We narrowed to 5,538 results for: pcas

1. PX458-3xHA-dSpCas9

   Plasmid

   #201953

   Purpose

   Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-dSpCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A and H840A on SpCas9

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-spCas9-pZeo

   Plasmid

   #214121

   Purpose

   Gene expression by Piggybac transposase in mammalian cells

   Depositor

   Sho Yoshimatsu

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for mammalian cells

   Promoter

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-HaloTag-KRas4B(CT)

   Plasmid

   #214276

   Purpose

   Mammalian expression of HaloTag fused to the C-terminal tail of KRas4B

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   HaloTag7-KRas4B(174-188) (KRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458-tRNA-SpCas9

   Plasmid

   #195183

   Purpose

   Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.

   Depositor

   Stephanie Roessler

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   tRNA-Gln

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. eSpCas9(1.1)_NoFlag_ATP1A1_G3_SLBP_G1_Dual_sgRNA

   Plasmid

   #191528

   Purpose

   Vector for tandem expression of SLBP 3'UTR G1 sgRNA in combination with ATP1A1 G3 sgRNA from two independent U6 promoters to facilitate SLBP endogenous tagging by marker free coselection using ouabain

   Depositor

   Yannick Doyon

   Insert

   SLBP 3'UTR G1 sgRNA + ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via hdr using ouabain

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCaUra3-CUbo-yeGFP

   Plasmid

   #212771

   Purpose

   C-terminal PCR-based tagging with CUbo-yeGFP in budding yeast with CaUra3 selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-TSF-ATG101

   Plasmid

   #210858

   Purpose

   Mammalian expression of ATG101

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG101 (ATG101 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGIG-FLAG-SHTN1L

   Plasmid

   #209568

   Purpose

   Mammalian expression of Shtn1 long isoform

   Depositor

   Sika Zheng

   Article

   Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

   Insert

   Shtn1 long isoform (4930506M07Rik Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGIG-FLAG-SHTN1S

   Plasmid

   #209569

   Purpose

   Mammalian expression of Shtn1 short isoform

   Depositor

   Sika Zheng

   Article

   Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

   Insert

   Shtn1 short isoform (4930506M07Rik Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG/ATP8B4(E170Q)-HA

    Plasmid

    #209243

    Purpose

    Mammalian expression of ATP8B4 mutant E170Q

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP8B4 (ATP8B4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E170Q

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG/ATP10B(E210Q)-HA

    Plasmid

    #209247

    Purpose

    Mammalian expression of ATP10B mutant E210Q

    Depositor

    Hye-Won Shin

    Article

    Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E210Q

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG/ATP8B1(E234Q)-HA

    Plasmid

    #209241

    Purpose

    Mammalian expression of ATP8B1 mutant E234Q

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP8B1 (ATP8B1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E234Q

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG/ATP11B(E180Q)-HA

    Plasmid

    #209250

    Purpose

    Mammalian expression of ATP11B mutant E180Q

    Depositor

    Hye-Won Shin

    Article

    Tone et al FEBS Lett. 2020 Feb;594(3):412-423. doi:

    Insert

    ATP11B (ATP11B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E180Q

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG/ATP9B(E272Q)-HA

    Plasmid

    #209245

    Purpose

    Mammalian expression of ATP9B mutant E272Q

    Depositor

    Hye-Won Shin

    Article

    Tone et al FEBS Lett. 2020 Feb;594(3):412-423. doi:

    Insert

    ATP9B (ATP9B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E272Q

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG/ATP10A(E203Q)-HA

    Plasmid

    #209246

    Purpose

    Mammalian expression of ATP10A mutant E203Q

    Depositor

    Hye-Won Shin

    Article

    Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

    Insert

    ATP10A (ATP10A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E203Q

    Promoter

    Available since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG/ATP8B2(E171Q)-HA

    Plasmid

    #209242

    Purpose

    Mammalian expression of ATP8B2 mutant E171Q

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP8B2 (ATP8B2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E171Q

    Promoter

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP Cav2.2 HA pCAGGS

    Plasmid

    #206054

    Purpose

    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/B-actin

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-FIP200 (1-640)

    Plasmid

    #203542

    Purpose

    Make FIP200 NTD:ATG13:ULK1 complex

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-Myc-KATNB1

    Plasmid

    #160054

    Purpose

    Mammalian expression vector for KATNB1, (Katanin p80) with N-terminal Myc tag. Internal ID: WISP20-16.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNB1 (KATNB1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCA528 AMSHLP 16-218

    Plasmid

    #180609

    Purpose

    Bacterial expression for AMSHLP MIT domain residues 16-218. Internal ID: WISP20-83

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    AMSHLP residues 16-218
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 16-218

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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