We narrowed to 9,638 results for: ada

1. α‐eGFP

   Plasmid

   #160214

   Purpose

   Retroviral construct to express an eGFP-tagged version of the α subunit of AP2

   Depositor

   Sandra Schmid

   Article

   Mino et al Traffic. 2020 Sep;21(9):603-616. doi: 10

   Insert

   α‐eGFP (Ap2a2, Ap2a1 Mouse)
   

   Use

   Retroviral

   Tags

   eGFP at Hinge region

   Expression

   Mutation

   Brain-specific Insert

   Promoter

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. SPACE-NG (pRZ5150)

   Plasmid

   #140244

   Purpose

   CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   SPACE_NG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V82G in TadA*, NG mutations in SpCas9, D10A in Sp…

   Promoter

   CMV

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. miABE

   Plasmid

   #205412

   Purpose

   Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

   Insert

   pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D61A+E85R+H369R+S387R+S457R

   Promoter

   hU6, CAG, CMV

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. DENV4 Rluc Reporter GND mutant Replicon

   Plasmid

   #118586

   Purpose

   For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.

   Depositor

   Radhakrishnan Padmanabhan

   Article

   Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.

   Insert

   DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
   

   Use

   Yeast-e. col shuttle vector prs424

   Tags

   Expression

   Mutation

   The conserved GDD in the NS5 polymerase gene muta…

   Promoter

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CamKII-LGI1-pHluorin

   Plasmid

   #185537

   Purpose

   Expresses LGI1-pHluorin under the CamKII promoter

   Depositor

   Jaime de Juan-Sanz

   Article

   Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

   Insert

   LGI1 (Lgi1 Rat)
   

   Use

   Lentiviral

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SPACE (pRZ1813)

   Plasmid

   #140242

   Purpose

   CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   SPACE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V82G in TadA*, D10A in Cas9, R187W in pmCDA1

   Promoter

   CMV

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOD2048-csnDEG

   Plasmid

   #89368

   Purpose

   MosSCI targeting vector expressing a fusion between a GFP nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression controlled by Posm-6 (ciliated sensory neuron specific)

   Depositor

   Karen Oegema

   Article

   Wang et al Development. 2017 Jun 15. pii: dev.15009

   Insert

   vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
   

   Use

   Targeting vector for mos1 transposon mediated sin…

   Tags

   vhhGFP4 (GFP nanobody)

   Expression

   Mutation

   Promoter

   Posm-6

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

8. SPACE-VRQR (pRZ5137)

   Plasmid

   #140245

   Purpose

   CMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   SPACE_VRQR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V82G in TadA*, VRQR mutations in SpCas9, D10A in …

   Promoter

   CMV

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRY2clust-mCherry-RANK-P2A-CIBNcaax

   Plasmid

   #208613

   Purpose

   Mammalian expression of CRY2clust-mCherry-RANK-P2A-CIBNcaax (Opto-RANKm)

   Depositor

   Takao Nakata, Tomohiro Ishii

   Article

   Takada et al Sci Rep. 2024 Jan 19;14(1):1749. doi: 10

   Insert

   CRY2clust-mCherry-RANK-P2A-CIBNcaax (Cry2 Mustard Weed, Mouse)
   

   Use

   Tags

   CAAX and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNIC-ZB-SPRTNwt-FL

    Plasmid

    #110217

    Purpose

    Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    P296L (see depositor comments below)

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-HA-FbxO11-ΔFbox-pcDNA3.1-

    Plasmid

    #52502

    Purpose

    expresses human FbxO11-ΔFbox in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO11 (FBXO11 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    deletion of Fbox (deleted aa 71-116)

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. SPACE-ΔUGI (pRZ1836)

    Plasmid

    #140243

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V82G in TadA*, D10A in Cas9, R187W in pmCDA1

    Promoter

    CMV

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-HA-FbxO11-Jeff-pcDNA3.1-

    Plasmid

    #52503

    Purpose

    expresses human FbxO11-Jeff mutant in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO11 (FBXO11 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Q491L (Jeff)

    Promoter

    CMV

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAdCMV/V5-DEST-Y705F-STAT3-3xFlag

    Plasmid

    #99261

    Purpose

    Adenoviral vector encoding Flag-tagged murine STAT3 (Y705F)

    Depositor

    Alejandro Adam

    Article

    Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

    Insert

    Signal transducer and activator of transcription 3 - Y705F (Stat3 Mouse)
    

    Use

    Adenoviral

    Tags

    3x-Flag

    Expression

    Mutation

    Y705F (contains a silent mutation: nucleotide 271…

    Promoter

    CMV

    Available since

    Jan. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP Kind2 S159/181/666A

    Plasmid

    #105310

    Purpose

    To study altered downstream functions of phospholylation-site null kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S159/181/666A

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEMHE-MmCENPT-4NS-GFP

    Plasmid

    #237431

    Purpose

    Expresses Mus musculus CENP-T with 4 rat-specific residues in positions that show signs of adaptive evolution; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

    Depositor

    Michael Lampson

    Article

    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

    Insert

    Mus musculus CENP-T with 4 rat-specific residues in HFD (Cenpt Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    432 T to A; 463 I to T, 470 L to E, 485 Q to L

    Promoter

    T7

    Available since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. NST3_pOpOn2.1

    Plasmid

    #228066

    Purpose

    Expresses NST3 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.

    Depositor

    Charles Anderson

    Article

    Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10

    Insert

    NAC SECONDARY WALL THICKENING PROMOTING FACTOR3, NST3 (NAC012 Mustard Weed)
    

    Use

    Dexamethasone inducible vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S for LhGR and pOp6-minimal CaMV35S for NST3

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA5-TEX264-SIM1_mut-cGFP

    Plasmid

    #220212

    Purpose

    Mammalian expression of TEX264 with SIM1 motif mutated, fused at C-terminus to GFP

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    p.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…

    Promoter

    CMV/TetO

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. His-MBP-IST1 N-terminal domain

    Plasmid

    #184805

    Purpose

    Bacterial expression of IST1 N-terminal domain

    Depositor

    James Hurley

    Article

    Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    aa 1-187 only

    Promoter

    T7

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. His-MBP-CHMP1B, S2C

    Plasmid

    #184803

    Purpose

    Bacterial expression of CHMP1B (S2C mutation)

    Depositor

    James Hurley

    Article

    Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

    Insert

    CHMP1B (CHMP1B Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    S2C

    Promoter

    T7

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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