159,258 results
-
Plasmid#208141PurposeAiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytesInsertSYFP2
UseAAVTagsExpressionMutationNAPromoterBeta Globin minimal promoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/mStayGold(c4)=UtrCH
Plasmid#212020PurposeFor filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).DepositorInsertUtrCH
UseTagsmStayGold(c4)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE1
Plasmid#90335PurposeNFkB - p50/p65 pathway gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterNFKBAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRE-mClover3-TDP-43ΔNLS
Plasmid#214917Purposeinducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear punctaDepositorInsertTDP-43ΔNLS (TARDBP Human)
UseLentiviralTagsmClover3ExpressionMutationmutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…PromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX313-Firefly luciferase
Plasmid#118017PurposeConstitutive expression of Firefly luciferaseDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2 neo
Plasmid#98292PurposeVariant of lentiCRISPRv2 that confers G418 resistanceDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
LV-AR
Plasmid#213819PurposeLenti-viral expression of human androgen receptor wild-type (WT).DepositorInsertAR (human androgen receptor) (AR Human)
UseLentiviralTagsFlag (N terminal on backbone)ExpressionMammalianMutationNo, it is AR wild-type.PromoterEF-1aAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV rtTA3 Blast (w756-1)
Plasmid#26429Purpose3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, BlastDepositorInsertTetracycline repressor A3 mutant
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
7M8
Plasmid#64839PurposeThis plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.DepositorInsert7M8 cap
UseAAVTagsExpressionMutation7mer insertion in the AAV2 capPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-M
Plasmid#182280PurposeMammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.DepositorInsertcAMPFIRE-M (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRT029
Plasmid#127969Purposedouble-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locusDepositorInsertCLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
v5 eVLP gag-pro-pol
Plasmid#228466PurposeExpresses MMLVgag-pro-pol(Q226P) for producing v5 eVLPsDepositorInsertMMLVgag-pro-pol(Q226P)
UseTagsExpressionMammalianMutationQ226PPromoterCMVAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462) V2.0
Plasmid#62987PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)DepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-OVA
Plasmid#64599PurposeExpression of chicken ovalbumin as model antigen for study of MHC class I and class II presentationDepositorInsertOvalbumin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV_iPE-C_P2A_Csy4
Plasmid#232428PurposeExpression plasmid for iPE-C prime editor RNPs with additional Csy4 protectionDepositorInsertiPE-C–P2A–Csy4
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+HyPer7-NLS
Plasmid#136468PurposeMammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
UseTagsTriple nuclear localization signal of SV40 (simia…ExpressionMammalianMutationPromoterCMV, SP6Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-Rpl22-HA
Plasmid#111811PurposeExpress Rpl22HA in astrocytesDepositorInsertRibosomal protein L22 (Rpl22 Mouse)
UseTagsHAExpressionMutationPromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM4D(Gi)-mCherry
Plasmid#50475PurposeGi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.DepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InserthM4D(Gi)-mCherry
UseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-PEmax-P2A-Puro
Plasmid#228890PurposeLentiviral delivery and expression of PEmax protein with puromycin selection cassette.DepositorInsertsPEmax
Puromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET His6 GST TEV LIC cloning vector (1G)
Plasmid#29655DepositorTypeEmpty backboneUseTags6xHis, GST, and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only