We narrowed to 1,800 results for: PORA

1. pCMV-OptoFGFR1-Y766F

   Plasmid

   #59777

   Purpose

   Expresses optoFGFR1 with mutation in PLC-gamma binding phosphotyrosine site of FGFR1

   Depositor

   Won Do Heo

   Article

   Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

   Insert

   OptoFGFR1-Y766F (FGFR1 Human, Mustard Weed)
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-dGFP-JARID1C A388P

   Plasmid

   #74778

   Purpose

   retroviral expression of JARID1C A388P

   Depositor

   Giovanni Tonon

   Article

   Rondinelli et al J Clin Invest. 2015 Dec;125(12):4625-37.

   Insert

   JARID1C (KDM5C Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   A388P

   Promoter

   Available since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pCRI011-pYFAC-pyroA-PgpdA-4xcrRNAarrayelcA-TtrpC

   Plasmid

   #140203

   Purpose

   Fungal vector to express an LbCas12a crRNA array targeted to Pelca, to be contransformed with pCRI009 containing PelcA-mCherry in a dLbCas12a-VPR strain as proof-of-concept of CRISPRa.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Insert

   crRNA array elcA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PgpdA

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FB013

   Plasmid

   #119710

   Purpose

   TU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMT

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCherry-3xNLS-OptoJNKi-dsm

   Plasmid

   #89743

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi dark-state mutant
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C450A mutation of AsLov2Jalpha

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCherry-3xNLS-OptoJNKi-lsm

   Plasmid

   #89742

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi lit-state mutant
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   I539E mutation of AsLov2Jalpha

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. FKBP-beta2(616-951)-GFP Double

   Plasmid

   #100729

   Purpose

   To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP2B1 (AP2B1 Human)
   

   Use

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   Long isoform, deletion of clathrin box motif and …

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. FKBP-beta2(616-951)-GFP ∆CBM

   Plasmid

   #100728

   Purpose

   To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP2B1 (AP2B1 Human)
   

   Use

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   Long isoform, deletion of clathrin box motif

   Promoter

   CMV

   Available since

   Sept. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN_TTmiRC2_p53DD

   Plasmid

   #136520

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   p53DD (Trp53 Mouse)
   

   Use

   Entry vector for gateway cloning

   Tags

   3x FLAG

   Expression

   Mutation

   This construct was created by PCR from the pBabe …

   Promoter

   Available since

   Oct. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. FKBP-beta2(616-951)-GFP Y215A

    Plasmid

    #100727

    Purpose

    To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform Y215A mutation (Y815A in full length)

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. SSH-pSUMO-β′-COPI-WD40-Lys17Ala

    Plasmid

    #208171

    Purpose

    Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Lys17Ala mutant (residues 1-301)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    N-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Lys17Ala substitution
    

    Use

    Tags

    His-tag, SUMO-tag, and Strep-tag

    Expression

    Bacterial

    Mutation

    Lys17Ala substitution

    Promoter

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAcBac3-EcLeu TAG-EGFP**

    Plasmid

    #228785

    Purpose

    EcLeu aaRS/tRNA system for TAG suppression with EGFP containing two stop codons for dual suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcLeuRS
    EcLtR
    EGFP**
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Promoter

    Available since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. BD-pGEX5X3-BD-SARS-CoV-2-S-tail-WT

    Plasmid

    #218455

    Purpose

    Bacterial expression plasmid for the SARS-CoV-2 cytosolic tail with an N-terminal GST tag

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    Cytosolic tail of SARS-CoV-2 spike carrying an N-terminal GST-tag (S SARS-CoV-2)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pInducer20 2xHA LTB4R

    Plasmid

    #226810

    Purpose

    Tet-inducible lentiviral vector for 2xHA LTB4R expression

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

    Insert

    LTB4R (LTB4R Human)
    

    Use

    Lentiviral

    Tags

    2xHA tag followed by a GDPPVAT linker

    Expression

    Mutation

    Sequence optimized from NP_858043.1

    Promoter

    Available since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR1A 2xHA GPR153

    Plasmid

    #226808

    Purpose

    Gateway entry vector for 2xHA GPR153

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

    Insert

    GPR153 (GPR153 Human)
    

    Use

    Gateway entry

    Tags

    2xHA tag followed by a GDPPVAT linker

    Expression

    Mutation

    Sequence optimized from NP_997253.2

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR1A 2xHA LTB4R

    Plasmid

    #226806

    Purpose

    Gateway entry vector for 2xHA LTB4R

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

    Insert

    LTB4R (LTB4R Human)
    

    Use

    Gateway entry

    Tags

    2xHA tag followed by a GDPPVAT linker

    Expression

    Mutation

    Sequence optimized from NP_858043.1

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pInducer20 2xHA GPR137

    Plasmid

    #226813

    Purpose

    Tet-inducible lentiviral vector for 2xHA GPR137 expression

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

    Insert

    GPR137 (GPR137 Human)
    

    Use

    Lentiviral

    Tags

    2xHA tag followed by a GDPPVAT linker

    Expression

    Mutation

    Sequence optimized from NP_001164351

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAcBac3-EcTrp-TGA-EGFP**

    Plasmid

    #228786

    Purpose

    EcTrp aaRS/tRNA system for TGA suppression with EGFP containing two stop codons for dual suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTrpRS
    EcWtR
    EGFP**
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TGA suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRXIP-GFP-TRPML1(PHD)

    Plasmid

    #221752

    Purpose

    Stable expression of GFP-TRPML1(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    TRPML1 (MCOLN1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    tandem TRPML1(PHD) (amino acids 1–69)

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. SSH-pcDNA3.1-α-COPI-WD40-Arg13Ala

    Plasmid

    #208172

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 Arg13Ala mutant (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
    

    Use

    Tags

    Strep-tag

    Expression

    Mammalian

    Mutation

    Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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