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pCXLE-hK + E2-Crimson

Plasmid

#74946

Purpose

Dosage control and tracing of reprogramming episomes

Depositor

Ann Zovein

Article

Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

Insert

Klf4 (KLF4 Human)

Expression

Mammalian

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only
PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

Plasmid

#203228

Purpose

Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Park et al Nat Commun. 2025 Feb 4;16(1):1333. doi:

Insert

Membrane-localized optopatch for neuronal dendrites

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-SynI-CreOn-FlpOff-mNaChBac-P2A-EGFP

Plasmid

#176280

Purpose

Viral vector for co-expression of NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

mNaChBac-P2A-EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

human Synapsin I

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-ZHER-mgSrtA

Plasmid

#125793

Purpose

for co-expression of ZHER (a HER2 binding affibody) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of HER2 positive cells.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

ZHER-mgSrtA

Expression

Mammalian

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-trAtCCD7-OsD27(pYL726 )

Plasmid

#178285

Purpose

Co-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from Arabidopsis

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Inserts

OsD27
trAtCCD7

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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PP023 - CAG::QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX

Plasmid

#182063

Purpose

Co-expression of Arch-based voltage indicator (QuasAr6a) and membrane-localized calcium reporter (jRGECO1a-CAAX)

Depositor

Adam Cohen

Article

Landau et al eLife. 2022 Mar 23;11. pii: 76993. doi:

Insert

QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX

Use

Lentiviral

Promoter

CAG

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+(rInsp)-Ins-GLuc

Plasmid

#89928

Purpose

Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

Depositor

Melanie Cobb

Article

Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

Insert

human insulin-Gaussia-Luciferase (INS Synthetic, Human)

Expression

Mammalian

Mutation

DNA sequence for Gaussia luciferase was humanized…

Promoter

rat Insulin II promoter

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-SynI-CreOn-FlpOff-TeNT-P2A-EGFP

Plasmid

#176282

Purpose

Viral vector for co-expression of TeNT and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

TeNT-P2A-EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

human Synapsin I

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD40L (lose of function mutant)-mgSrtA

Plasmid

#125796

Purpose

for co-expression of a loss of function mutant of CD40L and mgSrtA on cell membrane. A negative control to plasmid pcDNA3.1-CD40L-mgSrtA.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

CD40L (lose of function mutant)-mgSrtA

Expression

Mammalian

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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anti-FIX(a) Emi HC

Plasmid

#113664

Purpose

For transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with the anti-FX(a) Emi HC and the Emi LC.

Depositor

Robert Peters

Article

Leksa et al J Thromb Haemost. 2019 Mar 18. doi: 10.1

Insert

Emicizumab anti-FIX/FIXa heavy chain

Expression

Mammalian

Promoter

CMV Promoter

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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dSpyCas9-p300-P2A-CASANOVA

Plasmid

#113129

Purpose

Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

dCas9-p300-P2A-CASANOVA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC-CAAX

Plasmid

#202719

Purpose

Mammalian co-expression of membrane-bound Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

Depositor

Adam Cohen

Article

Xiang et al bioRxiv 2023

Inserts

Myr-Pink Flamindo
EGFP-bPAC-CAAX

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Solo-mScarlet-LMNB1

Plasmid

#207771

Purpose

Donor template for mScarlet insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

LMNB1 Homology Arms flanking a mScarlet Tag (LMNB1 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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FSW-ALDH2-T2A-EGFP

Plasmid

#197036

Purpose

Express ALDH2 from hSyn1 promoter, co-expressed with EGFP

Depositor

Ronald Hart

Article

Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

Insert

ALDH2 (ALDH2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK-NFLAG-hFUS

Plasmid

#50487

Purpose

Produces lentivirus expressing human FUS with FLAG tag at its N-terminus by co-transfection with psPAX2 and pMD2G

Depositor

Zissimos Mourelatos

Article

Nakaya et al RNA. 2013 Apr;19(4):498-509. doi: 10.126

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

FLAG

Promoter

TRE

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only
pWALIUM-intron 3x UAS attB

Plasmid

#206360

Purpose

includes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequences

Depositor

Erdem Bangi

Article

Teague et al PLoS Genet. 2023 Jun 2;19(6):e1010792. d

Type

Empty backbone

Expression

Insect

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_WTDHFR_WTTS

Plasmid

#91226

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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FGFR1_HUMAN_D0

Plasmid

#79719

Purpose

This plasmid encodes the kinase domain of FGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

FGFR1 (FGFR1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
CDK2_HUMAN_D0

Plasmid

#79726

Purpose

This plasmid encodes the kinase domain of CDK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CDK2 (CDK2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MERTK_HUMAN_D0

Plasmid

#79705

Purpose

This plasmid encodes the kinase domain of MERTK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MERTK (MERTK Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only