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Showing: 3601 - 3650 of 9181 results

  3. pET21-Flavidin

    Plasmid
    #89881
    Purpose
    Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling
    Depositor
    Mark Howarth
    Article
    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-
    Insert
    Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
    Use
    Tags
    6 glutamate tag (C terminal on insert)
    Expression
    Bacterial
    Mutation
    K80R, K121R, K132R, K134R
    Promoter
    Available since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89881-plasmid-map-sequence-id-177281

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  10. pET28a-HsSENP1

    Plasmid
    #71465
    Purpose
    Encodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 1 (SENP1 Human)
    Use
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pSNK

    Plasmid
    #135193
    Purpose
    Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassette
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    I-SceI lamba Red
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    araBAD
    Available since
    April 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    135193-plasmid-map-sequence-id-288121

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  16. pCNA

    Plasmid
    #135191
    Purpose
    Arabinose inducible expression of lamba-Red recombinase proteins and I-CreI.
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    I-CreI lamba Red
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    araBAD
    Available since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135191-plasmid-map-sequence-id-273788

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  19. pDR111_Bgal NLS

    Plasmid
    #188396
    Purpose
    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Insert
    lacZ
    Use
    Tags
    PhoD secretion peptide and SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Phyper spank
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    188396-plasmid-map-sequence-id-373478

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  22. pDEINO4

    Plasmid
    #179488
    Purpose
    Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation
    Depositor
    Bogumil Karas
    Article
    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1
    Insert
    aadA1
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    179488-plasmid-map-sequence-id-360447

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  27. pRXG

    Plasmid
    #113643
    Purpose
    This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    mRFP1-linker-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    113643-plasmid-map-sequence-id-217116

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  30. pCB11

    Plasmid
    #201111
    Purpose
    pSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.
    Depositor
    Mary Dunlop
    Article
    Lugagne et al Nat Commun. 2024 Mar 8;15(1):2148. doi:
    Inserts
    CcaR
    tetA(C)
    sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C-to-T transition at pos. no. 1049 of tetA(C) lea…
    Promoter
    J23100 and PcpcG2-172
    Available since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    201111-plasmid-map-sequence-id-403029

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  41. pSNA

    Plasmid
    #135192
    Purpose
    Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassette
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    I-SceI lamba Red
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    araBAD
    Available since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135192-plasmid-map-sequence-id-296242

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  47. pTU2S-a (colE1 origin)

    Plasmid
    #74091
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    None
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74091-plasmid-map-sequence-id-138318

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  48. pRJPaph-lacZYA

    Plasmid
    #67037
    Purpose
    Plasmid for Paph-lacZYA integration downstream of B. diazoefficiens (B. japonicum) scoI
    Depositor
    Julia Vorholt
    Article
    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.
    Inserts
    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    lacZYA
    Use
    Conjugative bacterial vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67037-plasmid-map-sequence-id-115553

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Showing: 3601 - 3650 of 9181 results