We narrowed to 2,606 results for: EXO

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pX330_ACTB-i2 sgRNA / hSpCas9

Plasmid

#172826

Purpose

Mammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-EGxxFP-PIGA

Plasmid

#153958

Purpose

Used for EGxxFP assay with PIGA intron 5 and exon 6 sequences

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

PIGA (a 401-bp fragment from intron 5 and exon 6)

Use

A plasmid specific for egxxfp assay

Tags

N/A

Expression

Mammalian

Mutation

No mutation

Promoter

CAG promoter

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-EGxxFP-CD55

Plasmid

#153959

Purpose

Used for EGxxFP assay with CD55 intron 3, exon 4, and intron 4 sequences

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

CD55 (a 1,016-bp fragment from intron 3, exon 4, and intron 4)

Use

A plasmid specific for egxxfp assay

Tags

N/A

Expression

Mammalian

Mutation

No mutation

Promoter

CAG promoter

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-Neo-p53mut

Plasmid

#99239

Purpose

Mutant p53 encoded by AS-30D rat hepatoma cell line

Depositor

Peter Pedersen

Article

Mathupala et al J Biol Chem. 1997 Sep 5;272(36):22776-80

Insert

p53 (Tp53 Rat)

Expression

Mammalian

Mutation

Silent mutations in translated protein at Gly (10…

Promoter

CMV immediate/early enhancer/promoter

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SPA mimic WT

Plasmid

#92350

Purpose

To mimic SPA RNA processing and expression

Depositor

Ling-Ling Chen

Article

Wu et al Mol Cell. 2016 Nov 3;64(3):534-548. doi:

Insert

SNRPN exon 9, intron9, exon10, intron10 partial partial of SPA1 (SNRPN Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 MCS-Laccase2 608-1097

Plasmid

#69890

Purpose

Expresses the Drosophila laccase2 exon 2 circular RNA

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

Laccase2 (stw Fly)

Expression

Insect

Mutation

L158I in laccase2

Promoter

Metallothionein Promoter (pMT)

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pMA3411

Plasmid

#46877

Purpose

A retroviral vector for the constitutive expression of soluble guanylate cyclase1 beta3 double mutant (E473K and C541D)

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase1 beta 3 (Gucy1b3 Rat)

Use

Retroviral

Mutation

E473K, C541D

Promoter

LTR

Available Since

Aug. 14, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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p414GPD-3xFLAG-Nop4 dE5

Plasmid

#169263

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 5

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Nop4 delta E5 (NOP4 Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

deleted region corresponding to human Exon 5

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-Nop4 dE8

Plasmid

#169264

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 8

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Nop4 delta E8 (NOP4 Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

deleted region corresponding to human Exon 8

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-RBM28 dE5

Plasmid

#169267

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 5

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 delta E5 (RBM28 Human)

Tags

3xFLAG

Expression

Yeast

Mutation

deleted Exon 5

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-RBM28 dE8

Plasmid

#169268

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 8

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 delta E8 (RBM28 Human)

Tags

3xFLAG

Expression

Yeast

Mutation

deleted Exon 8

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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pCDNA_MET_D14_3Flag

Plasmid

#182495

Purpose

Contains cDNA of the MET gene without the exon 14

Depositor

Paolo Comoglio

Article

Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

Insert

MET (MET Human)

Tags

C-terminal 3xFlag tag

Expression

Mammalian

Mutation

Exon 14 deleted

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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polyQ74-GFP

Plasmid

#231893

Purpose

Forms cytosolic HTT partial exon 1 Q74 aggregates

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q74 (HTT Human)

Tags

EGFP

Expression

Mammalian

Mutation

CAG repeat expansion

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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EPS15-GFP-His

Plasmid

#170860

Purpose

Mammalian expression of EPS15-GFP-His.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Epidermal growth factor receptor pathway substrate 15 (EPS15) (EPS15 Human)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA1-Tat

Plasmid

#138478

Purpose

Expresses HIV Tat for efficient sgRNA packaging.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

Tat

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAP05

Plasmid

#186261

Purpose

sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

Depositor

Yongku Cho

Article

Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

Inserts

sfGFP
EL222 (ELI_04755 Erythrobacter litoralis)

Expression

Bacterial

Promoter

BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shcGAS-Hsa

Plasmid

#128175

Purpose

Doxycyclin inducible shRNA knockdown of human cGAS gene

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shcGAS Mmu (CGAS Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-polyQ23-NLS

Plasmid

#231891

Purpose

Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q23-NLS (HTT Human)

Tags

EGFP and SV40 NLS

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SuExp-hPLD3

Plasmid

#201243

Purpose

express full length human PLD3 protein

Depositor

David Nemazee

Article

Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

Insert

PLD3 (PLD3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pASK-mSAN1-Streptag

Plasmid

#117165

Purpose

Expresses Strep-tagged murine SAN1 nuclease in bacteria

Depositor

Ian Macara

Article

Andrews et al Nat Commun. 2018 Jul 3;9(1):2592. doi: 1

Insert

mSAN1 (Fam120b Mouse)

Tags

Streptag

Expression

Bacterial

Promoter

tetR/tetA

Available Since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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