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We narrowed to 2,449 results for: PORA

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Showing: 1441 - 1460 of 2449 results

  1. pCRI011-pYFAC-pyroA-PgpdA-4xcrRNAarrayelcA-TtrpC

    Plasmid
    #140203
    Purpose
    Fungal vector to express an LbCas12a crRNA array targeted to Pelca, to be contransformed with pCRI009 containing PelcA-mCherry in a dLbCas12a-VPR strain as proof-of-concept of CRISPRa.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Insert
    crRNA array elcA
    Use
    CRISPR and Synthetic Biology
    Promoter
    PgpdA
    Available Since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    140203-plasmid-map-sequence-id-310119

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  2. FB013

    Plasmid
    #119710
    Purpose
    TU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available Since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119710-plasmid-map-sequence-id-237145

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  3. pmCherry-3xNLS-OptoJNKi-dsm

    Plasmid
    #89743
    Purpose
    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    NLS-OptoJNKi dark-state mutant
    Use
    Fluorescent protein
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    C450A mutation of AsLov2Jalpha
    Promoter
    CMV
    Available Since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89743-plasmid-map-sequence-id-192503

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  4. pmCherry-3xNLS-OptoJNKi-lsm

    Plasmid
    #89742
    Purpose
    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    NLS-OptoJNKi lit-state mutant
    Use
    Fluorescent protein
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    I539E mutation of AsLov2Jalpha
    Promoter
    CMV
    Available Since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89742-plasmid-map-sequence-id-192502

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  9. SSH-pSUMO-β′-COPI-WD40-Lys17Ala

    Plasmid
    #208171
    Purpose
    Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Lys17Ala mutant (residues 1-301)
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    N-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Lys17Ala substitution
    Tags
    His-tag, SUMO-tag, and Strep-tag
    Expression
    Bacterial
    Mutation
    Lys17Ala substitution
    Available Since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    208171-plasmid-map-sequence-id-472121

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  15. SSH-pcDNA3.1-α-COPI-WD40-Arg13Ala

    Plasmid
    #208172
    Purpose
    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 Arg13Ala mutant (residues 1-327)
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
    Tags
    Strep-tag
    Expression
    Mammalian
    Mutation
    Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    208172-plasmid-map-sequence-id-432648

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  16. SSH-pcDNA3.1-α-COPI-WD40-Arg300Ala

    Plasmid
    #208173
    Purpose
    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg300Ala (residues 1-327)
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys,Arg300Ala)
    Tags
    Strep-tag
    Expression
    Mammalian
    Mutation
    Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe19…
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    208173-plasmid-map-sequence-id-436198

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  17. SSH-pcDNA3.1-α-COPI-WD40-Lys15Ala

    Plasmid
    #208174
    Purpose
    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Lys15Ala (residues 1-327)
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
    Tags
    Strep-tag
    Expression
    Mammalian
    Mutation
    Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    208174-plasmid-map-sequence-id-436200

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  19. SSH-pcDNA3.1-SARS-CoV-2-S-Lys1269Ala-His1271Ala

    Plasmid
    #218463
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Lys1269Ala and His1271Ala substitutions)
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    SARS-CoV-2 spike protein (1273 residues) carrying Lys1269Ala and His1271Ala substitutions (S )
    Expression
    Mammalian
    Mutation
    Lys1269Ala and His1271Ala substitutions
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218463-plasmid-map-sequence-id-436199

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Showing: 1441 - 1460 of 2449 results