We narrowed to 3,022 results for: BFP

Showing: 121 - 140 of 3022 results

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TagBFP(S2-S4)3-gs

Plasmid

#228075

Purpose

Mammalian expression of coiled coil segments (S2-S4)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2, S4

Expression

Mammalian

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_CloningVector_tagBFP

Plasmid

#163747

Purpose

Empty gRNA cloning vector, contains a tagBFP fluorescent marker for FACS sorting after transfection.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pFH2.18_TagBFP

Plasmid

#205533

Purpose

Express TagBFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

TagBFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pKozak_mTagBFP2_synCoTC

Plasmid

#199493

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

mTagBFP2

Use

CRISPR

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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50_p5E_UBI_loxTagBFP

Plasmid

#172518

Purpose

p5E tol2kit compatible clone encoding a Ubiquitin promoter and a tagBFP sequence terminated by 3x polyA sequences and flanked by floxP sites for CRE-excision

Depositor

Jean Giacomotto

Article

Guo et al bioRxiv 2024.02.22.581357

Insert

ubiquitin promoter, TagBFP, loxP sites (inversion)

Promoter

Ubiquitin

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_EBFP2

Plasmid

#167889

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_EBFP2

Plasmid

#167865

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G3_EBFP_Nick_Dual_sgRNA

Plasmid

#178094

Purpose

Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and EBFP Nick sgRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G3 + EBFP nick sgRNAs

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pROP-PP-FBFP

Plasmid

#122137

Purpose

FMN-based fluorescent protein Reporter plasmid for B-Rex contain perfect operator sequence (anaerobic purpose)

Depositor

Srivatsan Raman

Article

Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

Insert

FBFP

Expression

Bacterial

Promoter

J23101

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-mTagBFP2

Plasmid

#122373

Purpose

Expresses mTagBFP2 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

mTagBFP2

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHR_SFFv_Her2-BFP_RHL027

Plasmid

#164823

Purpose

Lentiviral vector for constitutive expression of HER2+TM(23-675)-BFP

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Insert

Extracellular and transmembrane region of ERBB2 (ERBB2 Human)

Use

Lentiviral

Tags

tagBFP

Promoter

SFFV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCN-mBFP

Plasmid

#63847

Purpose

Encodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.

Depositor

Howard Salis

Article

Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

Insert

mBFP

Expression

Bacterial

Mutation

Promoter and RBS was swapped. His-tag was removed…

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Lysosomes-20

Plasmid

#55308

Purpose

Localization: Lysosome Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

LAMP1

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pHR_HNF1A-RE_tBFP_PGK_mCitrine

Plasmid

#188387

Purpose

Lentiviral vector - tBFP reporter for HNF1A-based SNIPRs with a constitutive mCitrine;

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

HNF1A-RE_tBFP_PGK_mCitrine

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Lifeact-7

Plasmid

#54602

Purpose

Localization: Actin, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Lifeact-mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pSpCas9_T2A_mCherry sgBFP

Plasmid

#192929

Purpose

All-in-one plasmid for cutting BFP region in DSB Spectrum V2

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Tags

T2A mCherry

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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EBFP2-H2B-6

Plasmid

#55243

Purpose

Localization: Nucleus/Histones, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

EBFP2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pBAD-mTagBFP2

Plasmid

#34632

Depositor

Vladislav Verkhusha

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

mTagBFP2

Expression

Bacterial

Mutation

I174A compared to mTagBFP (enhanced photostabilit…

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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lenti.Cas9.BFP.Blast

Plasmid

#196714

Purpose

WT SpCas9 with BSD-TagBFP for selection and sorting

Depositor

Bernhard Schmierer

Insert

Cas9-T2A-BSD-TagBFP

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Promoter

EF1a

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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EBFP2-Kindlin1

Plasmid

#242821

Purpose

Encodes wildtype human kindlin1 and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Kindlin1 (Fermt1 Human)

Expression

Mammalian

Mutation

N/A

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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