-
Plasmid#149372PurposePromoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detectionDepositorInsertCDDR BFP Homology Donor
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti-CDDR
Plasmid#149355PurposeCRISPR/Cas9-based dual-fluorescent DSB repair reporterDepositorInsertsCDDR Reporter
Blasticidin resistance
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a and hPGKAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-Tmprss6(S762A)
Plasmid#18791DepositorInsertType II transmembrane serine protease 6 (Tmprss6 Mouse)
UseTags3xFLAGExpressionMammalianMutationChanged Serine762 to AlaninePromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Tmprss6 (delC)
Plasmid#18793DepositorInsertType II transmembrane serine protease 6 (Tmprss6 Mouse)
UseTagsGFPExpressionMammalianMutationdeleted amino acids 81-811PromoterAvailable sinceAug. 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Tmprss6 (delN)
Plasmid#18792DepositorInsertType II transmembrane serine protease 6 (Tmprss6 Mouse)
UseTagsGFPExpressionMammalianMutationdeleted amino acids 1-54PromoterAvailable sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
Cas9-sgRNA-A
Plasmid#149369PurposeExpresses SpCas9 and sgRNA targeting the lenti-CDDR reporterDepositorInsertsCas9
sgRNA-A
Puromycin resistance
UseCRISPRTags3xFLAG, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterCBh, PGK, and U6Available sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-Tmprss6(Mask)
Plasmid#18790DepositorInsertType II transmembrane serine protease 6 (Tmprss6 Mouse)
UseTags3xFLAGExpressionMammalianMutationdeleted amino acids 567-811 and replaced with 28 …PromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
Cas9-sgRNA-A+B
Plasmid#149370PurposeExpresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sitesDepositorInsertsCas9
sgRNA-A
sgRNA-B
Puromycin resistance
UseCRISPRTags3xFLAG, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterCBh, PGK, and U6Available sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Syn-ChR-tdTomato
Plasmid#99843PurposeAAV expression of ChR2(H134R)-tdTomato under the Syn promoterDepositorInsertChR2(H134R)-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChR2(H134R), carrying a single point mutation at …Promoterhuman synapsin I promoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2T-CAG-MCS-P2A-GFP-PuroR
Plasmid#107186PurposeBase plasmid for cloning gene duplication alleles with eGFP reporter.DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2T-CAG-SpCas9-BlastR
Plasmid#107190PurposeConfers constitutive expression of SpCas9DepositorInsertSpCas9 (NEWENTRY )
UseCRISPRTags3xFLAGExpressionBacterial and MammalianMutationPromoterChicken ß-actinAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2T-CAG-KKHSaCas9-BlastR
Plasmid#107189PurposeConfers constitutive expression of KKH SaCas9.DepositorInsertKKH SaCas9 (NEWENTRY )
UseTagsExpressionBacterialMutationE782K/N968K/R1015HPromoterChicken ß-ActinAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETSXM2-pLacI-mtrC
Plasmid#174615PurposeBacterial expression of mtrC from Shewanella oneidensis MR-1.DepositorInsertMtrC
UseSynthetic Biology; Shuttle vector in e. coli and …TagsExpressionBacterialMutationPromoterpLacIAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
M34 Xdsh PDZ bait-pGBT9
Plasmid#17190DepositorInsertXdsh (dvl2.L Frog)
UseTagsGal4-DBDExpressionYeastMutationDsh bp 343-1191PromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
XE160 XDshHA-pt mutant PDZ-CS2+
Plasmid#16780DepositorInsertDishevelled (dvl2.L Frog)
UseXenopus expressionTagsHAExpressionMutationpoint mutation in the binding pocket of the PDZ b…PromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
XE161 XDshHA-triple pt mutant-CS2+
Plasmid#16779DepositorInsertDishevelled (dvl2.L Frog)
UseXenopus expressionTagsHAExpressionMutationtriple point mutation outside of the PDZ binding …PromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-7xTRE-3xeGFP
Plasmid#191206PurposeGFP expression under the control of TRE promotorDepositorInsert3X GFP
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-FRT-2xtTA
Plasmid#191205PurposeFlpo dependent tTA expression under the control of TRE promotorDepositorInsert2xtTA
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC119-MCS
Plasmid#58807Purposecontains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmSod1-WT-N’His
Plasmid#117701PurposeExpression of WT mouse SOD1 with N- terminal His tagDepositorInsertSod1 (Sod1 Mouse)
UseTagsHis tag with Thrombin cleavage siteExpressionBacterialMutationPromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only