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VCP(R155H)-EGFP

Plasmid

#23972

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Use

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

R155H

Promoter

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
VCP(A232E)-EGFP

Plasmid

#23973

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Use

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

A232E

Promoter

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
VCP(DK0)-EGFP

Plasmid

#23974

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Use

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

E305QE578Q

Promoter

Available since

March 22, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-parkin W403A

Plasmid

#45876

Depositor

Edward Fon

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin (PRKN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

W403A mutation

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-UBE2QL1-GFP

Plasmid

#124667

Purpose

Expresses UBE2QL1 with C-terminal GFP tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-WRAP53beta-WD40domain

Plasmid

#64678

Purpose

Expresses the WD40 domain of WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Wd40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

containing only WD40 domain of WRAP53beta

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pGFP-mVprBP pc2953

Plasmid

#201698

Purpose

Expresses of GFP-tagged VprBP in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

VprBP (Dcaf1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-ataxin-3

Plasmid

#89975

Purpose

Expresses GFP-tagged ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-TRIM28

Plasmid

#45568

Depositor

Fanxiu Zhu

Article

Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

Insert

TRIM28 (TRIM28 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

G34S mutation in EGFP

Promoter

CMV

Available since

May 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAC150-RFP-EGFP-LGALS3 R186S

Plasmid

#175779

Purpose

PiggyBac Vector expressing GFP-RFP-LGALS3 R186S

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Tags

Expression

Mammalian

Mutation

R186S

Promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR RPS27A K113R-3xFlag_IRES-GFP

Plasmid

#198391

Purpose

RPS27A K113R (ubiquitin/ribosomal protein eS31 fusion) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RPS27A (RPS27A Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

ubiquitination site mutant (K113R)

Promoter

EF1A

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET151-GFP-PP-RPN2 (925-953)

Plasmid

#73744

Purpose

Expression of GFP-PP-RPN2 (925-953) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Use

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 932-953 of RPN2

Promoter

T7

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pET151-GFP-PP-RPN2 (940-953)

Plasmid

#73746

Purpose

Expression of GFP-PP-RPN2 (940-953) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Use

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 940-953 of RPN2

Promoter

T7

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pET151-GFP-PP-RPN2 (918-932)

Plasmid

#73748

Purpose

Expression of GFP-PP-RPN2 (918-932) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Use

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 918-932 of RPN2

Promoter

T7

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pET151-GFP-PP-RPN2 (918-953)

Plasmid

#73743

Purpose

Expression of GFP-PP-RPN2 (918-53) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Use

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 918-953 of RPN2

Promoter

T7

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pET151-GFP-PP-RPN2 (932-953)

Plasmid

#73745

Purpose

Expression of GFP-PP-RPN2 (932-953) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Use

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 932-953 of RPN2

Promoter

T7

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pET151-GFP-PP-RPN2 (918-947)

Plasmid

#73747

Purpose

Expression of GFP-PP-RPN2 (918-947) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Use

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 918-947 of RPN2

Promoter

T7

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pET151-GFP-PP-RPN2 (797-953)

Plasmid

#74228

Purpose

[Edit] Expression of GFP-PP-RPN2 (797-953) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Use

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 918-953 of RPN2

Promoter

T7

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
11K-cyto-->R GLUT1-GFP

Plasmid

#200096

Purpose

human GLUT1 with K-->R mutations for all 11 cytosolic lysines and a C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 6, 7, 225, 229,…

Promoter

CMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
UbG76V-eGFP-V5His_15aa

Plasmid

#23969

Depositor

Nico Dantuma

Article

Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

Insert

Ub-G76V-eGFP-V5HIS_15aa (UBB Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

G76V mutation in Ubiquitin. Ub fused to N-termin…

Promoter

Available since

April 16, 2010

Availability

Academic Institutions and Nonprofits only