165,652 results

1. qTAG-AAVS1-PGK-Zeo

   Plasmid

   #227271

   Purpose

   Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-ING2(pHD)

   Plasmid

   #21589

   Depositor

   Junying Yuan

   Article

   Gozani et al Cell. 2003 Jul 11. 114(1):99-111.

   Insert

   GFP-ING2(pHD) (Ing2 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains Ing2 plant homeodomain (PHD) amino acids…

   Available Since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pFRT-TODestFLAGHA_HuB

   Plasmid

   #65755

   Purpose

   expresses FLAGHA-tagged HuB in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL2 (ELAVL2 Human)
   

   Tags

   FLAG HA and V5

   Expression

   Mammalian

   Promoter

   CMV, 2x Tet

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTD-NTwinStrep_Km

   Plasmid

   #45938

   Purpose

   use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Tags

   6xHis and Twin-Strep

   Expression

   Bacterial

   Promoter

   Trc (lactose/IPTG inducible)

   Available Since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC18T-mini-Tn7T-Tp-dsRedExpress

   Plasmid

   #65036

   Purpose

   TpR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   dsRedExpress
   

   Expression

   Bacterial

   Available Since

   Aug. 14, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCFJ2059 - [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)

   Plasmid

   #159872

   Purpose

   Three-fragment Gateway compatible protein tag for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-GRAM1b

   Plasmid

   #211494

   Purpose

   Expression of EGFP-tagged GRAM domain of GRAMD1b (EGFP-GRAM1b) in mammalian cells

   Depositor

   Yasunori Saheki

   Article

   Naito et al Elife. 2019 Nov 14;8. pii: 51401. doi: 1

   Insert

   GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV4-Tax WT

   Plasmid

   #23284

   Depositor

   Warner Greene

   Article

   Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

   Insert

   Tax
   

   Expression

   Mammalian

   Available Since

   April 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGPN-TMPRSS2

   Plasmid

   #187484

   Purpose

   Expression vector - TMPRSS2

   Depositor

   Lewis Cantley

   Article

   Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Use

   Lentiviral

   Promoter

   EFS

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 mycBioID

    Plasmid

    #35700

    Purpose

    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    cmv

    Available Since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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