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Addgene
Showing: 121 - 140 of 12744 results
  1. pAC1-pCR8-dCas9VP48

    Plasmid
    #48214
    Purpose
    dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Insert
    dCas9(D10A;H840A) fusion with VP48 activation domain
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag and VP48
    Expression
    Mutation
    D10A;H840A
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pAC147-pCR8-dCas9VP64

    Plasmid
    #48219
    Purpose
    dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression 
    Depositor
    Insert
    dCas9(D10A;H840A) fusion with VP64 activation domain
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag and VP64
    Expression
    Mutation
    D10A;H840A
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pAC148-pCR8-dCas9VP96

    Plasmid
    #48220
    Purpose
    dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Insert
    dCas9(D10A;H840A) fusion with VP96 activation domain
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag and VP96
    Expression
    Mutation
    D10A;H840A
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pAC149-pCR8-dCas9VP160

    Plasmid
    #48221
    Purpose
    dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag and VP160
    Expression
    Mutation
    D10A;H840A nuclease-deficient
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pAC95-pmax-dCas9VP160-2A-neo

    Plasmid
    #48227
    Purpose
    dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.
    Depositor
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
    Use
    CRISPR
    Tags
    2A-neo, HA Tag, and VP160
    Expression
    Mammalian
    Mutation
    D10A;H840A (catalytically inactive)
    Promoter
    CAGGS
    Availability
    Academic Institutions and Nonprofits only
Showing: 121 - 140 of 12744 results