076PurposepTEF1-cexA-tCYC1DepositorInsertpTEF1-cexA-tCYC1
ExpressionYeastPromoterpTEF1Available SinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHBA01
Plasmid#83535PurposeConstitutive TEF1 promoter mediated expression of the UBiC geneDepositorInsertpTEF1-UBiC-CYC1t
ExpressionYeastPromoterpTEF1Available SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP
Plasmid#137763PurposeControl plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.DepositorInsertGFP
TagsHA and mycExpressionMammalianPromoterCMVAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-Keima-cb5
Plasmid#137755PurposeTargets Keima to the endoplasmic reticulum. Used to monitor ER-phagyDepositorInsertKeima-cb5
TagsGSTExpressionMammalianPromoterCMVAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-DDK
Plasmid#137764PurposeControl plasmid for immunoprecipitation of DDK/FLAG-tagged proteins. Also a good control for transfection efficiency.DepositorInsertGFP
TagsDDKExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-RFP-cb5
Plasmid#137754PurposeTargets red flourescent protein (RFP) to the endoplasmic reticulumDepositorInsertRFP-cb5
TagsGSTExpressionMammalianPromoterCMVAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
CALCOCO1 W47A-mycDDK
Plasmid#137769PurposeCALCOCO1 LIR mutantDepositorInsertCALCOCO1 W47A
TagsmycDDKExpressionMammalianMutationW47APromoterCMVAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILGFPU0 (R)
Plasmid#83562PurposeExpress yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST
ExpressionYeastPromoterTEF1Available SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (2)
Plasmid#185852PurposeTesting the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP9E3
Plasmid#185842PurposeTesting the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
ExpressionYeastMutationNoneAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
CALCOCO1 V142A-mycDDK
Plasmid#137768PurposeCALCOCO1 CLIR mutantDepositorInsertCALCOCO1 V142A
TagsmycDDKExpressionMammalianMutationV142APromoterCMVAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILFPQ13A7
Plasmid#223837PurposeExpressing mScarletI3 under the control of the TEF1 promoter in Saccharomyces cerevisiaeDepositorInsertpTEF1>mScarletI3>
ExpressionYeastPromoterTEF1Available SinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILFPQ13B7
Plasmid#223838PurposeExpressing StayGold under the control of the TEF1 promtoer in Saccharomyces cerevisiaeDepositorInsertpTEF1>StayGold>
ExpressionYeastPromoterTEF1Available SinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILFPmTagBFP2FrFAST
Plasmid#223839PurposeExpressing mTagBFP2-FrFAST under the control of the TEF1 promtoer in Saccharomyces cerevisiaeDepositorInsertpTEF1>mTagBFP2>FrFAST
ExpressionYeastPromoterTEF1Available SinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJEx411c_pelB-NbD6-Strep2
Plasmid#228897PurposePeriplasmic Expresssion of Strep-tagged anti-Zika NbD6DepositorInsertsb0379
TagsStrep2ExpressionBacterialPromoterT7Available SinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJEx411c_pelB-N32-Strep2
Plasmid#228898PurposePeriplasmic Expresssion of Strep-tagged anti-Zika NbD32DepositorInsertsb0380
TagsStrep2ExpressionBacterialPromoterT7Available SinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4D42
Plasmid#185840PurposeTesting the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
ExpressionYeastMutationNoneAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (1)
Plasmid#185851PurposeTesting the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
CALCOCO1 L140A-mycDDK
Plasmid#137767PurposeCALCOCO1 CLIR mutantDepositorInsertCALCOCO1 L140A
TagsmycDDKExpressionMammalianMutationL140APromoterCMVAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only