We narrowed to 9,096 results for: sgRNA

Showing: 1441 - 1460 of 9096 results

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pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102797

Purpose

Retrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Publication

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

H231L

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pML104-KanMX-sgRNAv2

Plasmid

#254712

Purpose

Yeast CRISPR-Cas9 backbone with sgRNA cassette for rapid PCR-based guide insertion; KanMX/G418 selection in S. cerevisiae, Amp/Kan propagation in E. coli.

Depositor

Brian Strahl

Publication

Rostamian et al STAR Protoc. 2026 Jul 28;7(3):104751. do

Insert

YKU70 sgRNA (YKU70 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52 promoter

Available since

Aug. 18, 2026

Availability

Academic Institutions and Nonprofits only

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(pRZ294) AAV: U6-gRNA-EF1a-ZsGreen

Plasmid

#254586

Purpose

AAV backbone expressing a U6-driven sgRNA with SapI spacer and ZsGreen from the EF1a promoter

Depositor

Jesse Engreitz

Insert

sgRNA with SapI spacer

Use

AAV

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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(pRZ115) AAV: U6-gRNA-EF1a-mCherry

Plasmid

#254584

Purpose

AAV backbone expressing a U6-driven sgRNA with SapI spacer and mCherry from the EF1a promoter

Depositor

Jesse Engreitz

Insert

sgRNA with SapI spacer

Use

AAV

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pSEM255 - sgRNA mosTI Pmlc-2_GFP

Plasmid

#159835

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI Pmlc-2_GFP

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE

Plasmid

#85453

Purpose

Lentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.

Depositor

Aviv Regev

Publication

Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

Insert

U6-sgRNA-Backbone-UP-TdTomato

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

deletion of AA123-365 in tdTomato (please see dep…

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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SL2-sgSat-MTSb/BFP/pdCas9-C1

Plasmid

#162761

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting centromeres

Depositor

Antony K Chen

Publication

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9 and sgRNA(SL2-sgSat-MTSb)

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pYC1446

Plasmid

#158720

Purpose

Integrating plasmid expressing Sth1 Cas9 and the cognate sgRNA

Depositor

Yi-Cheng Sun

Publication

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

Sth1 sgRNA scaffold

Use

CRISPR

Expression

Bacterial

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPT24-mtArg8m*-V5-UTR-HH-sgRNA_ATP6-HDV

Plasmid

#251981

Purpose

For biolistic transformation to express Arg8m* and control sgRNA targeting ATP6 from mtDNA

Depositor

Alice Ting

Publication

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Inserts

mtArg8m*
HH-sgRNA_ATP6-HDV

Tags

V5

Expression

Yeast

Mutation

premature stop CAA->TAA, Q79*

Available since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pPT24-mtArg8m*-V5-UTR-HH-ntsgRNA-HDV

Plasmid

#251982

Purpose

For biolistic transformation to express Arg8m* and a non-targeting control sgRNA from mtDNA

Depositor

Alice Ting

Publication

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Inserts

mtArgm*
HH-nt sgRNA-HDV

Tags

V5

Expression

Yeast

Mutation

premature stop CAA->TAA, Q79*

Available since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA-RMP64 (pAVA3500)

Plasmid

#239237

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP64

Depositor

Andrei Alexandrov

Publication

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP64

Use

CRISPR and Lentiviral

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA-RMP24 (pAVA3547)

Plasmid

#239240

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

Depositor

Andrei Alexandrov

Publication

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP24

Expression

Mammalian

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA-RMP24 (pAVA3555)

Plasmid

#239242

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

Depositor

Andrei Alexandrov

Publication

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP24

Use

CRISPR and Lentiviral

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA-RMP24 (pAVA3563)

Plasmid

#239258

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

Depositor

Andrei Alexandrov

Publication

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP24

Use

CRISPR and Lentiviral

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA-RMP64 (pAVA3572)

Plasmid

#239259

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP64

Depositor

Andrei Alexandrov

Publication

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP64

Use

CRISPR and Lentiviral

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pJJF143_SECGFP_sg1

Plasmid

#163864

Purpose

sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Publication

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting GFP

Use

CRISPR

Expression

Worm

Promoter

R07E5.16 (U6)

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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p236_LTJ_sgRNAFoxp3sf/J

Plasmid

#82676

Purpose

sgRNA targeting murine Foxp3 scurfy. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Publication

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting Foxp3 sf/J

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Cdk12_intron3_sg2_pX330

Plasmid

#127174

Purpose

sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

Depositor

Phil Sharp

Publication

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 3 sgRNA

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Availability

Academic Institutions and Nonprofits only

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Cdk12_intron4_sg1_pX458

Plasmid

#127175

Purpose

sgRNA that cuts within intron 4 of mouse CDK12 genomic locus in pX458 backbone

Depositor

Phil Sharp

Publication

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 4 sgRNA

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Availability

Academic Institutions and Nonprofits only

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pJK398

Plasmid

#155370

Purpose

dCas9 sgRNA oscillator v1 (dCRv1) (p15A)

Depositor

Pamela Silver

Publication

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Insert

dCas9 sgRNA oscillator v1

Use

CRISPR

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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