165,652 results

1. pWH1266-Apra-sgRNA

   Plasmid

   #218372

   Purpose

   sgRNA expression plasmid for CRISPR-intereference

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Insert

   sgRNA expression cassette
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV4-p105

   Plasmid

   #23288

   Depositor

   Warner Greene

   Article

   Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

   Insert

   p105 (NFKB1 Human)
   

   Expression

   Mammalian

   Available Since

   April 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBBR1k-con-GFPuv

   Plasmid

   #106386

   Purpose

   Constitutive trc promoter expressing gfpuv on BBR1 origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX-TRE-dCas9-KRAB-MeCP2-BSD

   Plasmid

   #140690

   Purpose

   Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

   Depositor

   Andrea Califano

   Article

   Tan et al bioRxiv 2021.06.28.449297

   Insert

   Cas9m4-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA364

   Plasmid

   #215956

   Purpose

   Fragmid fragment: (guide cassette) epegRNA expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   dU6:3_v1; BsmBI_v14; BsmBI_v15; evopreQ1_v1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS314-URA3

   Plasmid

   #11004

   Depositor

   Francisco Malagon

   Insert

   URA3 (URA3 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   Wild type

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-28a-mCherry-MED1-IDR

   Plasmid

   #194545

   Purpose

   Bacterial Expression of 6xHis mCherry-MED1-IDR Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   MED1 (MED1 Human)
   

   Tags

   mCherry

   Expression

   Bacterial

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDest-565

   Plasmid

   #11520

   Purpose

   Gateway destination vector for bacterial expression; His,GST tag

   Depositor

   Dominic Esposito

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   6xHis and GST

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b-RvLEAMshort

   Plasmid

   #218122

   Purpose

   Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

   Depositor

   Ci Ji Lim

   Article

   Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

   Insert

   RvLEAMshort
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Truncated form (58-181aa)

   Promoter

   T7

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas9-Geminin

    Plasmid

    #109401

    Purpose

    Expresses Cas9 fusion to Geminin degron domain

    Depositor

    Jean-Paul Concordet

    Article

    Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

    Insert

    Geminin (GMNN Human)
    

    Mutation

    Geminin degron fragment

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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