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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,358 results for: BLI;

Showing: 2881 - 2920 of 8358 results

  1. GSK-3beta S9A (Xenopus)/pCS2+

    Plasmid
    #16333
    Depositor
    Peter Klein
    Insert
    GSK-3beta (gsk3b.L Frog)
    Use
    Vertebrate expression
    Tags
    Expression
    Mutation
    GSK-3 with serine-9 to alanine mutation
    Promoter
    Available since
    Dec. 21, 2007
    Availability
    Academic Institutions and Nonprofits only

  2. pHAGE-hSPC-TFEBeGFP-W

    Plasmid
    #126692
    Purpose
    Lineage specific over expression of TFEB in lung alveolar epithelial cells
    Depositor
    Darrell Kotton
    Insert
    TFEB (TFEB Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deleted Stop Codon on TFEB
    Promoter
    hSPC
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    126692-plasmid-map-sequence-id-245688

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  3. pCMV-PE2-pegRNA-ST2-com-vector

    Plasmid
    #136272
    Purpose
    Expresses PE2 and ST2-com modified pegRNA cassette
    Depositor
    Baisong Lu
    Insert
    PE2, pegRNA scaffold
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    136272-plasmid-map-sequence-id-273277

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  4. pHH0103 NEDD4L WW domain #4

    Plasmid
    #104207
    Purpose
    Bacterial expression of WW domain #4 from NEDD4L
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    NEDD4L WW domain #4 (NEDD4L Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104207-plasmid-map-sequence-id-202579

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  5. pLK89 - Edit-directing plasmid cloning insert

    Plasmid
    #98846
    Purpose
    Contains the insert used for step 2 of edit-directed plasmid cloning
    Depositor
    Leonid Kruglyak
    Insert
    KanR
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Changed sequence to remove a MluI cut site
    Promoter
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    98846-plasmid-map-sequence-id-189230

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  6. pGEX6P1-Reps1-EH domain

    Plasmid
    #36860
    Depositor
    Yvonne Groemping
    Insert
    Reps1 (REPS1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-225 and 315-769
    Promoter
    Available since
    July 17, 2012
    Availability
    Academic Institutions and Nonprofits only
    36860-plasmid-map-sequence-id-46057

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  7. ScSpe1_pET20b(+)

    Plasmid
    #118343
    Purpose
    Expresses the His tagged Ornithine Decarboxylase from Saccharomyces cerevisiae in Escherichia coli BL21 or similar strains
    Depositor
    Markus Ralser
    Insert
    SPE1
    Use
    Tags
    Histidine
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    118343-plasmid-map-sequence-id-229413

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  8. pJK367

    Plasmid
    #72406
    Purpose
    Produces Acetobacter aceti 1023 Est06
    Depositor
    T. J. Kappock
    Insert
    lipase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    lacks Met1 and Thr2 in annotated (v1) sequence
    Promoter
    T7
    Available since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72406-plasmid-map-sequence-id-134674

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  9. pHH0103 C20orf67 WW domain

    Plasmid
    #104273
    Purpose
    Bacterial expression of WW domain from C20orf67
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    C20orf67 WW domain (PCIF1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104273-plasmid-map-sequence-id-203480

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  10. pLV Ndc1-V5

    Plasmid
    #175115
    Purpose
    Lentiviral expression of mouse Ndc1-V5
    Depositor
    Larry Gerace
    Insert
    Ndc1 (Ndc1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175115-plasmid-map-sequence-id-351773

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  11. LCB7_crtYB

    Plasmid
    #140232
    Purpose
    Expression of gene coding lycopene bifunctional β-cyclase for production beta-carotene from lycopene
    Depositor
    Kang Zhou
    Insert
    phytoene-beta carotene synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    K307E
    Promoter
    pthrC3
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    140232-plasmid-map-sequence-id-288146

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  12. pAAV-CAG-FLEX-splitTVA-EGFP

    Plasmid
    #59332
    Purpose
    Expresses splitTVA-P2A-EGFP
    Depositor
    Ian Wickersham
    Insert
    splitTVA950-P2A-EGFP
    Use
    AAV and Cre/Lox
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    59332-plasmid-map-sequence-id-92028

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  13. pFastBacI/MDR3cysless

    Plasmid
    #74400
    Purpose
    expression of MDR3 in insect cells
    Depositor
    Robert Nakamoto
    Insert
    MDR3 (Abcb1a Mouse)
    Use
    Tags
    10His
    Expression
    Insect
    Mutation
    all Cys to Ala
    Promoter
    Polyhedrin
    Available since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only

  14. pHH0103 WWP2 WW domain #1

    Plasmid
    #104212
    Purpose
    Bacterial expression of WW domain #1 from WWP2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WWP2 WW domain #1 (WWP2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104212-plasmid-map-sequence-id-204807

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  15. pHH0103 WWP2 WW domain #3

    Plasmid
    #104214
    Purpose
    Bacterial expression of WW domain #3 from WWP2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WWP2 WW domain #3 (WWP2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104214-plasmid-map-sequence-id-204291

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  16. pHH0103 WWP2 WW domain #2

    Plasmid
    #104213
    Purpose
    Bacterial expression of WW domain #2 from WWP2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WWP2 WW domain #2 (WWP2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104213-plasmid-map-sequence-id-204203

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  17. pHH0103 WWP2 WW domain #4

    Plasmid
    #104215
    Purpose
    Bacterial expression of WW domain #4 from WWP2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WWP2 WW domain #4 (WWP2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104215-plasmid-map-sequence-id-204195

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  18. pSB700-ACTC1-Puro

    Plasmid
    #128324
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against human ACTC1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128324-plasmid-map-sequence-id-254244

    Enlarge

  19. p(+)MV-NSE-FlagrP-3g_haP-F497A-3p

    Plasmid
    #58799
    Purpose
    Expresses recombinant measles virus with duplicated P gene. wt P gene and P-F497A gene are tagged with Flag and HA peptides and si1 (siGFP) and si2 (siP) target sequences, respectively.
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Use
    Tags
    Flag and Ha
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    58799-plasmid-map-sequence-id-91094

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  20. pAG472 H2B-iFAST

    Plasmid
    #130818
    Purpose
    Expresses H2B-iFAST in mammalian cells (nuclear labeling)
    Depositor
    Arnaud Gautier
    Insert
    H2B-iFAST
    Use
    Tags
    myc-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    130818-plasmid-map-sequence-id-254956

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  21. pExp-IF2

    Plasmid
    #112567
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and IF2 (domain I of translation initiation factor IF-2 from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-IF2 and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112567-plasmid-map-sequence-id-217215

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  22. pJK402

    Plasmid
    #72415
    Purpose
    Produces Acetobacter aceti 1023 Est03, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    4-hydroxybenzoyl-CoA thioesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72415-plasmid-map-sequence-id-135527

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  23. pJK580

    Plasmid
    #71694
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin with a C-terminal His6 tag (AaTrxAH6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71694-plasmid-map-sequence-id-134489

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  24. pLIX403-EYFP-ccdB-EBFP2

    Plasmid
    #158553
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158553-plasmid-map-sequence-id-309848

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  25. pTXB3-Flag PLK1 T210D

    Plasmid
    #68272
    Purpose
    bacterial expression of Plk1 T210D mutant
    Depositor
    Stefano Ferrari
    Insert
    PLK1 (PLK1 Human)
    Use
    Tags
    Flag and Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    T210D
    Promoter
    T7
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  26. pCAGGS-S-P1-FLAG

    Plasmid
    #177095
    Purpose
    P1 variant S-protein with c-terminal FLAG tag
    Depositor
    Daniel Conway
    Insert
    S-protein P1 (S SARS-CoV-2)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177095-plasmid-map-sequence-id-345528

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  27. Paxillin_PSCFP2_ mEOS2

    Plasmid
    #114192
    Purpose
    studying focal adhesion proteins by superresolution microscopy (PALM) with dual labelling
    Depositor
    Aleksandra Radenovic
    Insert
    Paxillin (PXN Chicken)
    Use
    Tags
    PS-CFP2 and mEos2
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    114192-plasmid-map-sequence-id-225613

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  28. pLV V5-Sun1

    Plasmid
    #175113
    Purpose
    Lentiviral expression of V5-tagged mouse Sun1
    Depositor
    Larry Gerace
    Insert
    Sun1 (Sun1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175113-plasmid-map-sequence-id-351771

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  29. pBS3ndd

    Plasmid
    #97354
    Purpose
    Low background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, kanamycin resistant
    Depositor
    Jamorn Somana
    Insert
    Nucleoid Disruptive Domain
    Use
    Cloning
    Tags
    Expression
    Bacterial
    Mutation
    changed methionine 1 to isoleucine
    Promoter
    E. coli lacZ promoter
    Available since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    97354-plasmid-map-sequence-id-191848

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  30. Clim-DC1-mCherry-H2B-Venus

    Plasmid
    #129559
    Purpose
    nuclear marker
    Depositor
    Inaki Ruiz-Trillo
    Insert
    mcherry and Histone 2B fused to venus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    129559-plasmid-map-sequence-id-258976

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  31. 4ROJ

    Plasmid
    #64661
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    VAV2:JMC092-C08:C231327 (VAV2 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    May 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  32. HC080

    Plasmid
    #59545
    Purpose
    Expresses yeast Atg13 under the endogenous promoter with a GFP N-terminal tag
    Depositor
    Daniel Klionsky
    Insert
    ATG13 (ATG13 Budding Yeast)
    Use
    Tags
    GFP
    Expression
    Bacterial and Yeast
    Mutation
    AvrII site created after start codon
    Promoter
    ATG13
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  33. pORTMAGE302A

    Plasmid
    #128970
    Purpose
    Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with high temperature induction
    Depositor
    George Church
    Inserts
    PapRecT
    mutL E32K
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E32K
    Promoter
    cI-pL
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    128970-plasmid-map-sequence-id-252505

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  34. PB CAG-R-3xSV40pA-R-td-sfGFP

    Plasmid
    #177150
    Purpose
    CAG tdGFP dre/rox reporter piggyBac transposon (3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Dre / rox ; piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177150-plasmid-map-sequence-id-348857

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  35. Abr in pAcG2T

    Plasmid
    #38162
    Depositor
    Nora Heisterkamp
    Insert
    ABR (ABR Human)
    Use
    Tags
    GST
    Expression
    Insect
    Mutation
    Promoter
    polyhedron
    Available since
    Aug. 27, 2012
    Availability
    Academic Institutions and Nonprofits only

  36. U6-Lin28aIR

    Plasmid
    #120530
    Purpose
    dsRNA expression
    Depositor
    Petr Svoboda
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120530-plasmid-map-sequence-id-233972

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  37. pRK GFP Vinculin

    Plasmid
    #50531
    Depositor
    Kenneth Yamada
    Insert
    Vinculin (VCL Chicken)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  38. pAAV-synP-FLEX-EGFP-B19G

    Plasmid
    #59333
    Purpose
    Expresses EGFP-P2A-B19G
    Depositor
    Ian Wickersham
    Insert
    EGFP-P2A-B19G
    Use
    AAV and Cre/Lox
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin-1
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    59333-plasmid-map-sequence-id-92029

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  39. pAc5.1B-EGFP-DmRBM7

    Plasmid
    #66594
    Purpose
    expresses EGFP-tagged RBM7 in Drosophila cells
    Depositor
    Elisa Izaurralde
    Insert
    RBM7 (CG11454 Fly)
    Use
    Drosophila expression
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Actin 5C
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    66594-plasmid-map-sequence-id-113870

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  40. pTWEEN-miR-224

    Plasmid
    #35683
    Depositor
    George Calin
    Insert
    nsa-mir-224 (MIR224 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 2, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 2881 - 2920 of 8358 results