We narrowed to 1,621 results for: nif

1. pBLO 62.5

   Plasmid

   #123124

   Purpose

   Human expression vector for planctomyces CasX and sgRNA

   Depositor

   Benjamin Oakes, Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Inserts

   CasX protein
   CasX sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pATZ-HA-GFP11

   Plasmid

   #177720

   Purpose

   ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFP

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   alpha-1-antitrypsin (SERPINA1 Human)
   

   Use

   Tags

   GFP11 and HA

   Expression

   Bacterial and Mammalian

   Mutation

   E342K (ATZ mutant)

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4.23-Fosprom

   Plasmid

   #188113

   Purpose

   Expresses luciferase under control of Fos promoter in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Shchuka et al PLoS One. 2023 Jan 3;18(1):e0271081. doi

   Insert

   Fos promoter (Fos Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Fos endogenous promoter

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJJGL002

   Plasmid

   #180606

   Purpose

   Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Insert

   PlmCasX
   

   Use

   CRISPR

   Tags

   10xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. CoV2-N-P.1

   Plasmid

   #177959

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P80R,R203K,G204R

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pyc111f

   Plasmid

   #158780

   Purpose

   ribosome-tagged, Cre-dependent GCaMP for soma localization

   Depositor

   Zachary Knight, Jennifer Garrison

   Article

   Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

   Insert

   GCaMP6f-RPL10a
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9

   Plasmid

   #186699

   Purpose

   Plasmid encoding PRDM9dC-Cas9 fusion under CMV promoter

   Depositor

   Jennifer Doudna

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

   Insert

   PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9 (PRDM9 Human)
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pyc111m

   Plasmid

   #158779

   Purpose

   ribosome-tagged, Cre-dependent GCaMP for soma localization

   Depositor

   Zachary Knight, Jennifer Garrison

   Article

   Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

   Insert

   GCaMP6m-RPL10a
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lentiMutB3GALT5catdead-blast

   Plasmid

   #220869

   Purpose

   lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   encodes D156A and D243A mutations; also, nucleoti…

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CoV2-N-R203K

    Plasmid

    #177951

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203K). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (R203K) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R203K

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mApple-M1 SpastinE442Q

    Plasmid

    #134464

    Purpose

    M1 SpastinE442Q expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Glu to Gln at amino acid 442

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Cerulean-GalT

    Plasmid

    #11930

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Cole et al Science. 1996 Aug 9. 273(5276):797-801.

    Insert

    GalT (GALT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amino acids 1-60 of the human galactosyltransfera…

    Promoter

    Available since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Empty Target Control

    Plasmid

    #127667

    Purpose

    Encodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoter

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBLO ddd64.1

    Plasmid

    #123121

    Purpose

    E. coli expression vector for catalytically inactivated deltaproteobacteria CasX

    Depositor

    Benjamin Oakes, Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    CasX
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Mutated amino acids in catalytic triad to alanine…

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mApple-M1 SpastinK388R

    Plasmid

    #134463

    Purpose

    M1 SpastinK388R expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lys to Arg at amino acid 388

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CoV2-N-G204R

    Plasmid

    #177953

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (G204R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (G204R) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G204R

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CMV-hCas9-T2A-mStrawberry

    Plasmid

    #187224

    Purpose

    Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Inserted T2A-mStrawberry at 3' terminal of M…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SERPINA-bio-His

    Plasmid

    #52182

    Purpose

    Expresses full-length Alpha-1-antitrypsin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SERPINA (SERPINA1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mApple-M11-92

    Plasmid

    #134466

    Purpose

    M11-92 expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin Amino Acids 1-92 (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M1 Spastin Amino Acids 1-92

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PBLO CasX-gRNA

    Plasmid

    #126419

    Purpose

    E. coli vector for CasX gRNA --- Used for In Vitro Transcription

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    casX gRNA
    

    Use

    CRISPR; In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge