We narrowed to 128 results for: zeocin resistance

1. pFUGW-shRIIα-RIIαS97A-IRES-EGFP

   Plasmid

   #183457

   Purpose

   Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA and Prkar2a (Prkar2a Rat)
   

   Use

   Lentiviral

   Mutation

   shRNA-resistant mutations: C135>G, G138>A, …

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUGW-shRIIα-RIIαS97E-IRES-EGFP

   Plasmid

   #183458

   Purpose

   Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA and Prkar2a (Prkar2a Rat)
   

   Use

   Lentiviral

   Mutation

   shRNA-resistant mutations: C135>G, G138>A, …

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDNA5-FRT/TO-FLAG-TONSL

   Plasmid

   #221011

   Purpose

   mammalian expression vector of Flag tagged TONSL siRNA resistant

   Depositor

   Daniel Durocher

   Article

   O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

   Insert

   TONSL (TONSL Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   silent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…

   Promoter

   CMV

   Available Since

   June 24, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. lenti-EF1a-dCas9-VPR-Puro

   Plasmid

   #99373

   Purpose

   3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   (Sp)dCas9-VPR-P2A-Puro
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

   Plasmid

   #166133

   Purpose

   This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

   Insert

   hU6-gRNA and hH1-gRNA targeting cldn1 gene
   

   Use

   CRISPR and Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro

   Plasmid

   #166134

   Purpose

   This plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

   Insert

   hU6-gRNA and hH1-gRNA targeting firefly luciferase gene
   

   Use

   CRISPR and Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 Myc2- Hs TTP WT

   Plasmid

   #107008

   Purpose

   Expresses epitope-tagged wild-type human TTP

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   tristetraprolin (ZFP36 Human)
   

   Tags

   Double Myc peptide epitope tag

   Expression

   Mammalian

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC:FCP:ShBle:FCP:EGFP

   Plasmid

   #85987

   Purpose

   Expresses ShBle and EGFP with FCP promoters/terminators for F. cylindrus transformation.

   Depositor

   Thomas Mock

   Article

   Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

   Inserts

   ShBle
   EGFP
   

   Promoter

   FCP

   Available Since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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