163,353 results

1. enIscB

   Plasmid

   #205410

   Purpose

   Vector encoding human codon-optimized enhanced-activity OgeuIscB driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

   Insert

   pCAG_Flag_SV40NLS_OgeuIscB*_npNLS_polyA_pU6__RNA*_pCMV_mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E85R+H369R+S387R+S457R

   Promoter

   CAG, hU6, CMV

   Available since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB025

   Plasmid

   #183092

   Purpose

   Expresses FnCas12a in Bacteroides and used for genome editing

   Depositor

   Lei Dai

   Article

   Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

   Insert

   FnCas12a, gRNA, Promoter, HAB, TetR,
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P1TDPGH023

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDualIN-SMN2

   Plasmid

   #214430

   Purpose

   Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

   Insert

   SMN2 EXON 7 (SMN2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-puro GFP-p62

   Plasmid

   #38277

   Depositor

   Noboru Mizushima

   Article

   Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

   Insert

   Sequestosome-1 (Sqstm1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. R619-M66-303: CMV51p> HCoV-OC43 S-2P-T4f-3C-His8-Strep2x2

   Plasmid

   #166015

   Purpose

   mammalian expression of HCoV-OC43 soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   HCoV-OC43 S (spike)
   

   Use

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSEVA3b67Rb

   Plasmid

   #58311

   Purpose

   Shuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.

   Depositor

   Tom Ellis

   Article

   Wright et al ACS Synth Biol. 2014 May 13.

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCK302

   Plasmid

   #87768

   Purpose

   As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used

   Depositor

   John Heap

   Article

   Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

   Inserts

   PrhaBAD-sfGFP
   rhaS from E. coli with its native RBS from E. coli
   

   Use

   Synthetic Biology

   Tags

   6xHis Tag

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaBAD rhamnose-inducible promoter from E. coli …

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3 Flag MKK7B2Jnk1a1(APF)

   Plasmid

   #19730

   Depositor

   Roger Davis

   Article

   Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

   Insert

   MKK7B2Jnk1a1(APF) (MAPK8, Map2k7 Mouse, Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   replaced dual phosporylation motif Thr(1959)-Pro-…

   Promoter

   Available since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1α-F-FLEX-mNaChBacMut-T2A-tdTomato

   Plasmid

   #60660

   Purpose

   Flp-dependent expression of mNaChBacMut-T2A-tdTomato

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   FLEX-mNaChBacMut-T2A-tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   E191K

   Promoter

   EF1α

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUW-tetO-Pu.1

    Plasmid

    #139839

    Purpose

    doxycycline-inducible expression of mouse Pu.1 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Pu.1 (Spi1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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