We narrowed to 5,458 results for: pcDNA3.1+

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pcDNA3.1/hChR2(H134R)-EYFP

Plasmid

#20940

Purpose

Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/opto-b2AR-EYFP

Plasmid

#20948

Purpose

Mammalian expression of opto-b2AR fused to EYFP for optical control of GPCR signaling

Depositor

Karl Deisseroth

Article

Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

Insert

opto-b2AR

Expression

Mammalian

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag-HIF1B (#930)

Plasmid

#99916

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Chen et al Nature. 2016 Nov 3;539(7627):112-117. do

Insert

HIF1B (ARNT Human)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-DDR1-F866Y-V5/HIS

Plasmid

#236009

Purpose

expression of the F866Y kinase defective mutant variant of human DDR1 that might be associated with lung Adenocarcinoma

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human DDR1-F866Y receptor tyrosine kinase, full length (DDR1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

F866Y substitution

Promoter

CMV

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

Plasmid

#117484

Purpose

Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

SpyCatcher002-6aa-sfCherry2(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)GαsL-260-NLuc

Plasmid

#207158

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAS (GNAS Human)

Expression

Mammalian

Mutation

NLuc inserted at position D(260)N

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HLA-Flag-natT1R3

Plasmid

#113950

Purpose

mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R3 (TAS1R3 Human)

Tags

FLAG and Signal/leader sequence from HLA class I …

Expression

Mammalian

Mutation

residues 21-852

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HNF1B-WT-Flag-UTR

Plasmid

#183239

Purpose

Expression of FLAG tagged HNF1B

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

HNF1 homeobox B (HNF1B Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUG-DAP5-3XFLAG

Plasmid

#127331

Purpose

Express 3xFLAG tagged DAP5 protein from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

DAP5 (EIF4G2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs

Plasmid

#86828

Purpose

Expression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequence

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hygro(+)-PSTCD-cGAS

Plasmid

#102605

Purpose

Mammalian expression of cGAS fused to PSTCD tag

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

cGAS (CGAS Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)

Plasmid

#49077

Purpose

Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

Depositor

Biff Forbush

Article

Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

Insert

human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)

Tags

3x HA and mCerulean

Expression

Mammalian

Mutation

262 silent mutations from native hNKCC1

Promoter

CMV

Available Since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hAsCpf1(TATV) (pY220)

Plasmid

#89353

Purpose

Expresses humanized AsCpf1 variant that recognizes TATV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RVR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K548V/N552R

Promoter

CMV

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-K370A-L3F6H

Plasmid

#177771

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K370A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

K370A

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 (-) + codon optimized Brn3a

Plasmid

#86829

Purpose

mammalian expression of the transcription factor Brn3a

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

Brn3a (POU4F1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5AdeltaSIV GFP Nter

Plasmid

#145770

Purpose

expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

Depositor

Hugues Abriel

Article

Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Tags

GFP

Expression

Mammalian

Mutation

Stop codon at Serine 2013

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CibN-dCas9-CibN

Plasmid

#60553

Purpose

Expresses CibN-dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CDK6

Plasmid

#172614

Purpose

Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK6 (CDK6 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gαq-124-NLuc

Plasmid

#207153

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAQ (GNAQ Human)

Expression

Mammalian

Mutation

NLuc inserted at position F(124)E

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gγ2-LSS-mKATE2(N)

Plasmid

#207160

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNA

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNG2 (GNG2 Human)

Expression

Mammalian

Mutation

N-terminally tagged with LSS-mKATE2

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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