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Lenti_AID-Dam-lamin B1

Plasmid

#119764

Purpose

Lentiviral plasmid for Mammalian Expression

Depositor

Maria-Elena Torres-Padilla

Publication

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

AID-Dam-lamin B1

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Lamp1-mCherry-FRB

Plasmid

#186576

Purpose

Expression of mCherry-FRB at the outer surface of the lysosome using LAMP1

Depositor

Stephen Royle

Publication

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LAMP1 (LAMP1 Human)

Tags

mCherry-FRB

Expression

Mammalian

Promoter

CMV

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBAD_GFP11_T7LysH17A

Plasmid

#59591

Purpose

Expression vector for solubility screening of proteins or protein fragments using a split-GFP assay.

Depositor

Richard Kingston

Publication

Bulloch et al Anal Biochem. 2014 Jul 10. pii: S0003-26

Type

Empty backbone

Tags

6xHis-tag and GFP11

Expression

Bacterial

Promoter

pARA

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-SAC1-FKBP

Plasmid

#108129

Purpose

Recruitable SAC1

Depositor

Gerry Hammond

Publication

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry:SACM1L:FKBP1A(3-108) (SACM1L Human)

Expression

Mammalian

Promoter

CMV

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBluescriptII[attB/Ins1]

Plasmid

#74100

Purpose

"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

Depositor

Ben Szaro

Publication

Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

Inserts

Phi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators

Expression

Bacterial

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1(+) prME (JEV) sdm2

Plasmid

#52881

Purpose

expression of Japanese Encephalitis Virus VLPs in mammalian cells

Depositor

Yorgo Modis

Publication

Nour et al PLoS Pathog. 2013;9(9):e1003585. doi: 10

Insert

prME (POLY Japanese encephalitis virus)

Expression

Mammalian

Mutation

sdm2

Promoter

CMV

Available since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
PCS2+ Cas9

Plasmid

#122948

Purpose

For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Cas9

Use

Synthetic Biology

Promoter

SP6

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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Arl13b-mcherry

Plasmid

#234845

Purpose

A mammalian expression plasmid encoding Arl13b with mcherry at C-terminus.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Arl13b (ARL13B Human)

Expression

Mammalian

Available since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-Aurora A

Plasmid

#198402

Purpose

Expression of EGFP-tagged Aurora A in mammalian cells

Depositor

Kenji Sugimoto

Publication

Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Aurora A

Tags

EGFP

Expression

Mammalian

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EndophilinA1-mRuby2

Plasmid

#171945

Purpose

Expresses Endophilin A1 in mammalian cells

Depositor

Cheng-han Yu

Publication

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Endophilin A1 (SH3GL2 Human)

Tags

mRuby2

Expression

Mammalian

Available since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Flag Intersectin Long (human)

Plasmid

#47393

Depositor

Peter McPherson

Publication

Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

intersectin long (ITSN1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
U6-AsCas12f-sgRNA-v2

Plasmid

#204639

Purpose

Expression of trancated AsCas12f sgRNA in mammalian cells

Depositor

Weixin Tang

Publication

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

trancated AsCas12f sgRNA (sgRNA-v2)

Expression

Mammalian

Promoter

U6

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP×3-N1

Plasmid

#86776

Purpose

A mammalian expression vector with triple EGFP at C-terminus.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTE105

Plasmid

#59400

Purpose

MethyloBrick expression vector, tuf promoter; TcR

Depositor

Tobias Erb

Publication

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptuf

Available since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Rtn3S-GFP

Plasmid

#169035

Purpose

Express human Rtn3S in mammalian cells

Depositor

Gia Voeltz

Publication

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rtn3S (RTN3 Human)

Tags

acGFP1

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGATOR-DTA

Plasmid

#64103

Purpose

Tet inducible, Gateway adapted ROSA26 targeting Vector with DTA

Depositor

Bill Skarnes

Publication

Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rTTA (Gt(ROSA)26Sor Synthetic)

Use

Mouse Targeting; Tet inducible

Expression

Mammalian

Promoter

ROSA26 endogenous

Available since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ACE2(-252)-luc

Plasmid

#31111

Purpose

Luciferase reporter construct for human ACE2 promoter

Depositor

Gerhart Ryffel

Publication

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ACE2 promoter (ACE2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

promoter region -252 to +103

Available since

Oct. 14, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pSF280

Plasmid

#167675

Purpose

Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR-CAG_3C-Twin-Strep

Plasmid

#113901

Purpose

Mammalian protein expression

Depositor

A. Radu Aricescu

Publication

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CAG

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Reb_1 aka PAS619

Plasmid

#71224

Purpose

Expresses wild type R bodies from C. taeniospiralis in pETM11 backbone

Depositor

Pamela Silver

Publication

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebA G53A

Promoter

T7

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only