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pCMV-FLAG-PGRMC1-1-170

Plasmid

#173467

Purpose

PGRMC1 lacking the extreme C-terminus

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1 (PGRMC1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Deleted for amino acids 171-194

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-PGRMC1-1-54

Plasmid

#173472

Purpose

PGRMC1 lacking the C-terminal region downstream of the transmembrane domain

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1 (PGRMC1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Deleted for amino acids 55-194

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-PGRMC1-21-195

Plasmid

#173473

Purpose

PGRMC1 lacking the N-terminal region upstream of the transmembrane region

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1 (PGRMC1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Deleted for amino acids 1-20

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM_HcBOP1ainsitu

Plasmid

#136529

Purpose

Template for generating in situ hybridization probe

Depositor

Junko Kyozuka

Article

Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

Insert

Houttuynia cordata BLADE-ON-PETIOLE homolog1a

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM_ZmaBOP1binsitu

Plasmid

#136531

Purpose

Template for generating in situ hybridization probe

Depositor

Junko Kyozuka

Article

Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

Insert

Zoysia matrella BLADE-ON-PETIOLE homolog1b

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM_ZmaBOP2binsitu

Plasmid

#136532

Purpose

Template for generating in situ hybridization probe

Depositor

Junko Kyozuka

Article

Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

Insert

Zoysia matrella BLADE-ON-PETIOLE homolog2b

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM_HcBOP1binsitu

Plasmid

#136530

Purpose

Template for generating in situ hybridization probe

Depositor

Junko Kyozuka

Article

Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

Insert

Houttuynia cordata BLADE-ON-PETIOLE homolog1b

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PB533-42B3mutAC2-sfGFP

Plasmid

#186777

Purpose

RNAP2 Ser2ph-mintbody [42B3(R78K/A80T/M95V)-scFv] expression vector for mammalian cells

Depositor

Hiroshi Kimura

Article

Uchino et al J Cell Biol. 2022 Feb 7;221(2). pii: 212

Insert

42B3mutAC2-scFv

Use

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCold-TF-hRubicon

Plasmid

#64147

Purpose

For protein expression in E. coli

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

Rubicon (RUBCN Human)

Use

Tags

6xHis-tag and Trigger Factor (TF)

Expression

Bacterial

Mutation

Promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
plenti-CMV-mCherry-T2A-GFP

Plasmid

#109427

Purpose

A lentiviral backbone expressing mCherry-T2A-eGFP off of a CMV promoter.

Depositor

Reuben Harris

Article

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

mCherry T2A GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-UBC-TagRFP-T-ps

Plasmid

#178656

Purpose

Expression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cells

Depositor

Jeffrey Gerst

Article

Haimovich et al Proc Natl Acad Sci U S A. 2017 Nov 14;11

Insert

TagRFP-T with palmitoylation signal at N-terminus

Use

Lentiviral

Tags

Palmitoylation signal

Expression

Mutation

Promoter

Ubc

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCold-TF-hPLEKHM1

Plasmid

#64146

Purpose

For protein expression in E.coli

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

PLEKHM1 (PLEKHM1 Human)

Use

Tags

6xHis-tag and Trigger Factor (TF)

Expression

Bacterial

Mutation

Promoter

cspA

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
S12.1_Empty_Bridge

Plasmid

#127670

Purpose

Ampicillin resistant multiplexing backbone without CRY2HA and mCherry

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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S12.2_Bridge + Target

Plasmid

#127671

Purpose

Ampicillin resistant multiplexing backbone with CRY2HA and mCherry

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Empty Anchor Control

Plasmid

#127665

Purpose

Encodes for puromycin resistance expressed from the EF1a promoter

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

Puro

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-PGRMC-Hybrid1

Plasmid

#173469

Purpose

N-terminal region of PGRMC2 fused to the transmembrane and C-terminal region of PGRMC1

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG-PGRMC-Hybrid2

Plasmid

#173470

Purpose

N-terminal and transmembrane region of PGRMC2 fused to the C-terminal region of PGRMC1

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG-PGRMC-Hybrid3

Plasmid

#173471

Purpose

N-terminal region of PGRMC1 fused to the transmembrane and C-terminal region of PGRMC2

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-hPLEKHM1

Plasmid

#64145

Purpose

For yeast two hybrid

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

PLEKHM1 (PLEKHM1 Human)

Use

Tags

GAL4 activation domain

Expression

Yeast

Mutation

Promoter

ADH

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pGAD-hRubicon

Plasmid

#64144

Purpose

For yeast two hybrid

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

Rubicon (RUBCN Human)

Use

Tags

GAL4 activation domain

Expression

Yeast

Mutation

Promoter

ADH1

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only