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Plasmid#211790PurposepDonorTp with addition of fugfp gene from pUS252 outside of the FRT sitesDepositorInsertfuGFP
UseTagsExpressionMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT_H1-S1
Plasmid#218400Purpose3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in first intronDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DDX53-FLAG
Plasmid#222241PurposeExpress FLAG tagged DDX53 in mammalian cellsDepositorInsertDDX53 (DDX53 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterT7Available sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29a-DDX53-Q249A
Plasmid#222216PurposeExpress DDX53 mutant Q249A protein in bacteriaDepositorInsertDDX53_Q249A (DDX53 Human)
UseTagsHis TagExpressionBacterialMutationK272APromoterT7Available sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29a-DDX53-K272A
Plasmid#222215PurposeExpress DDX53 mutant K272A protein in bacteriaDepositorInsertDDX53_K272A (DDX53 Human)
UseTagsHis TagExpressionBacterialMutationK272APromoterT7Available sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29a-DDX53-E377Q
Plasmid#222217PurposeExpress DDX53 mutant E377Q protein in bacteriaDepositorInsertDDX53_E377Q (DDX53 Human)
UseTagsHis TagExpressionBacterialMutationE377QPromoterT7Available sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DDX53-GFP
Plasmid#222239PurposeExpress GFP tagged DDX53 in mammalian cellsDepositorInsertDDX53 (DDX53 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMV and T7Available sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DDX53-K272A
Plasmid#222242PurposeExpress FLAG tagged DDX53 mutant K272A in mammalian cellsDepositorInsertDDX53 (DDX53 Human)
UseTags3xFLAGExpressionMammalianMutationK272APromoterT7Available sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT_S1-H1
Plasmid#218392Purpose3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in second intronDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
mPLD3-sgRNA-Cas9-mcherry
Plasmid#199700Purposeencodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherryDepositorInserthSpCas9
UseCRISPRTagsP2A-RFPExpressionMammalianMutationPromoterU6 promoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-N
Plasmid#218398Purpose3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_N-H1
Plasmid#218397Purpose3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT_S7-H1
Plasmid#218393Purpose3-exon minigene, with SRSF7 motif-rich middle exon, and hnRNPA1 motif-rich region in second intronDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR6K-GFP-CASTIF
Plasmid#199653PurposeR6K plasmid encoding a type I-F CAST but no CRISPR arrayDepositorInsertCAST I-F systems
UseTagsExpressionBacterialMutationPromoterJ23119 promoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR6K-GFP-CASTV
Plasmid#199657PurposeR6K plasmid encoding a type V CAST but no CRISPR arrayDepositorInsertCAST V systems
UseTagsExpressionBacterialMutationPromoterJ23119 promoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCLX-KRT7-GFP
Plasmid#216853PurposeThis plasmid is for the production of lentiviral vectors that can be used to identify and live sort pancreatic progenitor expresssing the Keratin7 gene.DepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterKeratin7Available sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-C
Plasmid#213496PurposeEncodes Node C of the three-node Turing circuitDepositorInsertslambda cI 5G6GP
mCherry
UseSynthetic BiologyTagsASV tag and LVA tagExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR Ef1a-dCas9-BFP-KRAB
Plasmid#217304Purposelentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoterDepositorInsertdCas9-tagBFP-KRAB
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMutationPromoterEf1alphaAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA Display Part 1-4 DO
Plasmid#206514PurposeCcdB Dropout vector for parts 1-4 of Hemagglutinin surface expression in mammalian cellsDepositorTypeEmpty backboneUseTags3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…ExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ORF3cStrep
Plasmid#208728PurposeExpresses ORF3c-Strep in mammalian cellsDepositorInsertORF3c (ORF3a )
UseTagsStrepExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
p_L_3c_SG_RLuc
Plasmid#208729PurposeFor assessing leaky scanning rates in mammalian cellsDepositorInsertORF3c (ORF3a )
UseTagsRenilla luciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHflu3
Plasmid#203881PurposepHI1 with insertion of kanamycin-resistance geneDepositorInsertaminoglycoside O-phosphotransferase APH(3')-IIa
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-P_TALEsp1_PUPsp1-lacI_Ptac-lacO
Plasmid#204079PurposeType 0 promoter partDepositorInsertTALEsp1-stabilized promoter driving lacI repressor; Ptac-lacO IPTG-inducible promoter
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-iRFP713(C15S/V256C)-P2A-mCherry
Plasmid#198069PurposeMammalian expression of iRFP713(C15S/V256C) and EGFPDepositorInsertiRFP713(C15S/V256C)
UseTagsP2A-mCherryExpressionMammalianMutationC15S/V256C mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-iRFP713(C15S/V256C)-P2A-EGFP
Plasmid#198062PurposeThe lentiviral vector for iRFP713(C15S/V256C) and EGFPDepositorInsertiRFP713(C15S/V256C)
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationC15S/V256C mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-miRFP670nano(R57C/C86S)-P2A-EGFP
Plasmid#197370PurposeThe lentiviral vector for miRFP670nano(R57C/C86S) and EGFPDepositorInsertmiRFP670nano(R57C/C86S)
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationR57C/C86S mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-6504-P2A-EGFP
Plasmid#197368PurposeThe lentiviral vector for NIR-GECOHI and EGFPDepositorInsertNIR-GECO2(C18S/I426C)
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationC18S/I426C mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-HCV_NS3-GS-NS4A
Plasmid#203504PurposeExpresses HCV NS3-NS4A protease (with GS linker) from a GAL promoter with a URA3 markerDepositorInsertHCV NS3-GS-NS4A
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-HPeV_3C
Plasmid#203465PurposeExpresses HPeV 3C protease from a GAL10 promoter with a URA3 markerDepositorInsertHPeV 3C protease (polyprotein gene )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G2-Km ymTurquoise2
Plasmid#193958PurposeTEF1 promoter controlled expression plasmid with G418 resistance for expressing ymTurquoise2 in budding yeastDepositorInsertymTurquoise2
UseTagsExpressionYeastMutationPromoterTEF1Available sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn1-ChR2(H134R)-EYFP
Plasmid#197593PurposeLentiviral transfer vector for the neuronal expression of ChR2(H134R)-EYFP (Channelrhodopsin for the inducing neuronal activity)DepositorInsertChR2(H134R)-EYFP
UseLentiviralTagsExpressionMutationPromoterhSynIAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAS-mak10 gRNA
Plasmid#160385PurposeExpresses guide RNA for targeting MAK10 in yeastDepositorInsertmak10 gRNA
UseTagsExpressionYeastMutationPromoterRNA pol III promoter (tRNA-Tyr)Available sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB4A3-PBAD-phzSM
Plasmid#165620PurposeArabinose-inducible expression of phzSM for conversion of phenazine-1-carboxylic acid to pyocyaninDepositorUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1 Danio rerio CBS-pair domain of CNNM4
Plasmid#170785PurposeExpresses pGEX-6P1 Danio rerio CBS-pair domain of CNNM4DepositorInsertCBS from CNNM4
UseTagsGSTExpressionBacterialMutationNAPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
R6K_BAD_GFP-mCherry_ChInt++
Plasmid#187393Purposechromosomal integration downstream of glmS gene via Tn7 system of a cassette encoding for arabinose-inducible GFP and mCherryDepositorInsertGFP, mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
R6K_BAD_GFP-mCherry_ChInt--
Plasmid#187394Purposechromosomal integration downstream of glmS gene via Tn7 system of a cassette encoding for arabinose-inducible GFP and mCherryDepositorInsertGFP, mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
R6K_BAD_mCherry-GFP_ChInt--
Plasmid#187396Purposechromosomal integration downstream of glmS gene via Tn7 system of a cassette encoding for arabinose-inducible GFP and mCherryDepositorInsertGFP, mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB258
Plasmid#185072PurposeBacterial expression of N-terminus and FXFG repeats of NUP1 nucleoporin as GST-Nup1-N+Rep fusionDepositorInsertNUP1
UseTagsGSTExpressionYeastMutationNup1 N-term+FXFG repeatsPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-mVenus-TrkC
Plasmid#182948PurposeTo express the rat TrkC fused in the N-terminus with mVenusDepositorInsertmVenus-TrkC (Ntrk3 Rat, Synthetic)
UseRetroviral and Synthetic BiologyTagsmVenusExpressionMammalianMutationPromoterMMLVAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGAE-UFO.664
Plasmid#183471PurposeLentiviral vector expressing HIV Envelope UFO.664DepositorInsertHIV envelope
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only