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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 3,176 results for: UBC

Showing: 2191 - 2220 of 3176 results

  1. iCytSnFR

    Plasmid
    #177738
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iCytSnFR
    Use
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    177738-plasmid-map-sequence-id-348679

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  2. iCyt_BrEt_SnFR

    Plasmid
    #177740
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iCyt_BrEt_SnFR
    Use
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    177740-plasmid-map-sequence-id-349387

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  3. iCyt_F_SnFR

    Plasmid
    #177739
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iCyt_F_SnFR
    Use
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    177739-plasmid-map-sequence-id-348682

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  5. pMinDis.X513-iNicSnFR3b-(V7)-ER

    Plasmid
    #125123
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine
    Depositor
    Henry Lester
    Article
    Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018
    Insert
    iNicSnFR3a-(V7)-ER
    Use
    Tags
    Fusion protein -Endoplasmic retention motif
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Available since
    Oct. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    125123-plasmid-map-sequence-id-245436

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  8. pCIG1_UL136_26kDa_Myc

    Plasmid
    #74955
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 26kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    Strep II Tag (GGGGWSHPQFEKGGGGWSHPQFEK)
    Expression
    Mammalian
    Mutation
    delta (1-62) M78A M80A M100A M128A
    Promoter
    CMV, Lentiviral LTR
    Available since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74955-plasmid-map-sequence-id-141778

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  9. pCIG1_UL136_33kda_Myc

    Plasmid
    #74957
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 33kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    Myc tag (EQKLISEEDL)
    Expression
    Mammalian
    Mutation
    M63A M78A M80A M100A M128A
    Promoter
    CMV, Lentiviral LTR
    Available since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74957-plasmid-map-sequence-id-141779

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  26. LV-indLS2

    Plasmid
    #123068
    Purpose
    Lentiviral construct that delivers a tamoxifen inducible Cre. Upon recombination, luc2 and mStrawberry express. Used to image spontaneous tumorigenesis or subclonal cell populations in vivo.
    Depositor
    Kevin Brindle
    Article
    Rodriguez et al J Pathol. 2014 Mar;232(4):449-57. doi: 1
    Inserts
    CreERT2 with intron
    Firefly luciferase
    mStrawberry
    Use
    Lentiviral and Luciferase; Fluorescence
    Tags
    Expression
    Mammalian
    Mutation
    synthetic intron added as indicated
    Promoter
    CAGGS (after Cre mediated inversion) and CAGGS (f…
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123068-plasmid-map-sequence-id-240523

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  30. pOPINB-hRNF216(511-784)

    Plasmid
    #171923
    Purpose
    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic RBR-helix construct suitable for activity assays.
    Depositor
    Bernhard Lechtenberg
    Article
    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d
    Insert
    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    Use
    Tags
    3C protease cleavage site and 6x-His tag
    Expression
    Bacterial
    Mutation
    codon optimised for expression in E. coli
    Promoter
    T7
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    171923-plasmid-map-sequence-id-346329

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Showing: 2191 - 2220 of 3176 results