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pPBO.ACT001

Plasmid

#182711

Purpose

aTc inducible dCasRx

Depositor

Joseph Schoeniger

Article

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

dCasRx

Use

CRISPR

Tags

2x-FLAG

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pTet

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pVS2

Plasmid

#33144

Depositor

Michael Rosbash

Article

Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

Inserts

LacO (256 copies)
GAL-GFP-kd200-GALpA reporter

Expression

Yeast

Mutation

GFP is mutated such that amino acids 64-73 (FSYGV…

Promoter

bidirectional GAL1-GAL10 promoter

Available Since

Dec. 20, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-TRE-DIO-GFP-2A-SynmRuby

Plasmid

#166608

Purpose

Encodes Cre-dependent GFP-2A-synaptophysinmRuby under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

GFP-2A-syn

Use

AAV

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pUB_SM-FLuc0-GFPOPT

Plasmid

#210594

Purpose

Expresses SM(FLAG)-nonoptimal Firefly luciferase-optimal GFP

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal FLuc-Optimal GFP

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_SM-Fluc100-GFPOPT

Plasmid

#210596

Purpose

Expresses SM(FLAG)-optimal Firefly luciferase-optimal GFP

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal FLuc-Optimal GFP

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTH761-CEN-mCherry_v4

Plasmid

#40599

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences

Expression

Yeast

Mutation

mCherry ORF partially codon optimised for yeast e…

Promoter

GPD (TDH3)

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL

Plasmid

#24233

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
Str-KDEL_SBP-mCherry-GPI

Plasmid

#65295

Purpose

synchronize trafficking of mCherry-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pBabepuro-HA-GASC1

Plasmid

#24182

Purpose

Mammalian expression of human JMJD2C with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

GASC1 (KDM4C Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
Str-KDEL_flGalT-SBP-EGFP

Plasmid

#65272

Purpose

synchronize trafficking of GalT from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-Cas9

Plasmid

#166601

Purpose

Encodes Cre-dependent Cas9 under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

Cas9

Use

AAV

Promoter

tetracycline response element

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ING2

Plasmid

#13294

Depositor

Curtis Harris

Article

Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

Insert

ING2 (INGX Human)

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pAceBAC2-MLG-HMF-LST2

Plasmid

#220787

Purpose

Expresses N-terminal His10-Myc-FLAG-LST2 in insect cells

Depositor

Timm Maier

Article

Battaglioni et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

LST2 (ZFYVE28 Human)

Tags

10xHis-Myc-FLAG

Expression

Insect

Promoter

p10

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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myc-mTOR (1-1482 aa)

Plasmid

#21745

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1-1482) (Mtor Rat)

Tags

myc

Expression

Mammalian

Mutation

amino acids 1-1482

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/Zeo-CnB

Plasmid

#191965

Purpose

Expression of CnB (calcineurin subunit beta).

Depositor

Luca Scorrano

Article

Cereghetti et al Proc Natl Acad Sci U S A. 2008 Oct 14;10

Insert

Calcineurin subunit beta (PPP3CB Human)

Expression

Mammalian

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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T7-RelA(KR)

Plasmid

#23251

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA (RELA Human)

Tags

T7 gp10

Expression

Mammalian

Mutation

K218/221R, K310R

Available Since

March 15, 2010

Availability

Academic Institutions and Nonprofits only
mRIP3 GFP wt

Plasmid

#41382

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (Ripk3 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

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HA-MCPIP1

Plasmid

#127459

Purpose

Expressing human MCPIP1 in mammalian cells

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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T7-RelA(K310R)

Plasmid

#23250

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA (RELA Human)

Tags

T7 gp10

Expression

Mammalian

Mutation

K310R

Available Since

March 15, 2010

Availability

Academic Institutions and Nonprofits only
pBSSK WT p85 alpha flag

Plasmid

#13429

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (PIK3R1 Human)

Tags

flag

Expression

Bacterial

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-TVA-oG-mCherry

Plasmid

#166602

Purpose

Encodes Cre-dependent TVA-oG-mCherry under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

TVA-oG-mCherry

Use

AAV

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM60-Ptb

Plasmid

#38245

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Ptb (Ptbp1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pSF4 CMV intron renilla TRICK CTE polyA

Plasmid

#84443

Purpose

TRICK reporter mRNA

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

Renill-TRICK

Use

Luciferase; Frt

Expression

Mammalian

Promoter

Tet-CMV

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM60-Auf1

Plasmid

#38242

Purpose

3rd generation lentiviral vector

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

AUF1 (HNRNPD Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ManII-SBP-EGFP

Plasmid

#65255

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pCEP4_WT_OCT4

Plasmid

#40629

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Expression

Mammalian

Promoter

EF1-alpha

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
Ii-Str_ManII-SBP-mCherry

Plasmid

#65256

Purpose

synchronize trafficking of ManII from the ER

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3 mSmoothened WT

Plasmid

#126408

Purpose

Full length mouse Smoothened cDNA. Expression of Smoothened in mammalian cell lines.

Depositor

Stacey Ogden

Article

Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

Insert

m-Smoothened (Smo Mouse)

Expression

Mammalian

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/TO/V5-DEST-mScarlet

Plasmid

#215536

Purpose

Lentiviral transfer vector to express the mScarlet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

mScarlet

Use

Lentiviral

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2198

Plasmid

#179773

Purpose

Prgef-1 NLF-1::GFP unc-54 3' UTR C.elegans pan-neural expression of NLF-1::GFP

Depositor

Mei Zhen

Article

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1::GFP (nlf-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available Since

March 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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