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Showing: 121 - 140 of 188 results

  6. pBFC0984

    Plasmid
    #231992
    Purpose
    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Inserts
    dRfxCas13d
    crRNA with 2xBsaI spacer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 and pTet
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231992-plasmid-map-sequence-id-467618

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  7. pBFC0993

    Plasmid
    #231993
    Purpose
    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Inserts
    dRfxCas13d
    crRNA with negative control (RFP-targeting) spacer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 and pTet
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231993-plasmid-map-sequence-id-467616

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  8. TC1316

    Plasmid
    #164883
    Purpose
    CMV-bpNLS-dCas13d-bpNLS-ADARdd-mCherry, ADARdd embeded in loop3
    Depositor
    Tian Chi
    Article
    Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:
    Insert
    dcas13d-ADARdd
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dCas13d L560-G586 deleted
    Promoter
    CMV, T7
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    164883-plasmid-map-sequence-id-325957

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  9. pBFC1171

    Plasmid
    #231994
    Purpose
    CRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning site
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Insert
    crRNA of dRfxCas13d
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJEx (Jungle Express)
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    231994-plasmid-map-sequence-id-468506

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  12. pAAV-U6-DR30(SapI) 0.5 Syn-intron-CasRx-pA

    Plasmid
    #233038
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a 0.5 bp synapsin promoter
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and CasRx
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233038-plasmid-map-sequence-id-463774

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  13. pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA

    Plasmid
    #233045
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and CasRx and mCherry
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233045-plasmid-map-sequence-id-467425

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  17. hfDECOR-F148A

    Plasmid
    #219546
    Purpose
    Expression of CMV-TadA8e-F148A-NLS-dCas13d-NLS in mammalian cells (hfDECOR-F148A expression)
    Depositor
    Weixin Tang
    Article
    Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/
    Insert
    hfDECOR-F148A
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Changed TadA8e Phenylalanine 148 to Alanine
    Promoter
    CMV
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    219546-plasmid-map-sequence-id-443106

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  18. hfDECOR-V106G

    Plasmid
    #219547
    Purpose
    Expression of CMV-TadA8e-V106G-NLS-dCas13d-NLS in mammalian cells (hfDECOR-V106G expression)
    Depositor
    Weixin Tang
    Article
    Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/
    Insert
    hfDECOR-V106G
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Changed TadA8e Valine 106 to Glycine
    Promoter
    CMV
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    219547-plasmid-map-sequence-id-443107

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  20. pRC065d

    Plasmid
    #225979
    Purpose
    dRxCas13d expressed from J23107 promoter with J23119-driven crRNA (non-cognate DR)
    Depositor
    Jesse Zalatan
    Article
    Cardiff et al Nucleic Acids Res. 2024 May 22;52(9):540
    Inserts
    dRxCas13d
    buj1 crRNA (non-cognate DR) targets the unstructured RBS
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    J23107 and J23119
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    225979-plasmid-map-sequence-id-448802

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Showing: 121 - 140 of 188 results