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pLL3.7m-rsGreenF-E-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-Skylan62A-2A-YFP-SLBP
Plasmid#205762PurposeSingle-color FUCCI4 senor for transitions of all four cell cylce phasesDepositorInsertrsGFE-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-SK62A-2A-YFP-SLBP
UseLentiviralTagsExpressionMammalianMutationwtPromoterAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
8522-M31-663
Plasmid#225661PurposeLentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (red configuration)DepositorInsertdsCherry
UseLentiviralTagsExpressionMutationPromoterSORE6-mCMVpAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCA14-pHR-SFFV-GFP-RPA43
Plasmid#199445PurposeExpression of GFP-RPA43 in mammalian cellsDepositorInsertRPA43 (POLR1F Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterSFFVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 HaloTag-V5-TurboID
Plasmid#226474PurposeExpresses fusion of HaloTag and TurboID in mammalian cellsDepositorInsertTurboID
UseTagsHaloTag and V5ExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEM-Nanog
Plasmid#16351DepositorInsertHomo sapiens Nanog homeobox (NANOG Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-Halo
Plasmid#175296PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with HaloTag
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Integrin β8-GFP
Plasmid#205093PurposeExpress GFP-tagged human integrin β8 in mammalian cellsDepositorInsertIntegrin beta 8 (ITGB8 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-JNKKTRrsGreenF-E-2A-ERKKTRrsFastLime-IRES-PKAKTRClover-2A-P38KTRDronpa
Plasmid#205766PurposeExpression of JNK KTR rsGreenF-E, ERK KTR rsFastLime, PKA KTR Clover, and P38 KTR Dronpa in mamalian cellsDepositorInsertJNK KTR rsGreenF-E-2A-ERK KTR rsFastLime-IRES-PKA KTR Clover-2A-P38 KTR Dronpa
UseAAVTagsExpressionMammalianMutationwtPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
HypoxCR
Plasmid#59946PurposeA lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.DepositorInsertsGFP-Pest
mCherry-Geminin
UseLentiviralTagsFLAG and PESTExpressionMammalianMutationPromoterCMV and min CMVAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
Integrin β5-GFP
Plasmid#205090PurposeExpress GFP-tagged human integrin β5 in mammalian cellsDepositorInsertIntegrin beta 5 (ITGB5 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
YFP-S100A10
Plasmid#107200PurposeExpresses YFP-human S100A10 fusion protein for live cell imagingDepositorInsertS100A10 (S100A10 Human)
UseTagsYellow Fluorescent ProteinExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pAS_4xMmaPylT(UUA)_EF1_MmaPylRS
Plasmid#174896PurposeMma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsExpressionMammalianMutationPromoterEF1Available sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
PECAM1-bio-His
Plasmid#51624PurposeExpresses full-length Platelet endothelial cell adhesion molecule precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPECAM1 (PECAM1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDNR-SpCas9-Gem
Plasmid#80424PurposeUsed for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joiningDepositorInsertSpCas9-Gem
UseCRISPRTagsGemininExpressionMutationcodon optimized for humanPromoterT7Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-SNAP
Plasmid#175295PurposeTrack mature FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with SNAP-tag
UseTagsSNAP-tagExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCA12-pHR-SFFV-BFP-B23
Plasmid#199443PurposeExpression of BFP-B23 in mammalian cellsDepositorInsertB23 (NPM1 Human)
UseLentiviralTagsTagBFPExpressionMammalianMutationPromoterSFFVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pECE M2-SNX17 WT
Plasmid#69811PurposeExpresses M2-SNX17 in mammalian cellsDepositorInsertSorting nexin-17 (SNX17 Human)
UseTagsFLAGExpressionMammalianMutationPromoterSV40 early promoterAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Gfi1b
Plasmid#131611Purposeexpression of mouse Gfi1b in mammalian cellsDepositorInsertGfi1b (Gfi1b Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Meis1
Plasmid#131605Purposeexpression of mouse Meis1 in mammalian cellsDepositorInsertMeis1 (Meis1 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCXLE-hUL + mTAGBFP2
Plasmid#74944PurposeDosage control and tracing of reprogramming episomesDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe WT Akt2
Plasmid#14551DepositorInsertAkt2 (AKT2 Human)
UseRetroviralTagsHA tagExpressionMammalianMutationPromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgMyc_1
Plasmid#138324PurposeExpress guide RNA 2 for mouse MycDepositorInsertcMyc sgRNA (Myc Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-RR-DSCP
Plasmid#83339Purposefor Gateway cloning of enhancer elements upstream of the DSCP and mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsmCherry::Renilla(nls)ExpressionInsectMutationPromoterDrosophila Synthetic Core PromoterAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
AMA1-bio
Plasmid#47741PurposeExpresses enzymatically monobiotinylated full-length AMA1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised AMA1
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSP10-bio
Plasmid#47719PurposeExpresses enzymatically monobiotinylated full-length MSP10 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSP10
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV neo WT Akt1
Plasmid#14549DepositorInsertAkt1 (Akt1 Mouse)
UseRetroviralTagsHA tagExpressionMammalianMutationPromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pHAGE2-TetOminiCMV-cMyc
Plasmid#136614PurposeDox-inducible lentiviral vector expressing mouse cMycDepositorInsertcMyc (Myc Mouse)
UseLentiviralTagsExpressionBacterialMutationPromotertetO-miniCMV (dox-inducible)Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgMyc_2
Plasmid#138346PurposeExpress guide RNA 1 for mouse MycDepositorInsertcMyc sgRNA (Myc Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTRAP-bio
Plasmid#47746PurposeExpresses enzymatically monobiotinylated full-length MTRAP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MTRAP
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
EBA140-bio
Plasmid#47742PurposeExpresses enzymatically monobiotinylated full-length EBA140 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised EBA140
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pB-TAG-HES7
Plasmid#130935PurposePiggyBac vector for DOX-inducible human HES7-IRES-EGFP expression in mammalian cellsDepositorInsertHES7 (HES7 Human)
UseTagsExpressionMammalianMutationPromoterTetOAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-Kir2.1
Plasmid#125578PurposeCre-dependent expression of Kir2.1 (FLAG-tagged) in mammalian cells.DepositorInsertKir2.1 (Kcnj2 Mouse)
UseCre/LoxTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xEcoLeuT(CUA)_EF1_AnapRS
Plasmid#174894PurposeAnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertAnapRS
UseTagsExpressionMammalianMutationL38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…PromoterEF1Available sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-GL-DSCP
Plasmid#83338Purposefor Gateway cloning of enhancer elements upstream of the DSCP and GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsGFP::Luciferase(nls)ExpressionInsectMutationPromoterDrosophila Synthetic Core PromoterAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only
