Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 177 results for: pcna

Showing: 121 - 140 of 177 results

  2. pGEX6P1‐Afu‐rnhBΔPIP

    Plasmid
    #108696
    Purpose
    For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletion
    Depositor
    Martin Reijns, Andrew Jackson
    Article
    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6
    Insert
    rnhB
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    C-terminal truncation (7aa) removing PIP box
    Promoter
    tac
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    108696-plasmid-map-sequence-id-208999

    Enlarge

  3. pgRNA_CV2

    Plasmid
    #167165
    Purpose
    pgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.
    Depositor
    Alexander Loewer
    Article
    Sheng et al Cell Rep. 2019 Apr 2;27(1):48-58.e7. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter
    Available since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167165-plasmid-map-sequence-id-330265

    Enlarge

  11. pR1G108CP20X

    Plasmid
    #85086
    Purpose
    Expresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    G108C in PCNA1
    Promoter
    T7 promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    85086-plasmid-map-sequence-id-164463

    Enlarge

  12. pPD3C

    Plasmid
    #86971
    Purpose
    Expresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA3 fused to phosphite dehydrogenase and P450cam
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, N106R in PCNA3, silent mutat…
    Promoter
    T7 promoter
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86971-plasmid-map-sequence-id-173928

    Enlarge

  13. pPD1cR

    Plasmid
    #86970
    Purpose
    Expresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, G108C in PCNA1
    Promoter
    T7 promoter
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86970-plasmid-map-sequence-id-173927

    Enlarge

  14. pPD2cX

    Plasmid
    #86968
    Purpose
    Expresses PCNA2 variant (L171C) fused phosphite dehydrogenase and putidaredoxin in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, C73S/C85S in PdX, L171C in P…
    Promoter
    T7 promoter
    Available since
    March 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    86968-plasmid-map-sequence-id-173869

    Enlarge

  16. p3R112C/T180CBM3

    Plasmid
    #85090
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The R112C/T180C variant of PCNA3 fused to P450 BM3
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3
    Promoter
    T7 promoter
    Available since
    Dec. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    85090-plasmid-map-sequence-id-164484

    Enlarge

  17. pPD3cC

    Plasmid
    #86972
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …
    Promoter
    T7 promoter
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86972-plasmid-map-sequence-id-173929

    Enlarge

  18. p1G108CFa

    Plasmid
    #85089
    Purpose
    Expresses PCNA1 variant (G108C) fused to an FAD domain of P450 BM3 (A74G/C773S/C810S) in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The G108C variant of PCNA1 fused to an FAD domain of P450 BM3
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    G108C in PCNA1, A74G/C773S/C810S in FAD domain of…
    Promoter
    T7 promoter
    Available since
    Nov. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    85089-plasmid-map-sequence-id-164483

    Enlarge

  19. p3R112C/T180CHFm

    Plasmid
    #85091
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused to heme-FMN domain of P450 BM3 variant (A74G) in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The R112C/T180C variant of PCNA3 fused to Heme-FMN domain of P450 BM3
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    R112C/T180C in PCNA3, A74G in heme-FMN domain of …
    Promoter
    T7 promoter
    Available since
    Dec. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    85091-plasmid-map-sequence-id-164485

    Enlarge

Showing: 121 - 140 of 177 results