We narrowed to 3,800 results for: golden gate

1. pK3-g3g4-K4

   Plasmid

   #218218

   Purpose

   Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   AtU6 promoters and sgRNA scaffolds
   

   Use

   CRISPR

   Available Since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pK5-g5g6-K6

   Plasmid

   #218219

   Purpose

   Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   AtU6 promoters and sgRNA scaffolds
   

   Use

   CRISPR

   Available Since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAIP4_6IYE

   Plasmid

   #234237

   Purpose

   Heterologous protein expression of peptidyl-tRNA hydrolase (PTH) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   6IYE (pth Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. MUT_S1-H1

   Plasmid

   #218394

   Purpose

   3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Expression

   Mammalian

   Promoter

   tetracycline-responsive promoter

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAIP4_2WWE

   Plasmid

   #234210

   Purpose

   Heterologous protein expression of PI3K-C2-gamma in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   2WWE (PIK3C2G Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)

   Plasmid

   #219687

   Purpose

   Level 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

   Depositor

   Jennifer Nemhauser

   Insert

   T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
   

   Expression

   Plant

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPtGG-1

   Plasmid

   #89990

   Purpose

   pBR322-based entry vector for Gateway cloning

   Depositor

   Philip Weyman

   Article

   Bielinski et al PeerJ Preprints. 2017 5:e2907v1

   Type

   Empty backbone

   Use

   Golden gate entry vector

   Available Since

   June 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMV_AA100

   Plasmid

   #245322

   Purpose

   Cas9 CRISPRko all-in-one positive control; targets CD55

   Depositor

   John Doench, David Root

   Insert

   Cas9, sgRNA CD55 (CD55 Synthetic)
   

   Use

   Lentiviral

   Mutation

   None

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMV_AA104

   Plasmid

   #245326

   Purpose

   Cas9 CRISPRa positive control guide; targets CD4

   Depositor

   John Doench, David Root

   Insert

   sgRNA CD4 (CD4 Synthetic)
   

   Use

   Lentiviral

   Mutation

   None

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTwist-Kan-HC-L0-TaBSS1-3D

    Plasmid

    #242008

    Purpose

    L0 3U* Golden Gate part of 3' UTR and downstream regulatory regions of Triticum aestivum MANY-NODED DWARF 1 (MND1) B-subgenome homoeolog (TraesCS7B02G413900)

    Depositor

    Cristobal Uauy

    Insert

    TaBSS1-3D
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAttPF3Hyg

    Plasmid

    #244929

    Purpose

    attP Hyg Resistant plasmid for RMI targeting an attB site

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Phic31 recombinase mediated insertion (rmi)

    Expression

    Worm

    Promoter

    none

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAttBFGNeo2

    Plasmid

    #244928

    Purpose

    attB Neo Resistant mNeonGreen marked plasmid for RMI targeting an attP site

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Phic31 recombinase mediated insertion vector

    Expression

    Worm

    Promoter

    none

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAttBFGNeo

    Plasmid

    #244925

    Purpose

    attB Neo Resistant mNeonGreen marked plasmid for RMI targeting an attP site

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Phic31 recombinase mediated insertion (rmi)

    Expression

    Worm

    Promoter

    none

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAttBFNeo

    Plasmid

    #244923

    Purpose

    attB Neo Resistant plasmid for RMI targeting an attP site

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Phic31 recombinase mediated insertion (rmi)

    Expression

    Worm

    Promoter

    none

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAttBFNeo2

    Plasmid

    #244924

    Purpose

    attB Neo Resistant plasmid for RMI targeting an attP site

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Phic31 recombinase mediated insertion (rmi)

    Expression

    Worm

    Promoter

    none

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAttPF3Neo

    Plasmid

    #244930

    Purpose

    attP Neo Resistant plasmid for RMI targeting an attB site

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Phic31 recombinase mediated insertion (rmi)

    Expression

    Worm

    Promoter

    none

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLII-proUBI10_CKA3 KD K63M,N170D-FLAG_termNOS _OLE-RED

    Plasmid

    #238523

    Purpose

    Plant expression of flag-tagged A. thaliana CK2alpha3 KD K63M,N170D; with OLE1-RED marker

    Depositor

    Michael Hothorn

    Insert

    AtCK2alpha3 (AT2G23080 Mustard Weed)
    

    Tags

    FLAG

    Expression

    Plant

    Mutation

    K63M N170D

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSG1016-2xBsaI-SaKKH_P2A_EGFP

    Plasmid

    #239461

    Purpose

    Codon-optimized S. aureus KKH Cas9. 2x BsaI sites for easy cloning of varying deaminases. EGFP can serve as a transfection marker.

    Depositor

    Alexis Komor

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. MERS-CoV Mpro strain HCoV-EMC 3CL protease domain

    Plasmid

    #228646

    Purpose

    Bacterial expression of the MERS-CoV Mpro domain for biochemical assay.

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    MERS-Cov Mpro domain
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Available Since

    March 28, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pAIP4_1T3Y

    Plasmid

    #234228

    Purpose

    Heterologous protein expression of coactosin-like protein in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1T3Y (COTL1 Human)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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