We narrowed to 18,056 results for: puro

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pLentiCRISPR v2 puro mGSDME gRNA1

Plasmid

#223521

Purpose

Knocking out mGSDME in mouse cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (GSDME Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro

Plasmid

#195567

Purpose

PiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cells

Depositor

Joanna Wysocka

Article

Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

Insert

TIR1

Expression

Mammalian

Promoter

Ef1-alpha

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-TRE-miRFP703_PGK-Puro

Plasmid

#216667

Purpose

Expresses tTa-inducible miRFP703, with puromycin resistance

Depositor

Leonardo Morsut

Inserts

miRFP703
PuroR

Use

Lentiviral

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL2368 MTK PB puro backbone

Plasmid

#226673

Purpose

Empty MTK PB cargo backbone marked with puromycin resistance.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pYX-N-Puro-mNeon-TSC22D1

Plasmid

#222667

Purpose

endogenously tag TSC22D1 with N-terminal mNeon

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D1 (TSC22D1 Human)

Tags

mNeon

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1a-Ccswap

Plasmid

#225460

Purpose

Express EGFP-fusion protein of FXR1 isoform a (CCswap)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

CCswap (swapped positions of CC2 and CC1)

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-R rich

Plasmid

#225480

Purpose

Express mEGFP-GAPDH-Rrich fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with R-rich region of TDRD3 (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1a-I304N

Plasmid

#225461

Purpose

Express mEGFP-fusion protein of FXR1 isoform a (I304N)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

I304N

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-Tudor

Plasmid

#225479

Purpose

Express mEGFP-GAPDH-Tudor fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with Tudor domain of TDRD3 (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-RGG

Plasmid

#225477

Purpose

Express mEGFP-GAPDH-RGG fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with RGG domain of TOP3B (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro 3XFLAG-hATXN7L3

Plasmid

#225725

Purpose

Transfection of ATXN7L3 with 3XFLAG tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

ATXN7L3 (ATXN7L3 Human)

Tags

3XFLAG

Expression

Mammalian

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X S404N

Plasmid

#225724

Purpose

Transfection of USP27X (S404N) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X S404N (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

S404N

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X F313V

Plasmid

#225722

Purpose

Transfection of USP27X (F313V) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X F313V (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

F313V

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X K181E

Plasmid

#225721

Purpose

Transfection of USP27X (K181E) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X K181E (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

K181E

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X Y144C

Plasmid

#225720

Purpose

Transfection of USP27X (Y144C) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X Y144C (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

Y144C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X G76S

Plasmid

#225719

Purpose

Transfection of USP27X (G76S) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X G76S (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

G76S

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR_NPM2-T2A-mGreenLantern-PuroTK

Plasmid

#222910

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to NPM2.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR_FIGLA-T2A-mGreenLantern-PuroTK

Plasmid

#222905

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to FIGLA.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-tet2puro-gal4DBD-HIFCAD

Plasmid

#207173

Purpose

Dox Inducible Expression Plasmid for gal4DBD-HIF-CAD

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

Gal4DBD-HIF1a-CAD

Use

Lentiviral; Dox inducible

Tags

2x Myc

Expression

Mammalian

Promoter

TRE3G

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-GFP-2A-Puro-gATRIP_A

Plasmid

#211537

Purpose

sgRNA-A against ATRIP

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ATRIP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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p21(RxL)-mCerulean-PuroR

Plasmid

#215078

Purpose

Expresses p21(RxL)-mCerulean in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Use

Retroviral

Tags

mCerulean

Expression

Mammalian

Mutation

p21(RxL) denotes mutations: R19A, R20A, L21A, F22A

Promoter

LTR

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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p21(dPCNA)-mCerulean-PuroR

Plasmid

#215079

Purpose

Expresses p21(dPCNA)-mCerulean in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Use

Retroviral

Tags

mCerulean

Expression

Mammalian

Mutation

p21(dPCNA) denotes mutations M147A, D149A, F150A.

Promoter

LTR

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(CDK6-E1(6))-PGKpuro2ABFP-W

Plasmid

#200487

Purpose

Lentiviral vector expressing gRNA targeting human CDK6-E1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CDK6-E1(6) (CDK6 Human)

Use

Lentiviral

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(CXCR4-E1(15))-PGKpuro2ABFP-W

Plasmid

#200478

Purpose

Lentiviral vector expressing gRNA targeting human CXCR4-E1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CXCR4-E1(15) (CXCR4 Human)

Use

Lentiviral

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(CXCR4-E2(38))-PGKpuro2ABFP-W

Plasmid

#200481

Purpose

Lentiviral vector expressing gRNA targeting human CXCR4-E2

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CXCR4-E2(38) (CXCR4 Human)

Use

Lentiviral

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-BCL9

Plasmid

#202662

Purpose

Tet-ON 3rd generation expression vector with inducible expression of BCL9 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

BCL9 transcription coactivator (BCL9 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9HF1-P2A-puro

Plasmid

#214357

Purpose

Expresses SpCas9HF1 and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SpCas9HF1 and puromycin resistance genes

Expression

Mammalian

Mutation

N497A/R661A/Q695A/Q926A

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT4_short_mCherry2-NLS-PEST_Puro

Plasmid

#213774

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT4_short_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT4_mCherry2-NLS-PEST_Puro

Plasmid

#213773

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT4_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT3_short_mCherry2-NLS-PEST_Puro

Plasmid

#213772

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT3_short_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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