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pEF1a-RoninDHSA-FLAG-IRES-Neo

Plasmid

#28021

Depositor

Thomas Zwaka

Article

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Insert

Ronin (THAP11 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

DHSA mutation (Y246A) of the previously defined H…

Promoter

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pQC hKGA.wt V5-His IRES G418

Plasmid

#110390

Purpose

γ-Retroviral transfer vector for expressing glutminase isoform KGA (wild type), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.

Depositor

Sandra Martha Gomes Dias

Insert

KGA glutaminase (GLS Human)

Use

Retroviral

Tags

6xHis and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Ki67 (shuffled Nterm)-mNeonGreen-IRESpuro2

Plasmid

#183744

Purpose

Expresses Ki67 (shuffled Nterm)-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 (shuffled Nterm) (MKI67 Human)

Use

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

CD domain with shuffled 4 fragments fused to the …

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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b2AR->M71-IRES-tauGFP-ACNF TV

Plasmid

#105198

Purpose

targeting vector to generate a replacement of CDS of M71 with CDS of mouse beta2-adrenergic receptor with expression of tauGFP.

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

b2AR->M71-IRES-tauGFP-ACNF (Adrb2, Olfr151 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Flag-Avi-ires-CHD5

Plasmid

#68878

Purpose

Transient mammalian expression of CHD5 for BirA tagging

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Tags

FLAG/AVI

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pLEX-MAPKAP1Del-FHH-IRES-Puro

Plasmid

#120706

Purpose

Expresses MAPKAP1 Truncation-Flag-HA-6xHIS fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

MAPKAP1 Isoform 1

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Deleted amino acids 193-522

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NLS-IRES-hrGFP

Plasmid

#107543

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NLS (APP Human)

Use

AAV

Tags

Expression

Mutation

None

Promoter

CMV

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCru5-/UAA-mEGFP-IRES-mCherry

Plasmid

#49225

Purpose

Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

Depositor

Clifford Wang

Article

Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

Inserts

mEGFP
mCherry

Use

Retroviral and Synthetic Biology

Tags

SfuI site to create C-terminal fusions

Expression

Mammalian

Mutation

Promoter

Viral LTR (or CMV)

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-bat-Caspase-4

Plasmid

#183377

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-4

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

MESV

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1a-myc-LRH1(ES)-IRES-neo

Plasmid

#28092

Depositor

Austin Cooney

Article

Wagner et al Stem Cells. 2010 Oct . 28(10):1794-804.

Insert

Lrh-1 (Nr5a2 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
pLV-EF1a-KIF5A-HA-IRES-Puro

Plasmid

#166952

Purpose

Lentiviral plasmid expressing HA-tagged KIF5A protein with IRES-Puro from the EF1a promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

eF1a

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-EVA1ext-hFcIgG3-IRES-YFP

Plasmid

#64926

Purpose

To express extracellular portion of EVA1 (mouse) fused with human Fc(IgG3)

Depositor

Pau Serra

Article

Garabatos et al Monoclon Antib Immunodiagn Immunother. 2

Insert

mouse EVA1 fused with human FcIgG3 (Mpzl2, IGHG3 Mouse, Human)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only
pFUGW-shRIIα-RIIαWT-IRES-EGFP

Plasmid

#183456

Purpose

Replacement of endogenous rat PKA-RIIα subunits with wild-type RIIα

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Tags

Expression

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEJS507-pCDest2-noAcr-mTagBFP2-IRES

Plasmid

#85748

Purpose

Expresses mTagBFP2 only; used as a control

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

mTagBFP2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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KA2897_pPB-TREtight-UBCmin-cHA-IRESH2BmKO2

Plasmid

#124176

Purpose

piggyBac-based Dox-inducible expression (moderate)

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B IRES GFP

Plasmid

#133817

Purpose

Encodes the C2B domain of synaptotagmin 1 for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Expression

Mutation

Promoter

hSyn (human synapsin I promoter)

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcna1a-IRES-EGFP

Plasmid

#164965

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcna1a (kcna1a Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Expression

Mutation

Promoter

actinb (actb2) promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-pax3a-kcnj13-IRES-EGFP

Plasmid

#164950

Purpose

Tol2 construct: pax3a-5.4k promoter driven zebrafish kcnj13 fused with EGFP and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Expression

Mutation

Promoter

pax3a promoter -5.4k

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj13-IRES-EGFP

Plasmid

#164941

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Expression

Mutation

Promoter

actinb (actb2) promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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Drd1a->M71-IRES-tauGFP ACNF TV

Plasmid

#105073

Purpose

Targeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-447 of Drd1a and an IRES-tauGFP followed by ACNF cassette

Depositor

Peter Mombaerts

Article

Katidou et al Mol Cell Neurosci. 2018 Apr;88:138-147.

Insert

Drd1a->M71-IRES-tauGFP-ACNF (Drd1, Olfr151 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Drd1a coding sequence followed by IRES-tauGFP ACNF

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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