We narrowed to 162,094 results for: addgene

1. HO-pGAL-poly-KanMX4-HO

   Plasmid

   #51664

   Purpose

   yeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoter

   Depositor

   David Stillman

   Article

   Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

   Type

   Empty backbone

   Use

   Yeast integration

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. 5KO9 (HMCES)

   Plasmid

   #101877

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HMCES (HMCES Human)
   

   Use

   Tags

   6x His (modified C-term tag is AENLYFQSHHHHHH)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pNM3725

   Plasmid

   #154113

   Purpose

   UAS 11X ∆pes-10 promoter driving GFP-C1 in an RCME vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   UAS 11X::∆pes-10::GFP-C1::tbb-2 3'
   

   Use

   Rmce

   Tags

   Expression

   Worm

   Mutation

   Promoter

   not applicable

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDD379

   Plasmid

   #91834

   Purpose

   SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

   Plasmid

   #66708

   Purpose

   expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

   Depositor

   Charles Gersbach

   Insert

   S.Pyogenes VP64-dCas9-VP64
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   VP64

   Expression

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. AMPKA2

   Plasmid

   #36194

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   AMPKA2 (PRKAA2 Human)
   

   Use

   Tags

   His tag with thrombin cleavage

   Expression

   Bacterial

   Mutation

   contains KINASE DOMAIN only

   Promoter

   Available since

   Aug. 8, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

7. 7TC

   Plasmid

   #24315

   Depositor

   Roel Nusse

   Article

   ten Berge et al Cell Stem Cell. 2008 Nov 6. 3(5):508-18.

   Insert

   7xTcf-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. 6CCR (GID4)

   Plasmid

   #119960

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   GID4 (C17orf39 Human)
   

   Use

   Tags

   6xHIS, thrombin,SUMO and TEV cleavage

   Expression

   Bacterial

   Mutation

   contains amino acids 116-300

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pSADdeltaG-BFP

   Plasmid

   #32639

   Depositor

   Edward Callaway

   Article

   Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

   Insert

   BFP
   

   Use

   Rabies virus

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICH-roGFP2-synORP1

    Plasmid

    #191680

    Purpose

    Ti plasmid pICH86988 harbouring roGFP2-synORP1 for H2O2 detection in plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    rredox(roGFP2-synORP1
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. JAK3 (JAK3A-c023)

    Plasmid

    #98229

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JAK3A (JAK3 Human)
    

    Use

    Baculovirus

    Tags

    His-6-TEV

    Expression

    Mutation

    pfam00069:Pkinase 822-1091

    Promoter

    polyhedrin

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p6VP1

    Plasmid

    #24725

    Depositor

    Christopher Buck

    Article

    Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

    Insert

    VP1 from Human Polyomavirus 6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFtnA-Halo

    Plasmid

    #98281

    Purpose

    mammalian expression of FtnA-Halo

    Depositor

    Mike Heilemann

    Article

    Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

    Insert

    FtnA
    

    Use

    Tags

    Halo-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. p7VP1

    Plasmid

    #24726

    Depositor

    Christopher Buck

    Article

    Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

    Insert

    VP1 from Human Polyomavirus 7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid

    #218710

    Purpose

    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. VV063: 1xCox8 - superecliptic pHluorin in fck

    Plasmid

    #58500

    Purpose

    Expresses the pH sensor superecliptic pHluorin in the mitochondria of mammalian cells

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    1xCox8 - Superecliptic pHluorin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNM3814

    Plasmid

    #154122

    Purpose

    mec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    mec-4p::rtetR-L-QFAD::act-4 '3
    

    Use

    Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTP-AAV-ChATen-mCherry

    Plasmid

    #193741

    Purpose

    This AAV vector contains a ChAT enhancer that drives mCherry expression in spinal cord skeletal motor neurons. Specificity of vector not characterized outside the mouse spinal cord.

    Depositor

    Hynek Wichterle

    Insert

    ChAT enhancer, mini promoter, synthetic intron, mCherry
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    1000 bp ChAT enhancer with a 32 bp minimal promot…

    Available since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGlnA-Ypet

    Plasmid

    #98278

    Purpose

    mammalian expression of GlnA-Ypet

    Depositor

    Mike Heilemann

    Article

    Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

    Insert

    GlnA
    

    Use

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDD121

    Plasmid

    #91833

    Purpose

    Cas9 expression in C. elegans

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Codon optimized and with synthetic introns for C.…

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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