We narrowed to 1,247 results for: membrane gfp

1. Hook1-EGFP

   Plasmid

   #171941

   Purpose

   Expresses Hook1 in mammalian cells

   Depositor

   Cheng-han Yu

   Article

   Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   Hook1
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTK50_GFP-NuMA-N

   Plasmid

   #46348

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   NuMA N-terminal fragment (NUMA1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N-terminal fragment from 1-213

   Promoter

   pBABE

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTK51_GFP-NuMA-C

   Plasmid

   #46349

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   NuMA C-terminal fragment (NUMA1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C-terminal fragment from 1700-2115

   Promoter

   pBABE

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. UBQ10:sXVE:GFP1–10

   Plasmid

   #125663

   Purpose

   Binary vector for the inducible expression of GFP1–10 fragment that can be used to detect a protein tagged with GFP11

   Depositor

   Tzu-Yin Liu

   Article

   Liu et al Methods Mol Biol. 2021;2200:323-336. doi

   Insert

   GFP1–10
   

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   UBQ10:sXVE:

   Available Since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-ShadowY

   Plasmid

   #193555

   Purpose

   Expresses mEGFP-ShadowY in mammalian cells.

   Depositor

   Justin Taraska

   Article

   Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

   Insert

   ShadowY
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP VAMP7 (102-220)

   Plasmid

   #42314

   Depositor

   Thierry Galli

   Article

   Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

   Insert

   VAMP7 Coiled Coil domain (102-220) (VAMP7 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Δ1-102

   Promoter

   CMV

   Available Since

   March 13, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pQE80L-SpyCatcher-ELP-GFP

   Plasmid

   #69835

   Purpose

   Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

   Insert

   SpyCatcher-ELP-GFP
   

   Tags

   6x His Tag, GFP, and TEV Tag

   Expression

   Bacterial

   Promoter

   T5 promoter/lac operator element

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. PP_080_pAAV_CAG_DIO-LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

   Plasmid

   #230990

   Purpose

   Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

   Depositor

   Adam Cohen

   Article

   Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

   Insert

   Cre-dependent, membrane-localized Voltron2 and soma-localized CheRiff
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-SNAP-CD86tm-eGFP-FKBP(f36v)

   Plasmid

   #200280

   Purpose

   Membrane protein fusion with extracellular SNAPtag and intracellular FKBP(f36v) dimerization domain and GFP

   Depositor

   Kristina Ganzinger

   Article

   Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

   Insert

   SNAP-tag-CD86tm-eGFP-FKBP(f36v)
   

   Use

   Lentiviral

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-eGFP-CD86tm-HALOtag-FKBP(f36v)

    Plasmid

    #200281

    Purpose

    Membrane protein fusion with extracellular GFP and intracellular FKBP(f36v) dimerization domain and HALOtag

    Depositor

    Kristina Ganzinger

    Article

    Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

    Insert

    eGFP-CD86tm-HaloTag-FKBP
    

    Use

    Lentiviral

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. cmamxA1-GFP

    Plasmid

    #107195

    Purpose

    Expresses porcine annexin A1-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

    Depositor

    Volker Gerke, Ursula Rescher

    Article

    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

    Insert

    cmannexin A1
    

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    exchanges Asp170Ala, Glu255Ala, Glu330Ala, all ty…

    Promoter

    CMV

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-INPP5E-CAAX

    Plasmid

    #202746

    Purpose

    express plasma membrane targeted fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    EGFP:INPP5E(214-644):HRAS(172-189) (HRAS, INPP5E Human)
    

    Expression

    Mammalian

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSG66-KcsA-Kv1.3-sfGFP

    Plasmid

    #102457

    Purpose

    Expresses a fusion protein of KcsA-Kv1.3 and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.

    Depositor

    Richard Murray

    Article

    Guo et al bioRxiv 104455

    Insert

    KcsA-Kv1.3
    

    Tags

    His tag and sfGFP

    Expression

    Bacterial

    Promoter

    OR2-OR1-Pr

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Venus-7aa-MAO-pEGFP-C3

    Plasmid

    #166765

    Purpose

    To target Venus to the mitochondrial outer membrane. MAO tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.

    Depositor

    David Andrews

    Insert

    MAO tail anchor (MAOA Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSG69-KcsA-Kv1.3-deGFP

    Plasmid

    #102458

    Purpose

    Expresses KcsA-Kv1.3 protein with a C-terminal His tag and a deGFP fluorescent protein with a different ribosome binding site.

    Depositor

    Richard Murray

    Article

    Guo et al bioRxiv 104455

    Inserts

    KcsA-Kv1.3
    deGFP
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    OR2-OR1-Pr

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax

    Plasmid

    #190153

    Purpose

    Expresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

    Depositor

    Brad Zuchero

    Article

    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

    Insert

    Vamp2 (Vamp2 Mouse)
    

    Use

    AAV

    Tags

    P2AT2A-EGFP-caax

    Expression

    Mammalian

    Mutation

    Truncation that includes only AA #1-94

    Promoter

    MBP

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax

    Plasmid

    #190154

    Purpose

    Expresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

    Depositor

    Brad Zuchero

    Article

    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

    Insert

    Vamp3 (Vamp2 Rat)
    

    Use

    AAV

    Tags

    P2AT2A-EGFP-caax

    Expression

    Mammalian

    Mutation

    Truncation that includes only rat Vamp3 AA #1-81

    Promoter

    MBP

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS424GFP-2xPH(Osh2)

    Plasmid

    #36095

    Depositor

    Scott Emr

    Article

    Stefan et al Cell. 2011 Feb 4;144(3):389-401.

    Insert

    2xPH(Osh2) fusion
    

    Tags

    GFP

    Expression

    Yeast

    Promoter

    PRC1

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-UbC-eGFP-F

    Plasmid

    #71545

    Purpose

    Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.

    Depositor

    Pantelis Tsoulfas

    Insert

    eGFP-F
    

    Use

    AAV

    Tags

    contains 20- amino-acid farnesylation signal from…

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTK64_GFP-NuMA-Bonsai

    Plasmid

    #46351

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    NuMA deleted 414-1549 (NUMA1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acid 414-1549; L1589F mutation in N…

    Promoter

    pBABE

    Available Since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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