165,688 results

1. pcDNA-dCas9

   Plasmid

   #47106

   Purpose

   Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Flag and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1-H-Luc_cyto

   Plasmid

   #113929

   Purpose

   Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   H-Luc
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-UCOE-SFFV-EGFP

   Plasmid

   #188900

   Purpose

   Human lentiviral vector for expression of EGFP from a SFFV promoter with an upstream ubiquitous chromatin opening element

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   EGFP
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCM184

   Plasmid

   #46012

   Depositor

   Mary Lidstrom

   Article

   Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

   Type

   Empty backbone

   Use

   Cre/Lox

   Promoter

   na

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD-HisD-miniGFP1

   Plasmid

   #188896

   Purpose

   Express 6xHis-Tag miniGFP1 in E.coli

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   miniGFP1
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB_TRE3G_scFV-sfGFP-Dot1LCD

   Plasmid

   #235582

   Purpose

   Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dot1l fused with scFV to programme H3K79me2

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   Dot1l (Dot1l Mouse)
   

   Use

   CRISPR

   Available Since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. CRISPR-StAR4BN (Lenti)

   Plasmid

   #222692

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PGK without BsmBI cutsite

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. B52 (empty plasmid backbone to express 2 sgRNAs)

   Plasmid

   #100708

   Purpose

   Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoters

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pP3-GFP

   Plasmid

   #53436

   Purpose

   Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   P3 promoter
   

   Use

   Synthetic Biology

   Tags

   none

   Expression

   Bacterial

   Mutation

   none

   Promoter

   P3

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-C3-Sec15

    Plasmid

    #53760

    Purpose

    pEGFP-C3 plasmid that expresses Sec15

    Depositor

    Channing Der

    Article

    Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

    Insert

    Sec15 (EXOC6 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only