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We narrowed to 62,913 results for: cloning

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Showing: 1461 - 1480 of 62913 results

  2. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    86613-plasmid-map-sequence-id-168851

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  11. pcDNA3 tdTomato LIC cloning vector (6F)

    Plasmid
    #86963
    Purpose
    Empty LIC vector; adds a tdTomato gene to the C-terminus of your protein of interest.
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86963-plasmid-map-sequence-id-174746

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  14. enOgeuIscB with ωRNA-v13

    Plasmid
    #220940
    Purpose
    CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v13 scaffold with BsmBI for guide cloing
    Depositor
    Weixin Tang
    Publication
    Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/
    Inserts
    CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
    OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220940-plasmid-map-sequence-id-443125

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  15. c3GIC9

    Plasmid
    #62191
    Purpose
    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes
    Depositor
    Lukas Dow
    Publication
    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do
    Insert
    humanized S. Pyogenes Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Promoter
    TRE3G
    Available since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    62191-plasmid-map-sequence-id-156341

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  16. pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2

    Plasmid
    #236245
    Purpose
    Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter
    Depositor
    Jennifer Lippincott-Schwartz
    Publication
    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do
    Insert
    mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
    Use
    AAV
    Tags
    mTagBFP2
    Promoter
    U6 and human Synapsin1
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    236245-plasmid-map-sequence-id-471239

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Showing: 1461 - 1480 of 62913 results