Skip to main content
Addgene
Showing: 2841 - 2880 of 3521 results

  9. pGB-GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1

    Plasmid
    #187936
    Purpose
    Plasmid for the expression and purification of GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1327
    Depositor
    Sascha Martens
    Article
    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e
    Inserts
    ATG14 (ATG14 Human)
    VPS34 (PIK3C3 Human)
    VPS15 (PIK3R4 Human)
    BECN1 (BECN1 Human)
    Use
    Tags
    GST-mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187936-plasmid-map-sequence-id-374446

    Enlarge

  10. pZP222_INTACT_AT

    Plasmid
    #225992
    Purpose
    To make INTACT lines under desired promoter
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    Tags
    INTACT
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225992-plasmid-map-sequence-id-452361

    Enlarge

  11. pGENT_GW_aco_mScarlet3

    Plasmid
    #225991
    Purpose
    To make gene fusions with Arabidopsis codon-optimized mScarlet3
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    Tags
    mScarlet3
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225991-plasmid-map-sequence-id-448012

    Enlarge

  12. pJC226

    Plasmid
    #198637
    Purpose
    MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system
    Depositor
    Shaun Curtin
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198637-plasmid-map-sequence-id-394770

    Enlarge

  13. x.Amylase

    Plasmid
    #87132
    Purpose
    To make in situ hybridization probe for Xenopus amylase.
    Depositor
    Francesca Spagnoli
    Insert
    Amylase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  14. pJC224

    Plasmid
    #231169
    Purpose
    Empty Module A plasmid compatible with MoClo and Cermak (2017) genome engineering plasmids
    Depositor
    Daniel Voytas
    Type
    Empty backbone
    Use
    Subcloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231169-plasmid-map-sequence-id-460659

    Enlarge

  15. pJC225

    Plasmid
    #231170
    Purpose
    Empty Module B plasmid compatible with MoClo and Cermak (2017) genome engineering plasmids
    Depositor
    Daniel Voytas
    Type
    Empty backbone
    Use
    Subcloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231170-plasmid-map-sequence-id-459161

    Enlarge

  16. pJC227

    Plasmid
    #231172
    Purpose
    Empty Module C' plasmid compatible with MoClo and Cermak (2017) genome engineering plasmids
    Depositor
    Daniel Voytas
    Type
    Empty backbone
    Use
    Subcloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231172-plasmid-map-sequence-id-459157

    Enlarge

  17. pENTR_AtU6p_BsaI_ccdb_gRNA

    Plasmid
    #225986
    Purpose
    To make Gateway entry clone of CRISPR guide RNA under AtU6p
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225986-plasmid-map-sequence-id-448026

    Enlarge

  18. pENTR_AtU6p_BsaI_gRNA

    Plasmid
    #225987
    Purpose
    To make Gateway entry clone of CRISPR guide RNA under AtU6p
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225987-plasmid-map-sequence-id-448010

    Enlarge

  19. pCENTR_DMC1p_amiRNA

    Plasmid
    #225985
    Purpose
    To drive any artificial microRNA under the regulatory control of AtDMC1 promoter and it will make a gateway entry clone
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225985-plasmid-map-sequence-id-448024

    Enlarge

  20. mProx1

    Plasmid
    #87129
    Purpose
    To make in situ hybridization probe for mouse Prox1.
    Depositor
    Francesca Spagnoli
    Insert
    Prox1 (Prox1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    87129-plasmid-map-sequence-id-174062

    Enlarge

  21. RPL3-GFP (96)

    Plasmid
    #24040
    Depositor
    Michael Rout
    Insert
    RPL3 (RPL3 Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 31, 2010
    Availability
    Academic Institutions and Nonprofits only

  22. Hsp68-CreER(TM) (DM#264)

    Plasmid
    #15028
    Depositor
    Douglas Melton
    Insert
    Cre-ER(TM)
    Use
    Cre/Lox; For making transgenic constructs
    Tags
    Hsp68 promoter and polyA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 18, 2007
    Availability
    Academic Institutions and Nonprofits only
    15028-plasmid-map-sequence-id-152167

    Enlarge

  23. 2PSW

    Plasmid
    #51342
    Depositor
    Cheryl Arrowsmith
    Insert
    2PSW
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. pBS AID-3xF_PP

    Plasmid
    #235502
    Purpose
    Vector to make C-Term AID fusions
    Depositor
    Brian Hendrich
    Insert
    Auxin Inducible Degron
    Use
    Mouse Targeting
    Tags
    AID, 3xFLAG
    Expression
    Mutation
    None
    Promoter
    PGK
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    235502-plasmid-map-sequence-id-471037

    Enlarge

  25. pMK33-GW-TurboID-V5

    Plasmid
    #196281
    Purpose
    Gateway destination vector to express C-terminal TurboID fusion under metallothionein promoter, for making stable cell lines
    Depositor
    Norbert Perrimon
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    196281-plasmid-map-sequence-id-384554

    Enlarge

  26. pMK33-V5-TurboID-GW

    Plasmid
    #196282
    Purpose
    Gateway destination vector to express N-terminal TurboID fusion under metallothionein promoter, for making stable cell lines
    Depositor
    Norbert Perrimon
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    196282-plasmid-map-sequence-id-384562

    Enlarge

  27. mOrange in pBSDONR P4r-P2

    Plasmid
    #121529
    Purpose
    For making C-terminal mOrange fusions using multisite Gateway cloning
    Depositor
    Roger Innes
    Insert
    mOrange
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    121529-plasmid-map-sequence-id-234042

    Enlarge

  28. pZP06B

    Plasmid
    #232321
    Purpose
    Make sacB a counterselection marker to replace galK
    Depositor
    Chenggang Wu
    Insert
    PrpsJ-sacB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232321-plasmid-map-sequence-id-462085

    Enlarge

  29. pBCG10

    Plasmid
    #232323
    Purpose
    Make mazF a counterselection marker to replace sacB in pZP06C
    Depositor
    Chenggang Wu
    Insert
    mazF-tetR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232323-plasmid-map-sequence-id-462282

    Enlarge

  30. pWALIUM10-V5-TurboID-GW

    Plasmid
    #196285
    Purpose
    Gateway destination vector to express N-terminal TurboID fusion under UAS promoter, for making transgenic flies
    Depositor
    Norbert Perrimon
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    196285-plasmid-map-sequence-id-384638

    Enlarge

  31. pWALIUM10-GW-TurboID-V5

    Plasmid
    #196283
    Purpose
    Gateway destination vector to express C-terminal TurboID fusion under UAS promoter, for making transgenic flies
    Depositor
    Norbert Perrimon
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    196283-plasmid-map-sequence-id-385988

    Enlarge

  32. sCFP in pBSDONR P4r-P2

    Plasmid
    #121530
    Purpose
    For making C-terminal sCFP fusions using multisite Gateway cloning
    Depositor
    Roger Innes
    Insert
    sCFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    121530-plasmid-map-sequence-id-234035

    Enlarge

  33. pRetroQ-Cre-ERT2

    Plasmid
    #59701
    Purpose
    to make Cre-ERT2 expressing stable cell line by retrovirus
    Depositor
    Richard Youle
    Insert
    Cre-ERT2
    Use
    Retroviral
    Tags
    Estrogen receptor (ERT)
    Expression
    Mammalian
    Mutation
    Promoter
    cmvIE
    Available since
    Sept. 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    59701-plasmid-map-sequence-id-93474

    Enlarge

  34. mouse ptc probe M2-3

    Plasmid
    #58701
    Purpose
    makes mouse ptc probe for in situ hybridization
    Depositor
    Matthew Scott
    Insert
    ptc (Ptch1 Mouse)
    Use
    In situ probe
    Tags
    Expression
    Bacterial
    Mutation
    only EcoRI fragment (5' end)
    Promoter
    T7
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. Sperm whale Mb 64Y68F in pVP80K

    Plasmid
    #86956
    Purpose
    Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).
    Depositor
    John Olson
    Insert
    Mb 64Y68F
    Use
    Tags
    Expression
    Bacterial
    Mutation
    64Y68F
    Promoter
    T5
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    86956-plasmid-map-sequence-id-176792

    Enlarge

  38. pHAGE-CMV-Rheb(D60K)-IRES-eGFP-W

    Plasmid
    #32522
    Depositor
    Mustafa Sahin
    Insert
    dominant negative Rheb (D60K) (Rheb Rat)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D60K to make dominant negative
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  39. pHAGE-CMV-Rheb(D60I)-IRES-eGFP-W

    Plasmid
    #32521
    Depositor
    Mustafa Sahin
    Insert
    Dominant negative Rheb (D60I) (Rheb Rat)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D60I to make dominant negative
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  40. pBrg1-GGSG_AID_3xF_PP Target2

    Plasmid
    #235523
    Purpose
    Targeting construct to make C-term fusion with GGSG-AID-3xFLAG, roxed PgkPuro
    Depositor
    Brian Hendrich
    Insert
    Smarca4 (Smarca4 Mouse)
    Use
    Mouse Targeting
    Tags
    AID, 3xFLAG
    Expression
    Mutation
    None
    Promoter
    PGK
    Available since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    235523-plasmid-map-sequence-id-474040

    Enlarge

Showing: 2841 - 2880 of 3521 results