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HiFi Cas9

Plasmid

#188490

Purpose

Expresses FLAG tagged HiFi Cas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

R691A

Promoter

EF1a

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pBR BsmAIM Kan + p15A BsaIR Amp

Plasmid

#206208

Purpose

Contains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIR

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

BsamAIM and BsaIR

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-EGFP

Plasmid

#30130

Depositor

Marius Wernig

Article

Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

Insert

Enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pinducer 20 DN-KASHΔPPPL

Plasmid

#129280

Purpose

Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

Depositor

Daniel Conway

Article

Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

Insert

SYNE1 (SYNE1 Human)

Use

Lentiviral

Tags

mcherry

Mutation

the last 4 amino acids (PPPL) of the KASH domain …

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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GST-p25

Plasmid

#24896

Depositor

Harish Pant

Article

Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

Insert

p25 (CDK5R1 Human)

Tags

GST

Expression

Bacterial

Mutation

N-terminal truncated version of p35, contains aa …

Available Since

May 17, 2010

Availability

Academic Institutions and Nonprofits only
eSpCas9(1.1)

Plasmid

#138555

Purpose

Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

eSpCas9(1.1)

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti sfGFP-LAMP1-mCherry (pHLARE)

Plasmid

#164478

Purpose

Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.

Depositor

Diane Barber

Article

Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

mCherry and superfolder GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b-hAR-663-919

Plasmid

#89083

Purpose

bacterial expression of human androgen receptor ligand bindng domain amino acid residues 663-919 with histidine tag

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-1

Insert

hAR ligand binding domain (AR Human)

Tags

His

Expression

Bacterial

Mutation

aa 663-919

Promoter

T7

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-Nucleolin

Plasmid

#28176

Depositor

Michael Kastan

Article

Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

Insert

Nucleolin (NCL Human)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP (empty vector)

Plasmid

#172604

Purpose

Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Type

Empty backbone

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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