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We narrowed to 62,913 results for: cloning

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Showing: 1421 - 1440 of 62913 results

  1. pSBtet-BP

    Plasmid
    #60496
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and puromycin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    16 Articles
    60496-plasmid-map-sequence-id-97551

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  3. pL97

    Plasmid
    #78576
    Purpose
    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoter
    Depositor
    Xuming Mao
    Publication
    Sun et al Annals of Microbiology Dec 2012, Volume
    Type
    Empty backbone
    Use
    Streptomyces expression
    Expression
    Bacterial
    Promoter
    ermEp* promoter from S. erythraea
    Available since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    78576-plasmid-map-sequence-id-421926

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  7. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid
    #71684
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    71684-plasmid-map-sequence-id-129672

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  9. pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66

    Plasmid
    #168787
    Purpose
    Donor vector for recombinase mediated integration in filamentous fungi. Is a shuttle vector for optional in vivo cloning in yeast, and contains four inducible promoters or sites for whole BGC cloning.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102
    Type
    Empty backbone
    Use
    Cre/Lox and Synthetic Biology; Expression in fila…
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    168787-plasmid-map-sequence-id-353783

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  13. pET MBP mClover LIC cloning vector (MBP-mClover)

    Plasmid
    #86930
    Purpose
    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mClover
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86930-plasmid-map-sequence-id-174752

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  17. pCS2+N-3xFlag Cloning Vector

    Plasmid
    #196651
    Purpose
    N-terminal 3xFlag mammalian expression plasmid
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    196651-plasmid-map-sequence-id-387125

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  18. pC5Kan-P2A

    Plasmid
    #51814
    Purpose
    P2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single message
    Depositor
    Barry Ganetzky
    Publication
    Daniels et al PLoS One. 2014 Jun 19;9(6):e100637. doi:
    Insert
    P2A peptide (PTV1gp1 porcine teschovirus-1, Synthetic)
    Use
    Shuttle vector for insect expression vectors
    Mutation
    codons altered for Drosophila and to eliminate re…
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    51814-plasmid-map-sequence-id-79554

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  20. pSBbi-RH

    Plasmid
    #60516
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    60516-plasmid-map-sequence-id-97530

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Showing: 1421 - 1440 of 62913 results