We narrowed to 87,288 results for: lias

1. ACBE-Q

   Plasmid

   #204608

   Purpose

   Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. VE-cad-eGFP Donor plasmid

   Plasmid

   #92309

   Purpose

   Knock eGFP into human VE-cad locus

   Depositor

   Sean Palecek

   Article

   Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

   Insert

   VEcad Homologous Recombination Arms (CDH5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   about 30 a.a. truncated at end of protein

   Promoter

   Available since

   Aug. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. VE-cad KI gRNA1

   Plasmid

   #92310

   Purpose

   CRISPR-GFP-gRNA for cutting VEcad

   Depositor

   Sean Palecek

   Article

   Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

   Insert

   VEcad KI gRNA1 (CDH5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. VE-cad KI gRNA2

   Plasmid

   #92311

   Purpose

   CRISPR-GFP-gRNA for cutting VEcad

   Depositor

   Sean Palecek

   Article

   Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

   Insert

   VEcad KI gRNA2 (CDH5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-Cre

   Plasmid

   #198712

   Purpose

   For lentiviral expression of Cre recombinase in mammalian cells.

   Depositor

   Tyler Jacks

   Article

   Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

   Insert

   Pgk-Cre
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-CMVc-Cas9

   Plasmid

   #106431

   Purpose

   Expresses SpCas9 from the CMVc promoter in AAV backbone

   Depositor

   Juan Belmonte

   Article

   Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

   Insert

   Cas9
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMVc

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-dLight1.3b

   Plasmid

   #125560

   Purpose

   For expression of the genetically encoded dopamine sensor dLight1.3b

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   dLight1.3b
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEMS2115

   Plasmid

   #49140

   Purpose

   The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   ssAAV-Ple155-emGFP-WPRE
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MiniPromoter Ple155 from the human PCP2 gene

   Available since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pNTI194

   Plasmid

   #84266

   Purpose

   Expresses Avi-tagged RPL1 and humanized birA

   Depositor

   Nicholas Ingolia

   Article

   Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:

   Inserts

   RPL1 (RPL10A Human, (*see below))
   humanized birA
   

   Use

   Tags

   Avi tag, Flag, Gly/Ser linker, and TEV site

   Expression

   Mammalian

   Mutation

   R33A, A34E (*see below)

   Promoter

   CMV and P2A peptide

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. CRY-Gal∆DD (B1013)

    Plasmid

    #92035

    Purpose

    Expresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRES

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fusion of plant CRY2 to Gal4 binding domain (AA1-…

    Promoter

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. CIB-VP64 (B1016)

    Plasmid

    #92037

    Purpose

    Expresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CIB1-VP64 (CIB1 Mustard Weed)
    

    Use

    Tags

    Fusion of plant CIB1 with VP64 activation domain.…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV302

    Plasmid

    #119943

    Purpose

    Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

    Insert

    N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV312.3

    Plasmid

    #119944

    Purpose

    Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

    Insert

    C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-nEF-dCas9

    Plasmid

    #106430

    Purpose

    Expresses SpdCas9 from the nEF promoter in AAV backbone

    Depositor

    Juan Belmonte

    Article

    Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

    Insert

    nEF-dCas9
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    nEF

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-CLK1

    Plasmid

    #174088

    Purpose

    Inducibly expresses Myc-CLK1 in mammalian cells with the Tet-on system

    Depositor

    Wesley Sundquist, Katie Ullman

    Article

    Williams et al Elife. 2021 Aug 4;10. pii: 63743. doi: 1

    Insert

    CLK1 (CLK1 Human)
    

    Use

    Lentiviral

    Tags

    Myc-GSSS

    Expression

    Mammalian

    Mutation

    Promoter

    TREtight

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCas9-T2A-mApple

    Plasmid

    #193780

    Purpose

    Tricistronic expression of Cas9-mApple-BSD in mammalian cells

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    mApple
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with Cas9

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Alpha MHC-DN.JNK2

    Plasmid

    #51931

    Purpose

    mammalian expression of dominant negative JNK2

    Depositor

    Jeffery Molkentin

    Article

    Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

    Insert

    DN JNK2 (Mapk9 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    dominant negative: the dual phosphorylation moti…

    Promoter

    cardiac-specific α-myosin heavy chain 5.5 kb pro…

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pTBL680 CHYRON3 integration construct

    Plasmid

    #126448

    Purpose

    To integrate the CHYRON3 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6/3xLacO-CHYRON3 hgRNA
    pCMV-puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6/3xLacO

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEMS1985

    Plasmid

    #49116

    Purpose

    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    ssAAV-Ple155-iCre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MiniPromoter Ple155 from the human PCP2 gene

    Available since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pLL5.0 hCortKD-Flcortmut3YD EGFP

    Plasmid

    #187264

    Purpose

    Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    Cortactin (CTTN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    3YD (Y412, Y470, Y486)

    Promoter

    U6, 5'LTR

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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