We narrowed to 5,097 results for: puc
-
Plasmid#162998Purposeplasmid expressing ABE with dCas9DepositorInsertdABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianPromoterEF1-aAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT15002-ABEmax
Plasmid#163006Purposeplasmid expressing ABEmaxDepositorInsertABEmax
UseCRISPRTagsSV40 NLSExpressionMammalianPromoterEF1-aAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT15064-eCBE
Plasmid#163009Purposeplasmid expressing eCBEDepositorInserteCBE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianPromoterEF1-aAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)WF
Plasmid#108337PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAID5VY
Plasmid#165048PurposeIntegrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…Available SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFPQ3
Plasmid#83558PurposeUsed to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.DepositorInsertGAL2 promoter-yEGFP
ExpressionYeastPromoterGAL2Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
S8_attL1-N-term-EGFP-attL2
Plasmid#128472PurposeEGFP entry clonesDepositorInsertEGFP entry clones
ExpressionBacterialAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW063
Plasmid#115950PurposeDestination VectorDepositorInsertLvl0 SGP (+/- Apt) Dest
UseSynthetic BiologyAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGGC001
Plasmid#48824PurposeProvides WUS as GreenGate coding sequence module.DepositorInsertWUS (WUS Mustard Weed)
UseGolden gate compatible cloning vectorMutationinternal BsaI recognition site removed by silent …Available SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
AP1297‐1
Plasmid#87247Purposegtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)DepositorInsertgtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
Tagsgtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…Available SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJBL712
Plasmid#140382PurposeExpresses OtrR-regulated 3WJdB RNA aptamer driven by T7 promoter with the otrO sequence 5BP downstream from the promoterDepositorInsertT7-otrO-3WJdB-T
UseSynthetic BiologyAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH71
Plasmid#128214Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAP1680
Plasmid#137088PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with tagRFPDepositorInserttagRFP
UsePcr template for donor dnaExpressionBacterialAvailable SinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)BR
Plasmid#108336PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)WF
Plasmid#108333PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)BF
Plasmid#108334PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)WR
Plasmid#108335PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BF
Plasmid#108338PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)WR
Plasmid#108339PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BR
Plasmid#108340PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW322
Plasmid#115953PurposeDestination VectorDepositorInsertLvl1 P1 With 3'UTR
UseSynthetic BiologyAvailable SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW323
Plasmid#115954PurposeDestination VectorDepositorInsertLvl1 P1 No 3'UTR
UseSynthetic BiologyAvailable SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW325
Plasmid#115956PurposeDestination VectorDepositorInsertLvl1 P2 With 3'UTR
UseSynthetic BiologyAvailable SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW326
Plasmid#115957PurposeDestination VectorDepositorInsertLvl1 P2 No 3'UTR
UseSynthetic BiologyAvailable SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW327
Plasmid#115958PurposeDestination VectorDepositorInsertLvl1 P2 End
UseSynthetic BiologyAvailable SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW328
Plasmid#115959PurposeDestination VectorDepositorInsertLvl1 P3 With E1-3'UTR
UseSynthetic BiologyAvailable SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pATM (pKD-G11)
Plasmid#62690PurposeMammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancerDepositorInsertamiR-eGFP123/amiR-eGFP419/tdTomato
UseRNAiExpressionMammalianAvailable SinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pADH118-17
Plasmid#91727PurposeNAT-marked C. albicans URA3-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.DepositorInsertNAT 2of2, pSNR52, C. albicans URA3-specific gRNA, LEU2 2of2
Available SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADH143-2
Plasmid#90990PurposeNAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. maltosa LEU2 2 of 2
UseCRISPRAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADH100-2
Plasmid#90981PurposeNAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, FRT, DS_HIS1
UseCRISPRAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGS1044
Plasmid#63797PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal 3xFLAG tag and a LEU2 selection markerDepositorInsert3xFLAG followed by KlLEU2
UseE. coli cloning vectorTags3xFLAGPromoternoneAvailable SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKH032
Plasmid#224548Purpose2A-3xNLS-2xClover mouse Car2 C-term targeting vectorDepositorInsert2xClover
UseMouse TargetingAvailable SinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKH040
Plasmid#224549Purpose2A-3xNLS-tdTomato mouse Car2 C-term targeting vectorDepositorInserttdTomato
UseCRISPR and Mouse TargetingAvailable SinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIVT-mRuby2-P2A-PuroR_pKG02755
Plasmid#246371PurposeIVT template for making modRNA encoding mRuby2-P2A-PuroRDepositorInsertmRuby2-P2A-PuroR
UseSynthetic Biology; In vitro transcriptionExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
CIRC-V1-Tet-CVB3 IRES-EGFP-BbsI
Plasmid#246756PurposeRNA circularization with CIRC using Tet intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI.DepositorInsertCIRC-V1-Tet-CVB3 IRES-EGFP-BbsI
UseSynthetic BiologyAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
AGG1767 PUb-Bs-TRE-Bn
Plasmid#242441PurposeAe aegypti PUb promoter expressing barstar, and TRE expressing barnaseDepositorInsertsBarstar
DsRED
barnase
ExpressionInsectPromoterAePUb, Hr5/IE1, and TREAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
apoBb.1-Dendra2
Plasmid#218180Purposeused for TALEN HDR to generate endogenous fusion of HA-Dendra2 to apoBb.1 in zebrafishDepositorInsertapoBb.1-Dendra2
UseTALENTagsfuses HA-Dendra2 to apoBb.1Mutationinserts HA-Dendra2 sequence at C-terminus of apoB…Available SinceAug. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS1376
Plasmid#239829PurposeWT OrufIscB omegaRNA mammalian expressionDepositorInsertOrufIscB omegaRNA scaffold
ExpressionMammalianPromoterhuman U6Available SinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILFPZ1A9
Plasmid#223835PurposeExpressing a cytosolic pH sensor (mTagBFP2-mNeonGreen) in Saccharomyces cerevisiaeDepositorInsertpTEF1>mTagBFP>mNeonGreen>
ExpressionYeastAvailable SinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILFPQ13A7
Plasmid#223837PurposeExpressing mScarletI3 under the control of the TEF1 promoter in Saccharomyces cerevisiaeDepositorInsertpTEF1>mScarletI3>
ExpressionYeastPromoterTEF1Available SinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILFPQ13B7
Plasmid#223838PurposeExpressing StayGold under the control of the TEF1 promtoer in Saccharomyces cerevisiaeDepositorInsertpTEF1>StayGold>
ExpressionYeastPromoterTEF1Available SinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILFPmTagBFP2FrFAST
Plasmid#223839PurposeExpressing mTagBFP2-FrFAST under the control of the TEF1 promtoer in Saccharomyces cerevisiaeDepositorInsertpTEF1>mTagBFP2>FrFAST
ExpressionYeastPromoterTEF1Available SinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDCX_mEmerald_CRISPR_HDR
Plasmid#239331PurposeHDR template to insert mEmerald before the stop codon in exon 7 of the human doublecortin (DCX) locusDepositorInsertmEmerald and DCX homology arms
UseCRISPRPromoterNoneAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYI277
Plasmid#228285PurposeConstitutive EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoterKpGAPDH promoterAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMDW108
Plasmid#227194PurposeTemplate for multi-nucleosome arrayDepositorInsert64mer array of p601 with 50bp flanking naked DNA between the anchor points and the first and the last 601.
ExpressionBacterialPromoterPlacAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
AA498
Plasmid#231119PurposeFragmid fragment: (guide cassette) ABE activity-based selection CD274 positive controlDepositorInsertsgCD274 + ABE splice-targeting sgRNA
UseCRISPRExpressionBacterialAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
AA505
Plasmid#231120PurposeFragmid fragment: (guide cassette) CBE activity-based selection CD274 positive controlDepositorInsertsgCD274 + CBE splice-targeting sgRNA
UseCRISPRExpressionBacterialAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHBJT002
Plasmid#225153PurposeHigh copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGA-pTRE-B
Plasmid#218894Purposegolden gate cloningDepositorInsertTRE
UseSynthetic BiologyMutation580G>CAvailable SinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGF-bGH polyA-G
Plasmid#218902Purposegolden gate cloningDepositorInsertbGH polyA
UseSynthetic BiologyAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only