We narrowed to 6,273 results for: nls
-
Plasmid#184064Purposecyclofen-inducible Kaede nuclear relocation via mammalian cell transfection or mRNA synthesisDepositorInsertKaede-nls-ERT2
UseSynthetic BiologyExpressionMammalianMutationERT2: G400V, M543A, L544A, deleted 1-281;PromotersCMV+SP6Available SinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-sadCas9-VP64
Plasmid#115790PurposeAn AAV vector expressing S. aureus dCas9 fused to VP64.DepositorInsertSa-dCas9
UseAAVTagsNLS and NLS-VP64ExpressionMammalianPromoterCMVAvailable SinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMP597
Plasmid#134999PurposeInducible ZF-m6A Writer. Use abscisic acid (ABA) to direct GATC methylation to ZF binding sites. pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastRDepositorInsertpMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166-iSpyMac
Plasmid#149553PurposeGateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMsDepositorInsertiSpyMacCas9
UseCRISPR; Gateway compatible zispymaccas9 entry clo…Tags3X FLAG, NLS and NLSExpressionPlantMutationR221K, N394K, Protospacer interacting domain form…Available SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLbCpf1-BE-YEE
Plasmid#154148PurposeExpresses dLbCpf1-BE-YEE(dLbCas12a-BE-YEE) in mammalian cells.DepositorInsertdLbCpf1-BE-YEE (dCas12a-BE-YEE)
TagsSV40 NLSExpressionMammalianMutationAPOBEC1(W90Y+R126E+R132E), dLbCpf1(D832A+E925A+D1…PromoterCMVAvailable SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
SauKKHCas9 PE2*
Plasmid#169852PurposeMammalian Expression, sauCas9-KKH based prime editorDepositorInsertSaCas9KKH-N580A-M-MLV
TagsbpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…ExpressionMammalianPromotercmvAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
HBT_pcoCASphi_version1
Plasmid#197931PurposeCloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminalDepositorInsertpcoCasphi-2
UseCRISPRTagsFLAG tag and SV40 NLS and nucleoplasmin NLSExpressionPlantPromoter35SPPDK promoterAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTK3b_030
Plasmid#123808PurposeEncodes NLS::dCAS9::NLS as a Type 3b part to be used in the MTK systemDepositorInsertNLS::dCAS9::NLS
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Nme2Cas9_AAV
Plasmid#119924PurposeAll-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassetteDepositorInsertNme2Cas9
UseAAVTagsNLS-3xHA-NLS and NLS-NLSExpressionMammalianPromoterU1aAvailable SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-HTNC
Plasmid#13763PurposeFor expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.DepositorInsertHis-TAT-NLS-Cre
UseBaculovirusTagsHis Tag, NLS, and TATExpressionBacterialPromoterp10 promoterAvailable SinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
SauCas9 PE2*
Plasmid#169851PurposeMammalian Expression, sauCas9 based prime editorDepositorInsertSaCas9-N580A-M-MLV
TagsbpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…ExpressionMammalianPromotercmvAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eBE-S3
Plasmid#101093PurposeExpresses eBE-S3 in mammalian cellsDepositorInserteBE-S3
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-SacB-Cas9-T2A-mCherry_(BbsI)
Plasmid#117070PurposeEncodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selectionDepositorInsertsCas9
SacB
UseCRISPRTags3x FLAG, NLS, and T2A-mCherryExpressionMammalianPromoterCBh and U6Available SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR158
Plasmid#240639PurposeExpression of NLS-Kaede in Exaiptasia diaphanaDepositorInsertsAIPGENE865 promoter
Kaede
UseExpression of nls-kaede in exaiptasia diaphanaTagsSV40 NLSMutationnaturally occurring 749-bp insertion at bp 628 (f…PromoterExaiptasia diaphana Elongation Factor 1 alpha (AI…Available SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
BII-tdT-inv-GFP
Plasmid#133393PurposePiggybac vector for bidirectional promoter reporter assayDepositorTypeEmpty backboneExpressionMammalianPromoterNo promoterAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtW-smURFP
Plasmid#133399PurposePiggybac vector for constitutive expression of smURFP-NLSDepositorInsertsmURFP-NLS
ExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT22iuasvApoWtEos3N(#486)
Plasmid#184107Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)MutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …Promoter4xUASAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtW-iRFP670
Plasmid#133397PurposePiggybac vector for constitutive expression of iRFP670-NLSDepositorInsertiRFP-670-NLS
ExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pY125 (pcDNA3.1-huBfCpf1)
Plasmid#92301PurposeExpression of humanized BfCpf1DepositorInserthuBfCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY115 (pcDNA3.1-huMlCpf1)
Plasmid#92275PurposeExpression of humanized MlCpf1DepositorInserthuMlCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY112 (pcDNA3.1-huSaCpf1)
Plasmid#92269PurposeExpression of humanized SaCpf1DepositorInserthuSaCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-eBE-Y130F
Plasmid#113423PurposeExpresses hA3A-eBE-Y130F in mammalian cellsDepositorInserthA3A-eBE-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
UseCRISPRExpressionMammalianMutationhAPOBEC3A_Y130FPromoterCMVAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9 PE2*-3HA
Plasmid#169854PurposeMammalian Expression, SpyCas9 PE2* prime editor with 3HA-tagDepositorInsertSpCas9-H840A-star-M-MLV-3HA
Tags3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…ExpressionMammalianPromotercmvAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-eBE-Y132D
Plasmid#113429PurposeExpresses hA3A-eBE-Y132D in mammalian cellsDepositorInserthA3A-eBE-Y132D (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
UseCRISPRExpressionMammalianMutationhAPOBEC3A_Y132DPromoterCMVAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRD439
Plasmid#168465PurposeFor the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mTurquoise2-H-NSdbd-NLS
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD437
Plasmid#168463PurposeFor the insertion pf NLS-mEos3.2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mEos3.2-H-NSdbd-NLS
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD438
Plasmid#168464PurposeFor the insertion pf NLS-mEos3.2-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mEos3.2-NLS
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD440
Plasmid#168466PurposeFor the insertion pf NLS-mCherry-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mCherry-H-NSdbd-NLS
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD441
Plasmid#168467PurposeFor the insertion pf NLS-mEGFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mEGFP-H-NSdbd-NLS
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD442
Plasmid#168468PurposeFor the insertion of NLS-eYFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-eYFP-H-NSdbd-NLS
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only