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Showing: 3701 - 3750 of 4075 results

  2. KBB556

    Plasmid
    #185108
    Purpose
    1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with S158A, F159A, F160A
    Depositor
    Ken Belanger
    Article
    Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722
    Insert
    POM152
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    1st 200 amino acids (N-term + transmembrane domai…
    Promoter
    Available since
    Aug. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    185108-plasmid-map-sequence-id-368110

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  3. KBB553

    Plasmid
    #185107
    Purpose
    1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with Y180L, Q182L
    Depositor
    Ken Belanger
    Article
    Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722
    Insert
    POM152
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    1st 200 amino acids (N-term + transmembrane domai…
    Promoter
    Available since
    Aug. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    185107-plasmid-map-sequence-id-368005

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  10. KBB394

    Plasmid
    #185086
    Purpose
    NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)
    Depositor
    Ken Belanger
    Article
    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4
    Insert
    NMA111
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Nma111 KKR 9-11 AAA; KRK 28-30 AAA
    Promoter
    Available since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    185086-plasmid-map-sequence-id-367451

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  17. VanR - F165V

    Plasmid
    #158965
    Purpose
    Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.
    Depositor
    Michael Jensen
    Article
    et al Biotechnology Notes, Volume 1, 2020, Pag
    Insert
    VanR - F165V
    Use
    Tags
    Expression
    Yeast
    Mutation
    Wild type VanR from Caulobacter crescentus with m…
    Promoter
    TEF1
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    158965-plasmid-map-sequence-id-319279

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  18. VanR - F165R

    Plasmid
    #158966
    Purpose
    Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.
    Depositor
    Michael Jensen
    Article
    et al Biotechnology Notes, Volume 1, 2020, Pag
    Insert
    VanR - F165R
    Use
    Tags
    Expression
    Yeast
    Mutation
    Wild type VanR from Caulobacter crescentus with m…
    Promoter
    TEF1
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    158966-plasmid-map-sequence-id-319292

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  22. pMET17p-CCG 316

    Plasmid
    #131166
    Purpose
    For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant
    Depositor
    Nils Johnsson
    Article
    Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:
    Type
    Empty backbone
    Use
    Tags
    CCG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    131166-plasmid-map-sequence-id-255869

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  23. Spt2-K166R-GFP

    Plasmid
    #115573
    Purpose
    Expresses yeast Spt2-GFP fusion protein mutated from lysine to arginine at site 166
    Depositor
    Michael Downey
    Article
    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62
    Insert
    SPT2
    Use
    Tags
    GFP
    Expression
    Bacterial and Yeast
    Mutation
    Lysine 166 mutated to Arginine
    Promoter
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  24. GFP-X2

    Plasmid
    #115567
    Purpose
    GFP tethered to two repeats of the Gcn5 consensus motif
    Depositor
    Michael Downey
    Article
    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62
    Insert
    Green Fluorescent Protein
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Two repeats of the Gcn5 consensus motif are tagge…
    Promoter
    ADH1
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    115567-plasmid-map-sequence-id-226345

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  25. GFP-X1

    Plasmid
    #115566
    Purpose
    GFP tethered to a single repeat of the Gcn5 consensus motif
    Depositor
    Michael Downey
    Article
    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62
    Insert
    Green Fluorescent Protein
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Single Gcn5 consensus motif repeat tethered to C-…
    Promoter
    ADH1
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    115566-plasmid-map-sequence-id-226330

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  26. Hsh155 N4A 3xFLAG

    Plasmid
    #111975
    Purpose
    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker. It has 3xFLAG at the C-terminus. Contains the N4A mutation.
    Depositor
    Aaron Hoskins
    Article
    Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d
    Insert
    Hsh155 N4A 3x FLAG
    Use
    Tags
    3xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    T178A R181A R182A R186A
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111975-plasmid-map-sequence-id-213742

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  27. Hsh155 K818A 3xFLAG

    Plasmid
    #111982
    Purpose
    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains K818A mutation. Lethal
    Depositor
    Aaron Hoskins
    Article
    Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d
    Insert
    Hsh155 K818A 3x FLAG
    Use
    Tags
    3xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    K818A
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111982-plasmid-map-sequence-id-213737

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  28. Hsh155 R775A 3xFLAG

    Plasmid
    #111977
    Purpose
    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R775A mutation. Lethal
    Depositor
    Aaron Hoskins
    Article
    Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d
    Insert
    Hsh155 R775A 3x FLAG
    Use
    Tags
    3xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    R775A
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111977-plasmid-map-sequence-id-213729

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  29. Hsh155 R778A 3xFLAG

    Plasmid
    #111979
    Purpose
    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R778A mutation. Lethal
    Depositor
    Aaron Hoskins
    Article
    Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d
    Insert
    Hsh155 R778A 3x FLAG
    Use
    Tags
    3xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    R778A
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111979-plasmid-map-sequence-id-213728

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  33. pDA186

    Plasmid
    #74250
    Purpose
    Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pGPD1 (UbiY 2xNLSs SZ3).
    Depositor
    Serge Pelet
    Article
    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10
    Insert
    Inducible peptide of the dPSTR
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    pGPD1
    Available since
    May 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74250-plasmid-map-sequence-id-139759

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  34. pDA171

    Plasmid
    #74251
    Purpose
    Intermediate cloning vector, with MCS2 containing inducible stable peptide driven by pSTL1 (Venus 2xNLSs SZ1).
    Depositor
    Serge Pelet
    Article
    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10
    Insert
    Inducible peptide of the dPSTR
    Use
    Tags
    Venus
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    pSTL1
    Available since
    May 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74251-plasmid-map-sequence-id-139758

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  48. pCRCT

    Plasmid
    #60621
    Purpose
    Plasmid encoding iCas9, tracrRNA and crRNAs
    Depositor
    Huimin Zhao
    Article
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60621-plasmid-map-sequence-id-208224

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Showing: 3701 - 3750 of 4075 results