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iLID-GFP-GCN4-ppKin14

Plasmid

#240420

Purpose

-end oriented kinesin for optogenetic pulling experiments

Depositor

Anna Akhmanova

Article

Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

Insert

ppKin14Vib

Tags

GCN4, GFP, and iLID

Expression

Mammalian

Mutation

deleted amino acids 1-850

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mBaoJin-P2A-mCherry

Plasmid

#229601

Purpose

Expresses mBaoJin and mCherry in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mBaoJin

Use

AAV

Expression

Mammalian

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTXB1-EXO1b D173A

Plasmid

#68269

Purpose

Bacterial expression of human EXO1 D173A (catalytically-dead) mutant

Depositor

Stefano Ferrari

Article

Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10

Insert

EXO1 (EXO1 Human)

Tags

Mxe intein/chitin binding domain

Expression

Bacterial

Mutation

catalytically-dead D173A. Please see depositor co…

Promoter

T7

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pMSCVpuro-eGFP-mcGAS

Plasmid

#108675

Purpose

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged mouse cGAS

Depositor

Andrew Jackson, Martin Reijns

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

eGFP-cGAS (Cgas Mouse)

Use

Retroviral

Promoter

LTR

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETB/mStayGold(c4)

Plasmid

#212018

Purpose

For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

mStayGold(c4)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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AY27_pU6.gRNA.CLYBL

Plasmid

#199238

Purpose

Expression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locus

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

Insert

S. pyogenes gRNA spacer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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CMV_3xFLAG_NLS_ISCro4_NLS_SV40_polyA

Plasmid

#251493

Purpose

Expression of a human codon optimised ISCro4 bridge recombinase

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 bridge recombinase

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.774_775insHV

Plasmid

#82852

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

HV insertion between AA 774 and 775

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRRL-MHCK7-GCaMP6

Plasmid

#65042

Purpose

Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

Depositor

Nenad Bursac

Article

Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

Insert

GCaMP6

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

MHCK7

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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prsTagRFP-N1

Plasmid

#31925

Depositor

Vladislav Verkhusha

Article

Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Insert

rsTagRFP

Expression

Mammalian

Mutation

F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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