We narrowed to 7,772 results for: aav
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Plasmid#229483PurposeAdenoviral helper plasmid containing E2A, VA RNA I and L4-22k/33k genesDepositorInsertE2A, VA RNA I, L4-22k/33k
UseAAV and AdenoviralExpressionMammalianPromoterCMV (E2A), Ef1a (L4-22k/33k) and wt promoter (VA …Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro Xlone-eGFP
Plasmid#136936Purposedonor plasmid for inducible transgene expression in human cellsDepositorInsertall-in-one tet-on system
UseSynthetic BiologyExpressionMammalianPromoterTRE3GSAvailable SinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro-CAG-GCaMP6s
Plasmid#120896PurposeDonor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.DepositorInsertGCaMP6s
ExpressionMammalianPromoterCAGAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
N-term AAV-ABE8e-SpCas9(S55R) (CA21)
Plasmid#242659PurposeCBh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-SpCas9(S55R) - to be used with eVRQR constructsDepositorInsertpAAV-pCBh-BPNLS(SV40)-TadA8e-gs-SpCas9(D10A;S55R)-[N-term]-NpuN-BPNLS(SV40)-WPRE-bGH_PA
UseAAV and CRISPRTagsBPNLS and NpuN(intein)-BPNLSMutationSpCas9(S55R)PromoterCBhAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMK395 (AAVS1 CMV-OsTIR1(F74A))
Plasmid#159992PurposeExpression of OsTIR1(F74A) in human cellsDepositorInsertCMV-OsTIR1(F74A)
ExpressionMammalianAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - AAVS1 sgRNA
Plasmid#70661PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backboneDepositorInsertsgRNA against AAVS1
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Puro_siKO-2TO
Plasmid#86697PurposeTetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locusDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterH1 2TOAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZFN-AAVS1-KKR
Plasmid#159298PurposeZFN (right) targeting the AAVS1 locus with obligate heterodimer FokIDepositorInsertZFN targeting the AAVS1 locus
ExpressionMammalianPromoterCMV promoterAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro cTnT FUCCI
Plasmid#136935Purposedonor plasmid for cardiac-specific FUCCI expression in human cellsDepositorInsertTroponin T promoter
UseSynthetic BiologyExpressionMammalianPromotercTnTAvailable SinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(AAVS1)-PGKpuroBFP-W
Plasmid#200503PurposeLentiviral vector expressing gRNA targeting human SMARCA4 and AAVS1DepositorAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX552-GFP
Plasmid#107023PurposepX552-CMV-GFP. AAV plasmid for sgRNA cloning, with GFP fusion facilitating FACS sorting of cells. Replaced the hSyn promoter in pX552 from Zhang lab with a CMV promoter.DepositorInsertGFP
UseAAV and CRISPRExpressionMammalianAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS1096 Dual-sgRNA.Design 1
Plasmid#159538PurposeDelivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vectorDepositorInsertNme2Cas9 nuclease with two guide RNA cassettes with promoters
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianPromoterU1aAvailable SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct
Plasmid#176836PurposeTargeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.DepositorInsertdCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
UseUnspecifiedMutationSee Depositor Comments BelowPromoterTet operator (tetracycline inducible expression)Available SinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Pur-CAG-hM4Di-mCherry
Plasmid#80947Purposehuman AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoterDepositorInserthM4Di-mCherry
Tagsmcherry fusionExpressionMammalianPromoterCAG promoterAvailable SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)
Plasmid#85798PurposedonorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression systemDepositorInsertPPP1R12C (PPP1R12C Human)
UseHuman targetingMutationhomology arms for knock-in into first intronAvailable SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 CAG rtTA3 TauWT 2N4R-EGFP
Plasmid#132389PurposeExchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 siteDepositorInserthuman TauWT 2N4R-EGFP (MAPT Synthetic, Human)
UseCRISPR and Cre/LoxTagsEGFPExpressionMammalianPromoterTREtightAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDY1656 STITCHR Gluc AAVS1 payload
Plasmid#234827PurposeGluc exon 2 with 50bp AAVS1 homology armsDepositorInsertGluc exon 2
Available SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Pur-CAG-hM3Dq-mCherry
Plasmid#80948Purposehuman AAVS1 site targeting donor plasmid for knock-in hM3Dq-mCherry expression cassette driven by CAG promoterDepositorInserthM3Dq-mCherry
Tagsmcherry fusionExpressionMammalianPromoterCAG promoterAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry
Plasmid#212829PurposeDoxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.DepositorInsertZIM3-KRAB-dCas9-P2A-mCherry
TagsHA-tag and ZIM3-KRABExpressionBacterial and MammalianPromoterCAG, TetOnAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Neo-TRE-CMV-Cre-rtTA
Plasmid#165457PurposeTetracycline or doxycycline inducible CRE expression from AAVS1 locusDepositorInsertCRE
ExpressionMammalianPromoterTight TRE promoterAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only