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BirA-Myc_N_ETS1

Plasmid

#167700

Purpose

Expresses ETS1 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ETS1 (ETS1 Human)

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-CUL1 K720A

Plasmid

#19939

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 K720A (CUL1 Human)

Tags

HA2

Expression

Mammalian

Mutation

CUL1 with a NEDD8 modification mutant

Available Since

April 17, 2009

Availability

Academic Institutions and Nonprofits only
hHSF1 K80Q

Plasmid

#118351

Purpose

Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.

Depositor

Lea Sistonen

Article

Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K80Q

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ14806

Plasmid

#239273

Purpose

Expresses TRF1-PYL1 for recruiting RNA to the nuclear stress body via CRISPR-TO

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

HSF1-BFP-PYL1 (HSF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-DEST47-SIRT1

Plasmid

#242131

Purpose

Expression in mammalian cells

Depositor

J. Carl Barrett, Izumi Horikawa

Article

Michishita et al Mol Biol Cell. 2005 Oct;16(10):4623-35.

Insert

SIRT1 (SIRT1 Human)

Tags

Cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-CUL1 Y42A/M43A

Plasmid

#19940

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 Y42A/M43A (CUL1 Human)

Tags

HA2

Expression

Mammalian

Mutation

CUL1 with a SKP1 binding mutant

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pLenti Dest PuroR R1-R2

Plasmid

#84575

Purpose

Gateway destination vector expressing Puromycin resistance under a PGK promoter

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-ARP8

Plasmid

#15361

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

actin-related protein 8 (ACTR8 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-CUL1 delta 610-615

Plasmid

#19941

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 delta 610-615 (CUL1 Human)

Tags

HA

Expression

Mammalian

Mutation

CUL1 with a ROC1 binding mutant

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pBabe(puro) mouse Egr3

Plasmid

#108102

Purpose

Retroviral vector for expression of mouse Egr3

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr3 (Egr3 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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p21-pRc/CMV

Plasmid

#20814

Depositor

William Kaelin

Article

Adams et al Mol Cell Biol. 1996 Dec . 16(12):6623-33

Insert

p21 (CDKN1A Human)

Expression

Mammalian

Mutation

wt

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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HOXB4 (human) pSG5

Plasmid

#8527

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Use

In vitro transcriptioin/translation

Expression

Mammalian

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-CUL1 delta C22

Plasmid

#19942

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 delta C22 (CUL1 Human)

Tags

HA2

Expression

Mammalian

Mutation

Mutation of 22 amino acids from the C terminus

Available Since

April 17, 2009

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf170

Plasmid

#12858

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf170

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-FNIP1

Plasmid

#49175

Purpose

Mammalian expression of mouse folliculin interacting protein 1 (FNIP1) fused to GFP

Depositor

Shawn Ferguson

Article

Petit et al J Cell Biol. 2013 Sep 30;202(7):1107-112

Insert

Folliculin Interacting Protein 1 (Fnip1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Stop codon deleted

Promoter

CMV

Available Since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf106

Plasmid

#12794

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf106

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
Cathepsin S S21C/C25A

Plasmid

#84695

Purpose

for expression of mutant Cathepsin S in Leishmania tarantolae

Depositor

Hans Brandstetter

Article

Freier et al Sci Rep. 2015 Aug 3;5:12707. doi: 10.103

Insert

cathepsin S (CTSS Human)

Use

Expression in leishmania tarantolae

Tags

his-tag

Mutation

S21C, C25A

Promoter

unknown

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR1A DHFR.cHSF1

Plasmid

#134737

Purpose

Gateway entry vector for constitutively active HSF1 fused to the DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

Insert

DHFR.cHSF1 (HSF1 Human)

Use

Gateway Cloning

Tags

DHFR

Mutation

Deletion of amino acids 186-202

Promoter

None

Available Since

Oct. 23, 2021

Availability

Academic Institutions and Nonprofits only

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SaLgCP

Plasmid

#164562

Purpose

Puro

Depositor

Junwei Shi

Article

Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Flag-NLS-SaCas9-P2A-PuroR

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf45

Plasmid

#12733

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf45

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only