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Showing: 301 - 345 of 345 results

  5. hPCNA-S228I

    Plasmid
    #190938
    Purpose
    untagged human PCNA with a hypomorphic disease-associated serine to isoleucine mutation at residue 228
    Depositor
    Brian Kelch
    Article
    Duffy et al J Mol Biol. 2016 Mar 27;428(6):1023-1040
    Insert
    Proliferating Cell Nuclear Antigen (PCNA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed serine 228 to isoleucine
    Promoter
    T7
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    190938-plasmid-map-sequence-id-376466

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  8. pBP-T7_RBS

    Plasmid
    #72978
    Purpose
    T7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    T7+RBS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72978-plasmid-map-sequence-id-447502

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  9. pGCNK58

    Plasmid
    #202461
    Purpose
    Plasmid for the expression of the basic region and leucine zipper domain of the Saccharomyces cerevisiae transcription factor GCN4 in bacterial cells
    Depositor
    Arthur Palmer, Stephen Harrison
    Article
    Weiss et al Nature. 1990 Oct 11;347(6293):575-8. doi
    Insert
    Residues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension (GCN4 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    202461-plasmid-map-sequence-id-403118

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  15. pLyGo-Ec-1

    Plasmid
    #163127
    Purpose
    pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Ubiquitin
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    163127-plasmid-map-sequence-id-331491

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  19. pET-Ce25

    Plasmid
    #51296
    Purpose
    Expresses a plant arginyl-tRNA synthetase in E.coli
    Depositor
    Gabor Igloi
    Article
    Hogg et al Protein Expr Purif. 2008 Oct;61(2):163-7
    Insert
    arginyl-tRNA synthetase
    Use
    Tags
    His Tag and Thioredoxin
    Expression
    Bacterial
    Mutation
    GTT (Val) insertion after initiation codon to per…
    Promoter
    T7
    Available since
    July 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    51296-plasmid-map-sequence-id-78029

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  20. pM9-BetaGal-8C

    Plasmid
    #172489
    Purpose
    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence
    Depositor
    Siegfried Musser
    Article
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    M9-BetaGal-8C
    Use
    Tags
    6xHis and M9 nuclear localization sequence (from …
    Expression
    Bacterial
    Mutation
    BetaGal has two cysteine mutations (C77A, C1022S)…
    Promoter
    T7
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    172489-plasmid-map-sequence-id-356067

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  31. pET-Cb2

    Plasmid
    #53529
    Purpose
    Expresses an insect mitochondrial arginyl-tRNA synthetase in E.coli
    Depositor
    Gabor Igloi
    Article
    Leisinger et al Insect Biochem Mol Biol. 2013 Dec;43(12)
    Insert
    mitochondrial arginyl-tRNA synthetase
    Use
    Tags
    His Tag and Thioredoxin
    Expression
    Bacterial
    Mutation
    changed Ser4 to Ala4 to permit NcoI cloning
    Promoter
    T7
    Available since
    July 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    53529-plasmid-map-sequence-id-83364

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  36. pTK41

    Plasmid
    #236110
    Purpose
    Expresses the motor domain of rat KIF5C fused with SNAP tag and ALFA tag nanobody
    Depositor
    Shinsuke Niwa
    Article
    Kita et al Cell Rep Phys Sci, 6 (2): 102440. 19 Feb
    Inserts
    KIF5C(C7S)(1-430)-SNAP-His (Kif5c Rat)
    KIF5C(C7S)(1-430)-ALFA tag nanobody-StrepII tag (Kif5c Rat)
    Use
    Tags
    ALFA tag nanobody, His tag, SNAP tag, and StrepII…
    Expression
    Bacterial
    Mutation
    C7S mutation is introduced. 1-430 aa is encoded.
    Promoter
    T7
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236110-plasmid-map-sequence-id-472830

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Showing: 301 - 345 of 345 results